Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"prions"”.
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Prions Propagate as Dynamic Strain Mixtures
Prions are the agent responsible for a group of transmissible and inevitably fatal neurodegenerative disease in humans and other mammals. Prion disease is caused by the misfolding of a host encoded protein, PrPC, into the infectious conformation PrPSc. Phenotypic differences in disease (i.e., …
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Structural Elucidation of [RNQ+] prions Transmissible Infectivity
… found to be the result of aggregated proteins or prions present in the brain. Prions are obtained by one of these three means: familial, acquired, or sporadic; with the majority of cases found in nature being sporadic. A variety of organisms posses proteins that are capable of undergoing …
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Discovery and characterization of prions in Saccharomyces cerevisiae
… reaction. These aggregates, or prions, are the infectious agents behind diseases like Kuru and mad-cow disease. In yeast, however, prions act as epigenetic elements that confer heritable alternative phenotypes. Prion-forming proteins create bistable molecular systems whose …
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Metabolic Profiling of Prions In The Gastro-Intestinal Tract
<p><strong>Metabolic profiling of prions in the gastro-intestinal tract</strong><strong></strong></p> <p><strong>Uffaf Khan, B.S.</strong></p> <p><strong>Thesis Advisor: Claudio Soto, Ph.D.</strong></p> <p><strong>Abstract</strong></p> <p>Prion diseases, also known as transmissible spongiform …
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Single Molecule Fluorescence Studies of Prions and Prion-like Proteins
Prions are infectious agents that cause fatal neurodegenerative diseases in the brain. The wide-accepted protein-only hypothesis states that the misfolded form of prion protein (PrP) is the sole constituent of prions, and the self-propagating process of PrP is considered to play a central role in …
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Biochemical, computer, and spectroscopic techniques applied to the study of prions and of combinations of antineoplastic drugs
This thesis collects the work I have done during the three-year PhD Course. The results obtained are divided according to the research topics addressed: · Drug discovery of anticancer agents and development of synergistic associations (Part I); · Studies on the prion structure and the pathogenesis …
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Decontamination of prions, prion-associated amyloid and inefectivity from surgical stainless steel - implications for the risk of iatrogenic transmission of CJD
… impact on the risk of iatrogenic transmission of prions has been<br/>investigated. Critically, the use of a fluorescent amyloid fluorophore for the detection of prionassociated<br/>amyloid as a marker for disease permitted the investigation of the role of amyloid<br/>in infectious disease under …
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Prion biology in the context of bacteria
Prions are infectious amyloid aggregates first described in the context of mammalian neurodegenerative diseases collectively known as the transmissible spongiform encephalopathies. Prions have also been uncovered in yeast, where they function as protein-based units of heredity that confer unique …
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Use of the Protein Misfolding Cyclic Amplification for food safety and drug discovery
… disease (CWD) remains uncertain, the presence of prions in food products raises public health concerns. Additionally, therapeutic strategies for prion diseases remain limited. This thesis presents a methodological exploration of the Protein Misfolding Cyclic Amplification (PMCA) technique to …
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Development of A High-Throughput System For Screening of Anti-Prion Molecules
… humans and animals. As other infectious agents, prions display strain variation, which can generate different pathological outcomes in affected individuals. Unfortunately, there are no known therapies for these diseases, which at present are invariably fatal. In this work, the Protein Misfolding …
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Mutational analysis of Hsp110 suggests an integral role for the chaperone in yeast prion propagation.
Prions are highly aggregated infectious proteins that have been identified in mammals and in some fungal species. The discovery that prions exist in the yeast species S. cerevisiae has enabled researchers to explore the characteristics of these unusual proteins and how they propagate within the …
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[GAR⁺] : a novel type of prion involved in glucose signaling and environmental sensing in S. cerevisiae
Several well-characterized fungal proteins act as prions, proteins capable of multiple conformations, each with different activities, at least one of which is selfpropagating. We report a protein-based heritable element that confers resistance to glucosamine, [GAR⁺]. Genetically it resembles other …
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Assessing the role of Hsp70 in prion propagation in Saccharomyces cerevisiae
… was first described by Stanley Prusiner in 1982. Prions are infectious proteins and are responsible for many neurodegenerative diseases, collectively termed as transmissible spongioform encephalopathies, including; BSE, vBSE, scrapie and CJD. Prions are also present in fungi. There have been a …
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Development of the new yeast-based assays for prion properties
… that the ability to form transmissible amyloids (prions) is widespread among yeast proteins and is likely intrinsic to proteins from other organisms. However, the distribution of yeast prions in natural conditions is not yet clear, which prevents us from understanding the relationship between …
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Molecular Basis of Mammalian Prion Protein Misfolding
Prions are aberrantly folded proteins that are able to self-propagate their abnormal conformation using the normally folded protein as substrate. In mammals, the only known prion protein is PrP. The misfolding of PrP is a key event underlying Transmissible Spongiform Encephalopaties (TSEs), fatal …
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A genetic platform for the study of protein perturbation and prion-based inheritance
… or degradation. I applied yTRAP to track yeast prions, which have previously been difficult to study due to a lack of simple and reliable reporters. Prions are protein-based elements of inheritance that have profound implications for the evolution of single-celled organisms. I first utilized …
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Soil PH and clay content associated with chronic wasting disease in white-tailed deer in northern Illinois
… and CEC appear to alter the availability of prions immobilized in soil. The results suggest that exposure to prions through probable routes of infection such as inhalation or ingestion is greatest where pH is greater than 6.6 and the percent clay is less than 20%.
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Mielių baltymo Sup35 GNNQQNY sekos mutantinių variantų kūrimas ir jų sintezė Saccharomyces cerevisiae ląstelėse /
Prions are proteinaceous particles that are able to replicate and spread as an infection. Prions cause transmissible spongiform encephalopathies (TSEs), a fatal neurodegenerative disease in mammals. Neurodegenerative diseases also include amyloidoses, better known as Alzheimer's, Parkinson's or …
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Investigating genetic vulnerability to chronic wasting disease in endangered cervid taxa
… progressive neurodegenerative diseases caused by prions. Prions are misfolded infectious proteins with a stable altered structure that allows them to induce conformational changes in normal proteins and make them infectious prions. As the infection spreads, it causes irreversible damage primarily …
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Comparative ecology of Pachyptila species breeding sympatrically at Gough Island
Prions (Pachyptila spp.) are one of the most abundant seabird groups of the Southern Ocean but their taxonomy, at-sea distribution and foraging ecology are poorly known. There has been considerable confusion surrounding the taxonomy of prions and their identification at sea is problematic. Recent …
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