Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 27 for “"primary cilium"”.
-
Sensory Primary Cilium is a Distinct Signaling Compartment
<p>The primary cilium is a solitary cellular organelle that protrudes from the apical cell membrane. Findings on cilia-dependent mechanosenstation have shown that the primary cilium acts as a transducer of fluid-shear stress into intracellular signaling. Over recent years, studies in primary cilia …
-
Structural and biophysical characterization of membrane-coating proteins from the nuclear pore and the primary cilium
… pathway. Additionally, transport to the primary cilium is not well characterized and is therefore of interest. The nuclear envelope comprises a double membrane that fuses at points to produce pores, which link the nucleoplasm with the cytoplasm. Coating these openings are nuclear pore …
-
Cardiac repair post-myocardial infarction: Roles of the primary cilium and the long non-coding RNA Malat1
… factor Wilms’ tumor 1 (Wt1) and the primary cilium regulate EMT. Furthermore, the long non-coding RNA (lncRNA) Malat1 is not only known to regulate EMT, but also mitigates cell death from injury. Little is known about the roles of Wt1, primary cilia, and Malat1 in relation to neonatal …
-
Daple Orients and Patterns Sensory Hair Bundles in the Inner Ear
… for hair-bundle development. Although the primary cilium also participates in each of these pathways, the mechanism that links tissue-wide and cell-intrinsic polarity is not yet understood. To address this issue, I initiated a yeast two-hybrid screen with a central component of the …
-
Regulation of Inverted Formin-1 (INF1) by Microtubule-Affinity Regulating Kinase 2 (MARK2)
… show that INF1 overexpression is associated with primary cilium absence and in some cases, the presence of long cilia, suggesting that INF1 plays a role in primary cilium formation.
-
Stiffness controls primary cilia position and length: mechanisms and potential in reversing osteoarthritis phenotypes
… mechanosensitivity through the action of, the primary cilium. Mechanical stress and the stiffness of the extracellular matrix are key drivers of tissue development and homeostasis. Aberrant mechanosensation is associated with a wide range of pathologies, including diseases such as …
-
Chemically Based Interrogation of Smoothened, a Proto-Oncogenic Component of the Hedgehog Signal Transduction Pathway
… the cellular antenna-like structure known as the primary cilium. Furthermore, this deviant form of signaling from an intracellular compartment shelters SmoM2 from chemical attack thus rendering it intrinsically resistant to certain Smo antagonists. Based on this new understanding of SmoM2 …
-
A functional and therapeutic investigation of ciliopathy proteins and ciliopathies
… of genetic diseases whose aetiology lies in the primary cilium. Over 30 genes have been identified as mutant in ciliopathies and their proteins localise at the primary cilium. When mutated they can cause kidney disease, obesity, polydactyly, and retinal degeneration. In this project, I have …
-
The role of CD133 in mouse incisor tooth epithelium
… epithelium in CD133 KO mice revealed disturbed primary cilia homeostasis. Primary cilia are crucial in interpreting extracellular cues and CD133 KO primary cilia specifically fail to transmit Shh-mediated signals. Further investigation of the functions of CD133 revealed that CD133 is important …
-
Understanding HNF-1β through Identification of Interacting Proteins and Target Genes in the Kidney
… staining showed that PDE4C is localized in the primary cilium and there interacts with a protein complex containing AKAP150, adenylyl cyclase 5/6 (AC5/6), protein kinase A (PKA), and polycystin-2 (PKD2). These results identify a cAMP-regulating protein complex that is localized in the primary …
-
Targeting Primary Cilia Immune Receptor Proteins for the Treatment of Polycystic Kidney Disease Mechanisms
<p><strong>Background: </strong>Primary cilia are cellular organelles project from the cell surface of mammalian cell and play important roles in vertebrate development, organogenesis, health, and others genetic diseases. Primary cilium functions as a mechano-sensor and chemo-sensor. Defect in …
-
Establishment of hedgehog pathway overexpression in osteosarcoma cell lines leading to an anti-apoptotic genotype which can be inhibited by the common anti-fungal and hedgehog pathway inhibitor, itraconazole
Osteosarcoma (OS) is the most common primary bone cancer in dogs, yet therapies for pets with metastatic OS remain clinically ineffective. With the advent of precision medicine, strong scientific and clinical impetus exists to further investigate druggable molecular perturbations that might …
-
Functional Genomics Based Interrogation of Cell-Fate Determination Pathways
… Stk11 loss resulted in disassembly of the primary cilium, a cellular organizing center for Hh pathway components, thus dampening Hh signaling. Loss of Stk11 also induced aberrant signaling through the Wnt pathway. Chemicals that targeted the Wnt acyltransferase Porcupine or that restored …
-
A Novel Collapsing Response Mediator Protein 2 Binding Domain for Kinesin Light Chain 3
Significant similarity exists between primary cilia and sperm flagella. Both contain an axoneme and both require presence of ODF2. Therefore, primary cilia can be used as a partial model to study the assembly of sperm flagella. Recently, we have shown that Collapsin Response Mediator Protein 2 is …
-
Identification of a Novel ERK 1/2-Interacting A-Kinase Anchoring Protein
… in cells that are thought to contain a single primary cilium but not motile cilia. Immunofluorescence staining of primary cilia-containing cells indicates that RSP3/RSP3H localize to nuclear punctae, specifically promyelocytic leukemia (PML) bodies, suggesting a non-cilia related role for …
-
Morphogenesis of follicular epithelium in Drosophila melanogaster: function of von Hippel-Lindau tumour suppressor gene
… including maintenance and organization of the primary cilium, maintenance of the differentiated phenotype in renal cells and regulation of epithelial-mesenchymal transition. However, the function of h-VHL gene during development has not been fully understood. It was previously shown that d-VHL …
-
Structural characterization of primary cilia using accelerated piezoelectrically driven STED nanoscopy
Primary cilia are non-motile, hair-like projections occurring on most mammalian cell types. They play essential roles in transduction of chemical and mechanical signals across the cell membrane. For example, primary cilia are able to transduce sonic hedgehog signals, necessary in embryonic …
-
MOLECULAR CHARACTERIZATION OF THE PUTATIVE NUCLEOTIDE- BINDING AND HYDROLYZING ACTIVITIES OF THE ELLIS VAN CREVELD (EVC) PROTEIN
… together, they are tethered to the base of the primary cilium of cells. This multi-protein assembly positively regulates the Sonic and Indian hedgehog signaling pathways by promoting downstream processes after activation of the smoothened receptor (Smo), by the hedgehog proteins. Bioinformatic …
-
An in vivo study of novel genetic modifiers in autosomal recessive polycystic kidney disease (ARPKD)
… protein products of both genes localising to the primary cilium. Ciliary signalling pathways, such as Wnt signalling, have been investigated to dissect potential molecular mechanisms of ARPKD, however, these remain unclear. To overcome this, researchers have tried to develop rodent models that …
-
Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling
The primary cilium is a signaling organelle found on most cell types in vertebrates. It is a distinct compartment for membrane and cytoplasmic proteins that mediate multiple sensory processes. Little is known about the cellular and molecular mechanisms that determine regulated ciliary localization …
Page 1 of 2