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Showing 1 to 20 of 23 for “"profilin"”.
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Profilin II : novel functions in membrane trafficking
Profilin II - Novel functions in membrane-trafficking Ralph Gareus - EMBL Mouse Biology Programme, Monterotondo, Italy Profilin is an actin-binding protein expressed in all eukaryotic cells, regulating microfilament remodelling. Besides actin, it binds to poly-L-proline and phosphatidylinositol …
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Transcriptional Regulation of Profilin Is Required For Drosophila Larval Wound Closure
… that chickadee, which encodes the Drosophila Profilin, a protein important for actin filament recycling and cell migration during development, is required for the physiological process of larval epidermal wound closure. After injury, chickadee is transcriptionally upregulated in cells proximal …
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Charakterisierung des Zytoskelett-Proteins Villidin und einer dritten Profilin-Isoform in Dictyostelium discoideum
… novel cytoskeleton protein villidin and a third profilin isoform. One goal of the project was to analyze the features of the villidin sequence in detail and to investigate the protein by molecular, biochemical and cell biological approaches. Villidin has a calculated molecular mass of 190,000 Da. …
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An Optical Flow Based Methodology for Visualizing Dynamic Sucellular [sic] Organiztion [sic] Demonstrated Through Profilin and Rho GTPase Microdomains
… This tool enabled us to discover that Profilin fluctuations are organized in living cells. This organization was found to be dependent on cell polarization and actin binding capability. Expanding on this ability to query all subcellular locations, we developed a feature set and feature …
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Functional studies of selected actin binding proteins by point mutations and GFP fusions
Profilin is an ubiquitous cytoskeletal protein whose function is fundamental to the maintenance of normal cellular physiology. Site-directed mutagenesis of profilin II from Dictyostelium discoideum by PCR resulted in the point mutations W3N and K114E, whereby the W3N profilin is no longer able to …
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Genetic studies on functional redundancy between profilin1 and profilin2 im mice and the role of the profilin ligand Mena in neuronal cell function and mouse behavior
Genetic studies on functional redundancy between profilin1 and profilin2 in mice and the role of the profilin ligand Mena in neuronal cell function and mouse behavior Profilin1 and profilin2 are two actin-binding proteins. Biochemically, proflin1 and profilin2 are similar in respect to their …
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Mechanisms and Functional Consequences of Cardiac Remodeling: Role of Myocardial Rac1 and Vascular Profilin1 Genes
… (LV) hypertrophy. Previously, we have shown that profilin-1 overexpression in the blood vessel medial layer of transgenic mice favoring F-actin induces stress fiber formation and plays an important role in vascular hypertrophy by inducing internal mechanical stress, leading to vascular remodeling …
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Simulated impact trauma and osteoarthritis: the role of cell volume and mechanotransduction
… responsible for actin treadmilling cofilin, profilin and gelsolin mRNA was compared to untreated chondrocytes. REV5901 was observed to reduce cofilin (known actin depolymerizing factor) with 30 % (p<0.05) and increase profilin significantly by 75 % (p<0.001) respectively. Western blot …
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Biochemical and Microscopic Characterization of INFT-1: an Inverted Formin in C. elegans
… elongation rates in the absence or presence of profilin. Evidences demonstrated that INFT-1 is non-processive, indicating a unique mechanism of nucleation. INFT-1 nucleation efficiency is similar to the efficiency of Arabidopsis FORMIN1 (AFH1), another non-processive formin. High phosphate …
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Actin polymerization dynamics at the leading edge
… microscopy of actin filaments, we tested how profilin, Arp2/3, and capping protein (CP) function together to propel beads or thin glass nanofibers coated with N-WASP WCA domains. During sustained motility, physiological concentrations of Mg²⁺ generated actin filament bundles that processively …
