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Showing 1 to 20 of 39 for “"primary culture"”.
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Primary culture of human limbal-corneal epithelial cells under different conditions
… outgrowths from human cadaveric limbal explants cultured on cell culture inserts and denuded human amniotic membrane (dHAM) for properties associated with LSCs and to understand the human limbal stem-cell biology in vitro with and without 3T3 feeder cells. In particular, the expression of stem …
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A Pilot Study of Primary Culture of Oral Epithelium using the Direct Explant Technique
Purpose – This investigation was a pilot study to determine the feasibility of using the direct explant technique for tissue that is typically discarded from extracted teeth as a source for mesenchymal stem cells (MSCs). The long-term goal of this research is to identify an easily obtainable source …
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Primary Culture of Rodent Kidney Cells as a Model for Study of Sodium and Potassium Ion Transport Regulation
Made available in DSpace on 2014-12-14T13:39:42Z (GMT). No. of bitstreams: 1 7811207.pdf: 6217442 bytes, checksum: 3191556b609ed6114ef69c6bc1932a56 (MD5) Previous issue date: 1978
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Organ and primary culture of medaka (Oryzias latipes) testis: Test systems for the analysis of cell proliferation and differentiation
In cultured medaka testis fragments, cells remained viable for the entire culture period (17h), and spermatids that developed from spermatocytes were viable and motile. Primary cultures were characterized over a period of two days with respect to cell viability and the distribution of adherent and …
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Growth and Characterization of Cell Cultures of Rainbow Trout
Gonad and spleen primary cultures of rainbow trout (Salmo gairdneri Richardson) were initiated and compared to the rainbow trout gonad transformed cell line RTG-2. The RTG-2 cell line grew faster than the gonad primary cultures. The chromosome number of the gonad primary culture was 2n=56 with 104 …
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Insulin and TSH signal transduction pathways in human preadipocytes.
… pathway was examined in human preadipocytes in primary culture. The inhibitory effect of rapamycin on insulin-induced differentiation of preadipose cell lines has been attributed to a blockade of clonal expansion. We demonstrate that rapamycin inhibits the differentiation of human preadipocytes …
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In vitro screening of crude extracts and pure metabolites obtained from marine invertebrates for the treatment of breast cancer
… human breast epithelial cells (HBEC) in primary culture. Eight samples exhibited significant [drug concentration resulting in a 50% decrease in cell growth as compared withcontrols (ED50), < 25 gg/ml] dose-dependent cytotoxicity against the drug-sensitive cell lines; the ED50 values …
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Staphylococcus aureus의 Lipoteichoic acid(LTA)에 의한 사람의 정상 코 점막 상피세포에서의 MMP와 TIMP의 발현
… A. 사람의 정상 코 점막으로부터 분리한 상피세포 배양 1. 사람 코 점막 상피세포의 Primary culture 2. Air Liquid Interface-culture(ALI-culture) B. Lipoteichoic acid(LTA) 처리 C. MTT assay D. RNA 추출 및 cDNA 합성, PCR E. Enzyme-linked immunosorbent assay(ELISA) F. Gelatin zymography 1. Gelatinase B의 준비, 활성화 2. Gelatinase B의 전기영동, 기질-효소 간 …
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Interrelationships among estrogen, insulin-like growth factor-I and cyclic adenosine monophosphate in the regulation of uterine progesterone and estrogen receptors
… Exposure of uterine cells maintained in primary culture to estradiol, IGF-I, or to agents which raised intracellular cAMP levels stimulated significant increases in progesterone receptor (PR) content. Stimulation of PR levels by these agents was suppressed equally by co-treatment with …
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Opioid inhibition of the growth of the transplantable androgen-responsive (AR) Shionogi mouse mammary carcinoma (SC115)
… factor antibodies on the growth of cells in primary culture from the transplantable androgen-responsive(AR) Shionogi mouse mammary carcinoma (SC115). In addition, the effects of opioid agonists and antagonists on the growth of SC115tumor cells in primary culture were examined. The possible …
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Glucose-Induced Apoptosis of Schwann Cells In vitro
… emulating hyperglycemia stimulate apoptosis in cultured Schwann cells. Schwann cells were isolated from two-day-old rat pup sciatic nerves, placed in primary culture, and then incubated in the presence of elevated glucose levels for one to seven days. The glial marker S100 monoclonal antibody …
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Coordinated Control of Liver-Specific Genes in Cultured Cells from Mice and Rats
… liver-specific and common mRNAs in fresh livers, primary hepatocyte cultures, and hepatoma cell lines, derived from mice or rats. The transcription rates of many different liver-specific but no common mRNAs were found to decline sharply within 24 hours when hepatocytes from the liver were …
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Temperature Acclimation of Enzyme Activities and Protein Synthesis in Channel Catfish (Ictalurus Punctatus). In Vivo and In Vitro Studies
… perfusion were maintained for 3-4 weeks in primary culture at 7, 15, and 25(DEGREES)C on biomatrix prepared from liver structural proteins. Specific activities of enzymes of cultured hepatocytes showed acclimatory compensation for the following: cytochrome(DEGREES)C oxidase, citrate …
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Pharmacological studies of C. elegans cultured in axenic media: nicotine and its effects on growth, body morphology, and reproduction
… adaptation studies for decades. The primary culture method for raising C. elegans is to use bacterial Escherichia coli media plates. Using bacterial media can be resource intensive, result in turbidity for microscopy based assays, and bacterial metabolism can affect any …
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Expression and regulation of genes associated with CNS regeneration and plasticity
… 13 2. In vivo 5-aza and SAM administration 13 3. Primary culture of dissociated adult DRG neurons 14 4. Genomic DNA extraction for MeDIP-seq 14 5. Methylated DNA immunoprecipitation and sequencing 15 6. Bioinformatics analysis 15 7. Bisulfite sequencing 17 8. Chromatin immunoprecipitation assay 18 …
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The Voltage Gated Sodium Channel β1/β1B subunits: Emerging Therapeutic Targets in the Heart
… we were unable to translate them into a primary culture of cardiac cells with endogenous expression of β1 because we found that both neonatal rat cardiomyocytes and isolated adult mouse cardiomyocytes do not express β1 at detectable levels, whereas they do appear to express β1B. In …
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Intercultural conflict styles in the criminal justice system and the implications for intercultural interventions
… combined research strategies suggested that the primary culture of the criminal justice system was consistent with European American cultural preferences for low-context, direct, and emotionally restrained communication and conflict styles, which contrasts with the general preference of the …
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Molecular mechanisms mediating the induction of apoptosis by chemopreventive selenium compounds
… and human oral carcinoma cells (SCCs), using a primary culture system. Two selenium compounds were tested: selenodiglutathione (SDG), the primary metabolite of selenite, the most commonly used cancer-protective selenium compound in animal models, and the synthetic compound, 1,4-phenylenebis …
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Modelling the gastric epithelium for testing of new chemical entities
A cell culture model of the gastric epithelial cell surface would prove useful for biopharmaceutical screening of new chemical entities and dosage forms. A successful model should exhibit tight junction formation, maintenance of differentiation and polarity. Conditions for primary culture of …
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The involvement of the kynurenine pathway in the pathogenesis of multiple sclerosis
… mice, the most common animal model for MS and primary culture of human oligodendrocytes, we have studied the involvement of the KP in neuroinflammation [Indoleamine 2,3-dioxygenase (IDO-1) expression and its immune regulatory role] and neurodegeneration (balance between neuroprotective and …
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