Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"primitive streak"”.
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The Role and Control of WNT Signalling in an HESC Model of Human Primitive Streak
… gastrulation is marked by the creation of the primitive streak (PS) and is largely controlled by WNT, BMP, and ACTIVIN/NODAL signalling. Despite detailed characterization in model organisms, the human PS and the role these pathways play in its formation and patterning remains a mystery. In this …
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Automatic Cell Tracking For Studying Large Epithelial Sheets Of Cells And Its Application To Chicken Gastrulation
… event of the gastrulation is formation of the primitive streak that is characterised by large scale vortical flows that involve an order of >200,000 cells. During this self-organised process individual epithelial cells are migrating in a highly coordinated manner. The cell behaviours driving …
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Investigation of cell movement and the associated cytoskeleton during chick gastrulation and somitogenesis
… avian gastrulation, cells ingress through the primitive streak. Previous characterisation of microtubule organisation during this process revealed the distribution of cells with polarised and radial arrays across different regions of the embryo. Interestingly, many cells organised into groups …
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The effects of BMP4 on different in vitro models for mouse gastrulation and neural induction
… transition (EMT) that gives rise to the Primitive Streak. BMP4 mutant mice fail to form a Primitive Streak or do so defectively. Neural induction is the process that gives rise to the brain, by ensuring that embryonic cells become neural tissue rather than mesoderm, endoderm, or other …
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Role of multicellular organisation in mesoderm differentiation
… to mesenchymal transition (EMT), forming the primitive streak. This is accompanied by a number of changes in tissue architecture including the loss of apical-basal polarity and cell-cell adhesion, an increase in cell clustering and migratory behaviour, and changes in cell shape associated with …
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Pluripotency and patterning in nonhuman primate embryogenesis
… 3D expression patterns before and after primitive streak formation. Spatial transcriptome analysis revealed that pluripotency is sustained to the anterior EmDisc, in contrast to the mouse. Integration of RNA-seq datasets of marmoset preimplantation embryos generated a blueprint from …
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Eomes Directs the Formation of Spatially and Functionally Diverse Extra-Embryonic Mesoderm Derived Tissues
… mesodermal cells to originate at the posterior primitive streak will give rise to the extra-embryonic mesoderm (ExEM). These cells will contribute to key tissues supporting the development of the embryo, including the amnion, chorion, yolk-sac, and allantois. Together, they protect the embryo …
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Characterizing the Immunogenicity of Islet-like Cells derived from HLA-edited Human Pluripotent Stem Cells
… into cells bearing the successive phenotypes of primitive streak, definitive endoderm, and pancreatic progenitors. I next explore mechanisms that regulate the endocrine composition of human pluripotent stem cell-derived islets, finding that events both prior to and concurrent with endocrine …
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A new computational model for multi-cellular biological systems
… two proposed hypotheses for the formation of the primitive streak in the chick embryo are investigated and compared. These applications demonstrate how the model can be used to complement experimental studies and help to tease out the mechanical processes driving morphogenesis.
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Mechanism of Lmx1b-regulated Limb Dorsalization
… was electroporated into chick embryos at the primitive streak stage (Hamburger-Hamilton (HH) 4) and during early neural tube formation (HH10). GFP expression was detected in neural tube and surrounding the developing midbrain and cerebellum. Expression of Lmx1b and KLD mRNA overlaps the …
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The Role of Snail Homolog 1 in Embryonic Stem Cell Differentiation
… transition: EMT) as they ingress through the primitive streak and form mesoderm. To better understand the molecular pathways of EMT during this developmental transition, we developed a model system utilizing mouse embryonic stem: ES) cells. We show that EMT occurs during ES cell …
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Investigations of collective cell movement during gastrulation in the chick embryo
… in a collective manner. They ingress through the primitive streak and follow routes that reflect their developmental fate. Despite its importance for the formation of the body plan, the mechanisms that define this process remain elusive. Previously, the introduction of external sources of growth …
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Redefining the Gastrula Organiser in Early Chick Embryo Development
… a gradient from anterior to posterior along the primitive streak (PS). Grafting sections of the PS from different anteroposterior (AP) levels into host embryos showed that neural induction ability is not confined to the node, but is also distributed as an AP gradient along the PS. An …
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Morphogenesis of the early post-implantation mouse embryo
… a potential role for these perforations in primitive streak formation and extension. Together, my studies bring new important insights into the understanding of early mouse embryo morphogenesis.
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An in vitro model of human yolk sac and epiblast crosstalk
… endoderm, plays a crucial role in restricting primitive streak formation to the posterior epiblast, thereby establishing the anterior-posterior axis of the mouse embryo. Recent studies of human embryos cultured to postimplantation stages have alluded to the presence of a human equivalent of the …
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Nodal organized cell dynamics for building tissue shapes in vertebrate gastrulation
… beads at the area pellucida edge away from primitive streak in chick embryos during late HH2 or HH3 stages. I found that cells exposed to Nodal cluster along the dorsal-ventral axis and show ectopic Brachyury (a mesoderm marker) expression, and some treated embryos show a secondary body …
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Developing a stem-cell system of embryonic development organised by a morphogen signaling centre
… confirmed the localization of brachyury, a primitive streak marker in some gastruloids. Collectively, the results show that the gastruloids have the capability to undergo self-organization in a spatially coordinated manner and that bone morphogenetic protein-4 could replace the Chiron pulse …
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Multi-scalar mechanisms timing axial progenitor contribution to the vertebrate body axis
… progenitors undergo ingression through the primitive streak but are delayed relative to HH4 progenitors at the transition from ingressed to migratory mesenchyme, ultimately resulting in a more posterior axis contribution. To assess the role of Hox gene expression, I identified a set of genes …
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Spatial Transcriptome Profiling of Gastrulating Primate Embryos
… in primates. I analysed the formation of the primitive streak, a structure which defines the embryo midline in the posterior of the embryo proper and is established following the onset of gastrulation. I investigated signalling pathways which I extrapolated from mouse and primate literature …
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