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Food Allergens and allergenicity of glycine max (L.) merr. (soy)
… and I?-chains, Gly m Bd30K, trypsin inhibitor, profilin, Gly m 4) and three putative (cysteine protease, isoflavone reductase, malate dehydrogenase) soy allergens were produced as recombinants, and used in an immunoassay to screen 84 soy-specific sera. The three putative allergens were verified …
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Charakterisierung der endosomalen Membranproteine DdLmp B/C aus Dictyostelium discoideum und biochemische Analyse der Stimulierung der bakteriellen Kinase YopO aus Yersinia enterocolitica durch Aktin
… Gruppe zur Suppression des Phänotyps einer Profilin-minus Mutante. Im Zuge der vollständigen Sequenzierung des D. discoideum-Genoms konnte festgestellt werden, daß es neben diesem DdLmpA noch die beiden weiteren homologen Proteine DdLmpB und DdLmpC gibt. Da der Mechanismus der Suppression …
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Nuclear accumulation of G-actin in cellular senescence: Reduced expressions of exportin 6 and Ran small G protein
… eukaryotes. Exp6 mediates nuclear export of profilin-actin complexes. We earlier reported that actin was accumulated in nucleus during replicative senescence of human diploid fibroblast (HDF). In the present study, we found that the increase of doubling time of HDF cells decreased exp6 and …
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Java-based modeling of the actin polymerization cycle
… describes the effects of regulatory proteins (profilin, beta-thymosin, cofilin, and capping protein) on the steady-state actin cycle. The model necessitates the simultaneous solving of 90-330 differential equations and 51-171 additional equations. One object of this thesis was to prove that …
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Wirtsspezifität der Gattung Legionella und Etablierung von Dictyostelium discoideum als Wirtsmodell
… hackeliae. Die Untersuchung einer spezifischen Profilin-minus Dictyostelium-Mutante offenbarte eine erhöhte Phagozytoserate von Legionellen durch die Wirtszellen. Daraus resultierte auch eine leicht gesteigerte intrazelluläre Vermehrungsrate dieser Bakterien. Profilin ist ein Aktin-bindendes …
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Funktionelle Charakterisierung von Myosin IK in Dictyostelium discoideum
… wurde in vitro direkt als Bindung über Profilin bestätigt. Die Ansequenzierung weiterer Liganden führte zu der Identifizierung von D. discoideum Abp1, einem actinbindenden Protein, das bisher nur in S. cerevisiae und M. musculus beschrieben ist. Damit eröffnete sich eine neue Sicht …
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A Molecular Basis for Divergent, Unstable Actin Filaments in Toxoplasma gondii
… However, <italic>T. gondii</italic> profilin: TgPRF) acts to sequester TgACTI from the growing filament. Finally, examination of concentration dependent polymerization has revealed evidence that TgACTI is utilizing an isodesmic mechanism for polymerization. Collectively, these results …
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MOLECULAR MECHANISMS OF ACTIN FILAMENT ASSEMBLY AND TURNOVER
… barbed end, as well as formin with incoming profilin actin reveal a step-by-step mechanism of actin polymerization. In addition, we show how inverted formin-2 (INF2) is uniquely adapted for filament severing and why other formins are not. At the pointed end, it was typically thought that only …
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Interaktion des Proteins ActA von Listeria monocytogenes mit dem Wirtszellprotein LaXp180
… ActA und beschleunigt durch die Rekrutierung von Profilin den Prozeß der Aktinpolymerisation. Der Arp2/3-Komplex interagiert mit dem N-terminalen Bereich von ActA und initiiert die eigentliche Aktin-Polymerisation. Um weitere eukaryotische, mit ActA interagierende Proteine (AIPs) zu isolieren, …
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Identification of cellular signaling events dysregulated in Huntington’s disease.
… points and LIMK1/cofilin/slingshot along with profilin showed alterations in the later time points especially the 3w and 4w, when the mutant huntingtin protein (mHtt) appears in the striatum the most affected cell type in HD brain. Collectively, these data highlight the potential role of …
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