Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 77 for “"primary cultures"”.
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A feasibility study of chemosensitivity testing in primary cultures from malignant gliomas, and analysis of drug resistance mechanisms
Malignant gliomas are the commonest primary malignant tumours of the brain and carry a poor prognosis with current treatment modalities. Chemotherapy is an increasingly important treatment option for these turnouts; however response is limited by the development of drug resistance. A study was …
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Phenotype-dependent regulation of the system x<sub>C</sub>- cystine/glutamate exchanger by glutathione levels in rat astrocyte primary cultures
… was investigated in cultures of primary astrocytes prepared from neonatal rats. We report that Sx<sub>C</sub><super>-</super> activity in the dbcAMP-treated cells was nearly 7-fold greater than in untreated astrocytes (100 ± 21 and 15 ± 4 pmol/min/mg protein …
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Phenotype-dependent regulation of the system x<sub>C</sub>- cystine/glutamate exchanger by glutathione levels in rat astrocyte primary cultures
… was investigated in cultures of primary astrocytes prepared from neonatal rats. We report that Sx<sub>C</sub><super>-</super> activity in the dbcAMP-treated cells was nearly 7-fold greater than in untreated astrocytes (100 ± 21 and 15 ± 4 pmol/min/mg protein …
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Growth and Characterization of Cell Cultures of Rainbow Trout
Gonad and spleen primary cultures of rainbow trout (Salmo gairdneri Richardson) were initiated and compared to the rainbow trout gonad transformed cell line RTG-2. The RTG-2 cell line grew faster than the gonad primary cultures. The chromosome number of the gonad primary culture was 2n=56 with 104 …
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Organ and primary culture of medaka (Oryzias latipes) testis: Test systems for the analysis of cell proliferation and differentiation
… from spermatocytes were viable and motile. Primary cultures were characterized over a period of two days with respect to cell viability and the distribution of adherent and suspended cells. These two cell populations were maintained in dynamic equilibrium in vitro for several days. …
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Biological Potencies and Mechanism of Action of Estrogens and Antiestrogens in Primary Cell Cultures of Human Endometrium and in Mcf-7 Human Breast Carcinoma Cells in Vitro
Primary cultures of human endometrial cells were used to examine the direct effects of estrogens on progesterone receptor levels in vitro. Cells, grown in Dulbecco's EMEM, showed maximal stimulation of progesterone receptor (PR, 7-fold) after a 3-4 d incubation with 10nM estradiol (E(,2)). The
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The Role of Amino Acids and the Threonine-SAM Pathway in the Development of Bovine Inner Cell Mass and Pluripotency
… day old (D6) ICM outgrowths but absent in D6 TE primary cultures. In bovine embryos, Qc1 reduced blastocyst grade 1-3 (B¹⁻³) and B¹⁻² embryo development 1.5- and 2-fold, respectively, compared to the dimethyl sulfoxide (DMSO) controls. Qc1 also reduced ICM and TE cell numbers ~2-fold. This cell …
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Regulations and functions of P2Y₂ and P2X₇ nucleotide receptors in the central nervous system
… of P2X and P2Y nucleotide receptors in rat primary cultures of cortical neurons (rPCNs), in adult rodent brains, in cerebral ischemia and in collared rabbit carotid arteries. P2Y₂R is up-regulated in response to cytokine stimulation in rPCNs in vitro and after collar transplantation around …
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Effect of cell- and species-differences in the expression of drug metabolism enzymes on chemical-induced injury to the rat and human kidney
… increased CH formation in kidney microsomes. Primary cultures of rPT and rDT cells maintained their expression of GSH-dependent enzymes but not P450 isoforms. Freshly isolated human proximal tubular (hPT) cells expresses CYP4A11, GSTA, GSTP, and GSTT but not GSTM. Tri and DCVC were toxic to …
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The role of apoptosis in <i>in vitro</i> and <i>in vivo</i> models of amyotrophic lateral sclerosis
… of thesis I first characterized apoptosis in primary cultures of motor neurons exposed to detrimental stimuli, and then I evaluated the possible involvement of apoptosis in the cervical spinal cord neurons of early symptomatic wobbler mice. <br></br><br></br>In primary cultures of motor …
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Endoplasmic Reticulum Dynamic Structural Changes in Neurons: The Fission-Fusion Phenomena
… Ca2+ entry to the cell in murine primary cultures and hippocampal slice cultures. Using different pharmacological approaches, we demonstrate, that ER fission is triggered independently on Ca2+ from ER stores. Subsequently, we show that mild hypothermia, reported to be protective in …
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Generation of nitric oxide and its protective and toxic actions in the gastrointestinal tract
… by hypertonic saline in vivo, (iii) to expose primary cultures of guinea-pig gastric mucosal cells to oxidative challenge and an NO donor, and to investigate the response in terms of heat shock protein 72 (HSP 72) induction, and (iv) to investigate the induction of iNOS and the role of …
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A novel bioengineering platform using functionalized self-assembly peptides to enhance CYP3A2 activity in modified rat hepatocyte sandwich cultures
… notoriously known to quickly lose expression in primary cultures, by creating a platform based on collagen sandwich cultures. The modified sandwich cultures are substituted with self-assembling peptide, RAD16-I, combined with integrin-binding sequence RGD or laminin receptor binding sequence …
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A Biochemical Study of Gossypol and Lactate Dehydrogenase X Binary Interactions
… isozyme, and (b) its binding in vitro in primary cultures of spermatogenic cells. Mouse LD-X was cleaved with trypsin before and after treatment with gossypol. This was followed by high performance chromatography (HPLC) separation of the LD-X tryptic peptide fragments to observed …
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Signal transduction pathways involved in skeletal muscle fibre type regulation
… calcineurin and ERKI/2 signalling pathways in primary cultures of rat myotubes. Differences in the levels of various signal transduction proteins/transcription factors between slow and fast muscle were investigated using western blotting. The nuclear translocation kinetics of NFAT and NF-icB in …
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Assessing The Therapeutic Viability of Hsc70 in ALS
… – Hsc70 – also promotes motor neuron survival in primary cultures. Unfortunately, the price of both commercially available proteins makes them cost prohibitive to study, thus acquiring them by different means is necessary for further investigation. Here we present a reliable protocol for the …
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Small molecule channels increase anion secretion and host defenses in cystic fibrosis airway epithelia: mechanistic studies and initial clinical translation
… key components of airway host defenses, in primary cultures of CF airway epithelia. AmBisome, a clinical AmB formulation safely used to treat and prevent pulmonary fungal infections in >1,600 people, increased ASL pH in four CFTR-/- pigs and increased ASL pH and ASL bacterial killing in …
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Neuroprotective Effects of Anthocyanins on Neuronal Death Induced by Inhibition of Bcl-2 and Oxidative Stress
… anthocyanins, against MOS-induced death in primary cultures of rat cerebellar granule neurons (CGNs). Anthocyanins are natural antioxidants whose neuroprotective potential has yet to be examined in detail. Kuromanin and callistephin are anthocyanins derived from black rice and strawberries, …
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The role of eicosanoids in the human skin's response to ultraviolet radiation.
… basal and UVR-stimulated conditions. In addition primary cultures of epidermal melanocytes (EM) were established from 16 individuals (8 SPT-1 & 8 SPT-4) and assessed for the presence of eicosanoid-producing enzymes, melanogenic enzymes and NO synthases, by immunocytochemistry, Polymerase Chain …
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Studying the Effect of Low Doses of Ionization Radiation on Senescence in Human Lung Fibroblasts.
… study the effect of LDR on senescence using primary cultures.
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