Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 58 for “"processive"”.
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Studying Processive Molecular Motors Inside Live Cells
Processive molecular motors are the proteins that transport various kinds of cargos within a cell. All processive motors have two motor domains that let the protein proceed by taking steps. These motor proteins can carry their cargos for microns by taking hundreds of steps before detaching form the …
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Processive bio-hybrid catalysts based on the T4 clamp protein
Contains fulltext : 72127.pdf (Publisher’s version ) (Open Access)
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Processive Acceleration of Actin Barbed End Assembly by N-WASP
… containing one WH2 domain does not generate processive bundles or filament loops leading us to believe that tandem WH2 is required for processivity. We propose that this novel N-WASP assembly activity provides an Arp2/3-independent force that drives nascent filament bundles into the basement …
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Fluorescence Imaging With One Nanometer Accuracy: Determination of How Processive Motors Walk
… transportation within a cell is accomplished by processive molecular motors. Motor proteins haul the cargo and transport it over long distances by taking hundreds of consecutive steps without dissociating from the cytoskeleton. Myosin V, myosin VI, and kinesin are well known processive motors. …
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Toward Creaturely Value: God's Inner Processive Life and the Intrinsic Value of Creatures in Thomas Aquinas
The intrinsic value of creatures is defensible on many accounts, though more rarely from a speculative, metaphysical, and theological perspective. In this thesis, I seek to establish and elaborate the connection between creaturely value and scholastic theology through the work of Thomas Aquinas. In …
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Biochemical and Microscopic Characterization of INFT-1: an Inverted Formin in C. elegans
… which promotes actin filament assembly while processively moving along the polymerizing filament barbed end. INFT-1 is one of six formin family members present in Caenorhabditis elegans (Hunt-Newbury et al., 2007) and is most closely related to vertebrate INF2, an inverted formin with …
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RNA replication by poliovirus RNA-dependent RNA polymerase: effects of residue 5 on elongation complex stability and processivity
… go through multiple steps of initiation before processive replication can happen. During primer-dependent initiation, the polymerase must bind the primer/template RNA substrate and undergo a conformational change to form a stable RNA-polymerase complex. After the stable RNA-polymerase complex is …
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Structural Insights into Mycobacteriales Galactan Biosynthesis
… 2 (GlfT2). While GlfT2 has been established as a processive polymerase with intrinsic sequence control, the mechanism underlying this activity remains unclear. In the studies presented here, we provide structural insights into Nocardia brasiliensis GlfT2 (NbrGlfT2) using X-ray crystallography and …
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INVESTIGATING THE BASES OF THE SPECIFICITY OF THE RESTRICTOR COMPLEX FOR THE TERMINATION OF NONCODING TRANSCRIPTION
… and the RNA-binding protein ZC3H4, suppresses processive transcription of thousands of noncoding RNAs (ncRNA) in mammalian genomes. The mechanism of Restrictor-mediated termination is not known but does not involve cleavage of the nascent RNA, setting it apart from known mammalian termination …
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High-Yield Cellulosic Hydrogen Production by Cell-Free Synthetic Cascade Enzymes: Minimal Bacterial Cellulase Cocktail and Thermostable Polyphosphate Glucokinase
… family 9 Clostridium phytofermentans processive endoglucanase, and family 48 Clostridium phytofermentans cellobiohydrolase was investigated on microcrystalline cellulose (Avicel) and regenerated amorphous cellulose (RAC). The optimal ratios for maximum cellulose digestibility were …
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Direct observation of XPD helicase base-pair stepping and regulation by RPA2
… duplex DNA in single base-pair steps, yet is non-processive, unwinding for short distances (~12 bp) and displaying a strong dependence on DNA sequence. Second, we investigated how RPA2 by itself interacts with DNA. We show that RPA2 can unwind duplex DNA in steps of ~5-8 bp in the presence of an …
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The identification and characterization of the interaction between Upf1 and PAB1 during nonsense-mediated decay & the identification of novel protein complexes associated with translation termination factor eRF1
… is required for a shift from distributive to processive deadenylation, but is not required for decapping or general decay during NMD. These results also revealed that this interaction plays a role in the normal shift in deadenylation mode for non-PTC containing mRNAs from the relatively slow …
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The Molecular Mechanism of Break Induced Replication
… into a homologous template followed by processive replication of DNA from the donor molecule. BIR is a key cellular process and is implicated in the restart of collapsed replication forks and several chromosomal instabilities. Recently, our lab demonstrated that the fidelity of DNA …
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Engineered Cytoskeletal Arrays Reveal Mechanisms Of Membrane Transport And Tubulation
… approach to investigate the interplay between a processive, microtubule-based motor, kinesin-1, and a non-processive, actin filament-based motor, Myo1c, in a simplified environment with increasing physiological complexity. First, we examined the interplay between purified motors attached to a …
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Genetic and biochemical analysis of a conserved DEAD-box helicase motif
DEAD-box proteins are non-processive helicases that use ATP to power unwinding of short RNA duplexes in essential steps of RNA metabolism. Despite acting on a diverse range of RNA substrates, DEAD-box proteins share a helicase core defined by 13 highly conserved amino acid motifs. Here we use S. …
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A Comparative Study of the Substrate Specificities of Peptidases A, B, and N From Salmonella Typhimurium
… the peptides. Neither PepA, PepB, nor PepN are processive in hydrolyzing their substrates. We have demonstrated the cooperation of PepA, PepB, and PepN in hydrolyzing some of their substrates.
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Structural Basis for Coordination in Dimeric Kinesin
… of Kinesin-1 are linked to function as a highly processive "molecular walker'' that can take hundreds of steps before falling off the track. A key requirement for processivity is that the nucleotide cycles of the heads are coordinated to prevent simultaneous release of both heads from the track. …
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Advancing Technologies for Precision Medicine: From Liquid Biopsy to Single-Molecule DNA Sequencing
… (RPS). This nanopore sensor requires a highly processive exonuclease enzyme to sequentially cleave nucleotides for detection. Therefore, enzymatic activity of lambda exonuclease (λ-exo) was characterized in both solution and solid phase to determine the reaction conditions compatible with …
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Engineering exclusively-quadruplet codon translation in vivo
… an 10-amino acid all quadruplet codon code and processive quadruplet codon translation of a small peptide within a standard bacterial chassis, without the need for genome recoding.
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Comparative modeling of mainly-beta proteins by profile wrapping
… that have al initiation stage' followed by processive, interaction between the unfolded region and the already-formed substructure. This wrap is then placed on a known structure and side-chains are modeled to produce a three-dimensional structure prediction. We demonstrate that wrapping onto …
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