Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"primer design"”.
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Thermodynamics of λ-PCR Primer Design and Effective Ribosome Binding Sites
… process is minimal, the importance of accurate primer design cannot be overstated. The target of this study was to create an effective procedure for λ-PCR primer design that ensures accurate cloning reactions. Additionally, synthetic ribosome binding sites (RBS) were included in the primer …
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A systematic and extensible approach to DNA primer design for whole gene synthesis
… is not rapidly progressing because most gene design software takes an excessively naive approach to the complex problem of designing component oligonucleotides for whole gene synthesis. The synthetic biology community needs a flexible, robust and optimal primer design tool. We present the …
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MULTIPLEX ASSAY DESIGN
This thesis explores the design of assays for the multiplexed detection of various infectious diseases. A single-pot, 8-plex end-point PCR reaction for the detection of carbapenemase-producing Enterobacteriaceae (CPE), vancomycin-resistant Enterococcus (VRE) and colistin resistance has been …
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Characterization of Novel Hantaviruses in Paraguay
… an additional research approach to accelerate primer design so as to obtain a full reference genome of novel orthohantaviruses. In these studies, I used both vRNA and cRNA primers to amplify the segment from rodent tissue and identify an initial reference genome which is presumably composed of …
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Investigating the functionality of the transmembranes in GusB by fusing it with LacY using the polymerase chain reaction.
… Initially, the fusion was successful until a primer design error became evident during restriction digest. This resulted in the primers being redesigned and the PCR was repeated. However, obtaining the fusion since the correction proved difficult and required numerous troubleshoots in which …
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Discovery of a Novel Microalgal Strain Scenedesmus Sp. A6 and Exploration of Its Potential as a Microbial Cell Factory
… cost of less than $800 USD. Finally, a new primer design tool for the ligation-independant cloning technique 𝜆-PCR was developed called lambdaPrimeR. The contributions of this work are the discovery and development of different tools that can overcome the challenges of the use of microalgae …
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Post-mortem Molecular Investigation: exploring genetic variation in CYP2D6 in deceased individuals at Salt River Mortuary
… a local context. The aim of this project was to design and optimise a molecular-based assay to sequence the drug metabolising enzyme, CYP2D6. Subsequent to primer design, exons in CYP2D6 were amplified and sequenced. The optimised assay was then applied to DNA from two decedents suspected to have …
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High sensitivity pathogen detection assays from crude samples
… swabs and saliva samples. Starting with primer design, we developed highly sensitive RT-LAMP based assays that can detect low copies of SARS-CoV-2 pathogen. We challenged ourselves to demonstrate even single-molecule detection of SARS-CoV-2 using a modified 2-step RT-LAMP process where …
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DNA–based data storage system
… For this purpose, WMU sequences used for primer design in DNAbased data storage systems are also required to be at large mutual Hamming distance from each other, have balanced compositions of symbols, and avoid primer-dimer byproducts. We derive bounds on the size of WMU and various …
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Hubungan kekerabatan beberapa kultivar pisang (Musa sp.) untuk sifat ketahanan terhadap penyakit berdasarkan Resistance Gene Analog (RGA)
… pada RGA dapat digunakan sebagai dasar pembuatan primer, sehingga terdapat perbedaan pita amplifikasi antara kultivar tahan dan rentan. Oleh karena itu penelitian ini bertujuan untuk mengetahui hubungan kekerabatan beberapa kultivar pisang (Musa sp.) untuk sifat ketahanan terhadap penyakit …