Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 19 of 19 for “"processing bodies"”.
-
The Discovery of p62-dependent Processing Bodies
… phase separation <br/>(LLPS) to form p62 bodies to mediate the autophagic degradation of ubiquitinated proteins. <br/>At the time of this research, it was unknown whether p62 could exist in a differential droplet <br/>form to p62 bodies. Ribonucleoprotein (RNP) granules such as stress …
-
Investigating the regulation of processing bodies in Drosophila melanogaster oocytes
… and degradation. Within this category, processing bodies (P bodies) are evolutionarily conserved RNP granules found in yeast to humans. In Drosophila melanogaster, P bodies play a crucial role in regulating the maternally deposited axis-determining mRNA, bicoid (bcd). In the mature …
-
Investigating the function and mechanistic control of processing bodies in early Drosophila melanogaster development
… the function and mechanistic control of processing bodies (P-bodies), an evolutionarily conserved RNP granule. As perceived sites of RNA metabolism, the function of P-bodies, if any, has been widely debated. I investigate P-bodies, observing and manipulating their relationship with …
-
Novel Functions for Neuronal RNA Processing Bodies in the Control of Axon Terminal Growth in Drosophila Melanogaster
… functions for HPat/Pat1, a core component of RNA processing bodies or "P bodies". We show that <em>hpat</em> mutants exhibit a strong synaptic hyperplasia at the developing and acutely stimulated <em>Drosophila</em> larval neuromuscular junctions (NMJs). The synaptic defects observed in hpat …
-
Relationship between Processing Body Formation, Epithelial-to-Mesenchymal Transition, and Invasion in Lung Adenocarcinoma
… contexts, TGFβ1 enhances the formation of processing-bodies (P-bodies) where P-body proteins are required for invasion in multiple cancerous cell lines. P-bodies are a type of ribonucleoprotein (RNP) granule that assist in post-transcriptional gene regulation by storing or degrading mRNAs. …
-
Investigating processing body condensation, material properties and function during early Drosophila development
… phase separation. Conserved across eukaryotes, Processing bodies (P bodies) are cytoplasmic biomolecular condensates which act as hubs for RNA regulation including storage, translation, and degradation. During Drosophila oogenesis, several maternal mRNAs are stored and translationally repressed …
-
Interactions between Cucumber mosaic virus proteins and host proteins
… 2b protein molecules and sequestering them in processing-bodies, the 1a protein decreases the proportion of 2b protein molecules available for binding AGO1. This ameliorates 2b-induced viral symptoms and moderates the induction of aphid resistance. However, the 1a-2b protein interaction does …
-
Functions of Deadenylation Factors In Mrna Decay and Mrna Processing Body Formation
… degraded in dynamic cytoplasmic foci termed mRNA processing bodies, or P bodies, which are conserved in eukaryotes. To shed new light on the mechanisms of P body formation and P body functions, I focused on the link between deadenylation factors and P bodies. I found that the two major …
-
Regulation of exogenous retroviruses and endogenous retroelements by MOV10
… all of which colocalise in cytoplasmic mRNA processing bodies and stress granules. Considering MOV10 cellular associations and homolog functions, the capacity of MOV10 to regulate the replication of a diverse panel of genetically distinct retroelements was investigated here.<br/>Ectopically …
-
Investigating the calcium wave and actin dynamics at Drosophila egg activation
… for imaging. Through visualisation of calcium, Processing bodies and meiotic spindles, I show that osmotic pressure acts as an initiation cue for the calcium wave and downstream processes, including the resumption of cell cycle and the dispersion of the translational repression sites. I further …
-
Licensing Of Cell Fate Reprogramming Via Leafy Transcription Factor Pioneering Activity
… Additionally, these droplets co-localize with processing bodies and co-expression of LFY co-regulator drove even bigger puncta. Our data suggested that droplet formation might be a regulatory mechanism to prevent nuclear entry of LFY when not needed and that proteasome mediated degradation …
-
Mechanisms of persistent translation arrest following global brain ischemia and reperfusion
… of global brain I/R using <i>in situ</i> antibodies and mRNA staining methods, <i>in viv0</i> protein synthesis and Western blots. Results are collectively summarized below:</p> <p>1. Cytoplasmic poly(A) containing mRNAs redistributed to form mRNA granules in reperfused neurons.</p> <p>2. mRNA …
-
Regulation of APOBEC3 activity and HIV-1 replication by P-body associated proteins
… to discrete cytoplasmic foci termed mRNA Processing (P) Bodies. These foci have very recently been implicated in influencing viral life cycles. However, the functional relevance of the interaction with the Argonaute proteins and localisation to P-bodies, to APOBEC3 anti-viral and cellular …
-
Quantitative Studies of Composition and Formation of Yeast P Bodies
… protein partitioning into condensates. Using P bodies, an archetypal biomolecular condensate that concentrate proteins and RNA, I first generated a quantitative inventory of the major proteins in yeast P bodies. I found that only 7 proteins are highly concentrated in P bodies while the 24 others …
-
Stress-Induced Targeting of Molecular Chaperones In The Yeast Saccharomyces Cerevisiae
… Ssa1, and the sHSP Hsp26 accumulate into RNA processing bodies (P- bodies) and stress granules, sites of mRNA metabolism. I found that Hsp104 recruits both Ssa1 and Hsp26 to P-bodies and that these three chaperones are required for stress granule formation. These findings suggest a possible …
-
Characterisation of the multi-isomeric protein nesprin-1 in p-body and mRNA dynamics
… promoter usage and alternative 3’ end processing, the nesprins are able to generate multiple mRNA transcripts, leading to the production of diverse tissue specific isoforms with potential roles beyond the NE. To explore further the capacity of nesprin-1 to generate alternative …
-
SYK promotes TGF-beta-induced P-body clearance in breast cancer cells through the enhancement of autophagy
… A number of these mRNPs are associated with processing bodies (P-bodies), mRNP granules that are associated with mRNA degradation. These interactions led me to hypothesize that Syk co-localizes with P-bodies. I found that Syk co-localizes with P-bodies in HEK293T cells. Moreover, I show …
-
Characterising FAM117 proteins as novel targets of DYRK1B
… DYRK1B function, in this case the promotion of processing body formation. From observing FAM117 localisation I also show that they partially co-localise with cytoskeletal proteins, such as tubulin, and show strong co-localisation with focal adhesion proteins. FAM117 proteins are reported to be …
-
The Regulation of ARE-Mediated mRNA Decay During Colorectal Tumorigenesis
… occurs in cytoplasmic RNA decay centers known as processing bodies (P-bodies). However, the mechanism that drives P-body assembly is largely understudied. The mRNA decay factor tristetraprolin (TTP), by virtue of its ability to bind AREs, promotes ARE-mediated mRNA decay by sequestering its target …