Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"primer walking"”.
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Novel molecular engineering approaches for genotyping and DNA sequencing
… short read-length of SBS. We developed a novel primer walking strategy to increase the read-length of SBS with cleavable fluorescent nucleotide reversible terminators (CF-NRTs) and nucleotide reversible terminators (NRTs) or hybrid-SBS with cleavable fluorescent nucleotide permanent terminators …
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Characterization of Helicobacter pylori sRNAs HPnc2525, HPnc2600, and HPnc2645
… HPnc2600, and HPnc2545. I devised an RT-PCR primer walking strategy to delimit the 5' end of HPnc2525 further. I analyzed the sRNA sequences defined by this and previous research using the TargetRNA2 program and predicted these sRNAs regulate virulence (e.g., cagL, hopZ, and fliA) and other …
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Sequenziamento e Analisi Molecolare di Varianti Alleliche del Gene PRB1
The aim of this work was the molecular characterization of gene PRB1 in three subjects with different salivary proteome profile. The PRB1 gene encodes a member of the heterogeneous family of Basic Proline rich Proteins (PRB), produced by the parotid gland and secreted in human saliva. The protein …
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Mechanism of Pathogenesis and Replication of an Avian Strain of the Hepatitis E Virus in a Chicken Model
… strain of avian HEV was determined using primer walking strategy. The full-length genome of the avirulent strain is 6649 nts in length and has a nucleotide sequence identity of 90.1% with the prototype pathogenic strain. Numerous non-silent mutations were observed in ORF1, the region …