Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"processivity"”.
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Comparative Studies of Processivity Clamp Loader ATP Site Function
… ring-shaped sliding clamps to ensure polymerase processivity. An ATP-dependent clamp loader topologically links the clamp around DNA in a multi-step mechanism. Clamp loaders are ring-shaped pentamers of AAA+ subunits. The pentamer complex contains 3-4 ATP sites, each located at the interface of …
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KSHV encoded viral processivity factor, ORF59, regulates multiple pathways for viral replication
… risk for developing lethal cancers. ORF59, a DNA processivity factor of the viral DNA polymerase, is essential for lytic reactivation and is highly expressed during de novo primary infection. To better understand the role of ORF59, we performed an ORF59-immunoprecipitation from KSHV infected cells …
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The effect of single-stranded DNA binding protein RPA2 on XPD helicase processivity
… two inherent states of unwinding, high and low processivity, and that RPA2 aids unwinding by increasing the likelihood of XPD being in its more processive state.
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Lon degrades stable substrates slowly but with enhanced processivity, redefining the attributes of a successful AAA+ protease
… and substrate contacts; and (iii) exhibits high processivity and frequently completely degrades multi domain substrates. Importantly, despite being a slower unfoldase and translocase than other well characterized AAA+ proteases, Lon is more persistent and successful at degrading targeted …
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RNA replication by poliovirus RNA-dependent RNA polymerase: effects of residue 5 on elongation complex stability and processivity
… relationship related to initiation and processivity of the PV RdRP. Specifically, my work shows that the PV RdRP residue 5 is involved in forming and maintaining the stable, elongation-competent complex. Also, the complex stability can be modulated by downstream RNA interactions or by …
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FIV-FCA and FIV-PCO Reverse Transcriptases: Expression, Purification and Activity Comparison
… history with its host will have higher processivity. In this thesis we expressed, purified and compared the processivity of recombinant His-tagged RTs of FIV-Fca and FIV-Pco. Both of the RTs contained a hexa N-terminal His tag and were expressed in E.coli. Both RT subunits were …
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FIV-FCA and FIV-PCO Reverse Transcriptases: Expression, Purification and Activity Comparison
… history with its host will have higher processivity. In this thesis we expressed, purified and compared the processivity of recombinant His-tagged RTs of FIV-Fca and FIV-Pco. Both of the RTs contained a hexa N-terminal His tag and were expressed in E.coli. Both RT subunits were …
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Structure-Substrate Binding Relationships of HIV-1 Reverse Transcriptase
… affects the enzyme activity. The increase in processivity by p51 in the p66/p51 heterodimer was also demonstrated. These observations suggested that the integrity of p51 is important in subunit-interactions for maintaining a favorable conformation of the enzyme for optimal function. C-terminal …
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Mechanism of Transcription Arrest By The Nun Protein of Bacteriophage HK022
… to regulatory factors that alter the rate and processivity of gene expression (Roberts et al, 2008). Escherichia coli RNA polymerase (RNAP) and its auxiliary transcription factors have long been studied as a model for understanding regulation of elongation, but biochemical insights into some of …
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Unraveling the multifunctionality of human Spindly
… of the complex, involved in enhancing its processivity.<br/><br/>Previous reports have shown that the constant presence of Spindly at kinetochore impedes SAC inhibition, whereas its depletion allows for an alternative mechanism dynein- independent to silence the SAC on aligned kinetochores. …
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Experimental and theoretical investigation of mechanism of Kinesin motility
… force velocity relationship, stall forces, processivity and step size of kinesin and mutants targeting cover strand region. We show that A9G and D11G mutants move slower and have lower stall force then the wild type molecule, but the mutants are ultraprocessive, make steps of 7nm and have a …
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From the End to the Middle: Regulation of Telomere Length and Kinetochore Assembly by the RNR Inhibitor SML1
… and even shows low levels of repeat addition processivity. The correlation between telomerase activity and dGTP is conserved in human telomerase, which shows increased repeat addition processivity at increased dGTP concentrations. Thus, telomere length homeostasis is also sensitive to dNTP …
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The characterisation of family-13 kinesins in Trypanosoma brucei
… unlike most kinesins, do not exhibit microtubule processivity and are capable of depolymerising microtubules. They play important roles in bipolar spindle assembly and chromosome segregation. Of the six T. brucei Kinesin-13 proteins that were characterised during this study, only one was found to …
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Structural Basis for Coordination in Dimeric Kinesin
… falling off the track. A key requirement for processivity is that the nucleotide cycles of the heads are coordinated to prevent simultaneous release of both heads from the track. The structural basis for coordination has not been established yet. Here, we show the conformational changes …
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Regulation of DNA replication and cellular responses to perturbations in replication in the bacterium Bacillus subtilis
… component of the DNA replication machinery, processivity u-clamp, regulates the initiation of replication. This regulation appears to affect the loading of helicase, a replication component that generates the single-strand DNA template for replication. My results indicate that the replication …
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The Role of ORF59 and PAN ENE in Viral Replication of KSHV
… One of these factors is the viral polymerase processivity factor ORF59. The main functions of ORF59 are to localize the viral polymerase to the nucleus and to aid in polymerase processivity during viral DNA synthesis. Recently, ORF59 has been described to be a multifunctional protein. These …
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Solution structure and biochemistry of an intramembrane aspartyl protease (IAP)
… influence the catalytic efficiency but not the processivity and specificity of cleavages. Both positional and chemical variables were found to control the substrate specificity of mIAP. Small-angle neutron scattering (SANS) study showed that mIAP is a compact monomer in solution. Future …
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Studies of Huntington's disease associated motor domain phosphorylation of kinesin-1
… We found no significant difference in the processivity or ATPase activity of a phosphomimetic S175D construct or the non-phosphorylatable S175A construct. However, we did find that addition of a negative charge at S175 through phosphorylation or mutation led to a decreased stall force. …
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Mutational studies in the dNTP binding pocket of human immunodeficiency virus type 1 reverse transcriptase
… seems to be at the substrate binding step as the processivity of these mutants was not affected while the extent of primer utilization correlated with the loss of their polymerase activity. Curiously, these mutant derivatives of Q91 were found to be highly resistant to ddNTPs and exhibited greater …
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[RNA polymerase ribozymes]
… processive, with the polymerase achieving processivity as high as 90% in a few instances. The polymerase is currently limited by its low affinity for the primer-template. Future work will focus on improving primer- template binding, in order to produce a polymerase that can synthesize …
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