Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"primary cortical neurons"”.
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An in vitro study to characterize a new automated high throughput neuronal stretch injury system
… investigation, a new methodology for culturing primary cortical neurons of rat origin was established. Viable cell cultures developed for the first time in NJIT laboratory were successfully injured using the new NJIT device. The data recorded here may allow us to understand better the complex …
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Astrocytes Rescue Neuronal Health After Cisplatin Treatment Through Mitochondrial Transfer.
… that astrocytes transfer healthy mitochondria to neurons after cisplatin treatment to restore neuronal health.</p> <p>We used an <em>in vitro</em> system in which astrocytes with Mito-mCherry-labeled mitochondria were co-cultured with primary cortical neurons or neuronal stem cells damaged by …
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Neuroprotection of AG490 in Oxidative Stress-induced Neuronal Death
… stress-induced cytotoxicity in cultured primary cortical neurons. AG490 significantly protected neuronal cell death against various oxidative stimuli, such as hydrogen peroxide (H2O2), L-buthionin-S,R-sulfoxamine (BSO), N-methyl-D-aspartic acid (NMDA), and arachidonic acid (AA). …
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Targeted Gene Therapy Approaches for Huntington's Disease: Advancing Treatment through Bioengineered Self-Assembling Peptide Hydrogels and BDNF-Encoded Adeno-Associated Viruses, Applied In Vitro and In Vivo
… protein. It mainly enhances the loss of striatal neurons and primary cortical neurons within the forebrain. Gene therapy has provided promising targeted therapeutic strategies to treat various neurodegenerative disorders such as HD, where the genomic DNA of a cell can be rewritten, reengineered, …
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Targeted Gene Therapy Approaches for Huntington's Disease: Advancing Treatment through Bioengineered Self-Assembling Peptide Hydrogels and BDNF-Encoded Adeno-Associated Viruses, Applied In Vitro and In Vivo
… protein. It mainly enhances the loss of striatal neurons and primary cortical neurons within the forebrain. Gene therapy has provided promising targeted therapeutic strategies to treat various neurodegenerative disorders such as HD, where the genomic DNA of a cell can be rewritten, reengineered, …
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GPR50, a potential factor involved in psychiatric disorders interacts with Alzheimer’s disease-related protein β- secretase (BACE1)
… in HEK293 cells, and at the neurites in mouse primary neuronal cells. Subcellular fractionation of adult mouse brain revealed that endogenous Gpr50 and Bace1 were co-fractionated in the presynaptic vesicles. Secondly, I showed that, in contrast to HEK293 cells, GPR50 overexpression had no …
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Analyzing A-series gangliosides in neurons following exposure to glutamate
Neurons within different brain regions have varying levels of vulnerability to external stress and therefore respond differently to injury. A potential reason to explain this may lie within a key lipid class of the cell’s plasma membrane called gangliosides. These glycosphingolipid species have …
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Neuronal Protection by a Novel C-terminal Hsp90 Modulator
… non-toxic Hsp90 modulator, KU-32, which protects primary cortical neurons against Aβ1-42, a toxic peptide found in AD brain. Pharmacokinetic studies indicated that KU-32 is blood brain barrier permeable and orally bioavailable. Using two AD mouse models, JNPL3 and rTg4510, which both contain the …
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Regulation of mGluR7 trafficking by SUMOylatio in neurons
… of full-length mGluR7 in mammalian cells and neurons. Here we have explored whether mGluR7 is a target of SUMOylation. Using biochemical approaches coupled with confocal imaging, we find that mGluR7 at Lys889, the sole SUMOylation site on the C-terminus of mGluR7 is a target of SUMO …
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Interplay of Brain Derived Neurotrophic Factor and Protein Kinase A in Modulating Mitochondrial Structure and Function in Brain Homeostasis and Disease
Abstract:Neurons are postmitotic cells that contain elongated specialized structures that emanate from their cell bodies termed neurites (axons and dendrites). In order to form neuronal networks, neurons rely on continuous neurotrophic support mediated by Brain-Derived Neurotrophic Factor (BDNF). …
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Investigation into the destructive and adaptive responses of neural cells to stress
… unit is essential to the longevity of neurons. Conversely, loss of homeostasis, particularly of Ca2+ levels, of redox balance and of ATP, contribute to neuronal loss and dysfunction in many neurodegenerative and neurological disorders. This thesis is centred on better understanding the …
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Examinations into the Calcium Hypothesis of Alzheimer's Disease
… level rescues exaggerated [Ca2+]i signaling in primary cortical neurons and hippocampal slices from mutant PS1 expressing mice. We then determined the contribution of exaggerated [Ca2+]i signaling to AD pathogenesis. Using a combination of genetic, biochemical, electrophysiological and …
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Utilizing Toxoplasma gondii To Understand Neuron-Specific Neuroinflammation
… persist for the life of the host by hiding in neurons in the Central Nervous System (CNS). It was thought that neurons permitted this chronic infection due to a lack of innate immune capabilities including decreased expression of cytokine and immune cell receptors, as well as an inability to …
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Neuroprotektive Effekte von Atorvastatin bei Glutamat-induzierter Exzitotoxizität in primären kortikalen Neuronen
Atorvastatin gehört zu einer Gruppe lipidsenkender Medikamente, die über eine kompetitive Hemmung des Schlüsselenzyms der Cholesterin-Biosynthese, der -HMG-CoA-Reduktase (-Hydroxymethylglutaryl-Coenzym A-Reduktase) wirken. Sie sind daher zur Therapie der Hypercholesterinämie (Stein et al. 2002) …
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Promoting Central Nervous System Regeneration by Targeting Intracellular Pathways
Adult central nervous system neurons regenerate poorly after injury. One reason for this regenerative failure is that a developmental change occurs where essential growth-molecules become excluded from axons with maturation. In this thesis, two strategies were employed in order to improve axon …
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Isopeptide and Ester Bond Ubiquitination Regulate Degradation of the Human Dopamine Receptor 4
… cognition and circadian rhythms. The hD4R is the primary dopaminergic receptor for the atypical anti-psychotic drug clozapine, which is used to treat schizophrenia and other cognitive disorders. The hD4R gene is unique among the superfamily of GPCR-encoding genes because within the human …
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Structural Properties of α-Synuclein in Functional and Pathological Contexts
… known as Lewy bodies that form inside the neurons of patients suffering from these conditions. A number of missense mutations, as well as duplications and triplications of the gene encoding αS have also been associated with familial forms of early onset PD. Despite the association between …