Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 93 for “"primary cells"”.
-
Nuclease-Mediated Targeted Gene Insertion at the Adenosine Deaminase Locus in Primary Cells
… and the technique was extended to other forms of primary immunodeficiency. Unfortunately, some of the patients enrolled in these trials using retroviral vectors to carry replacement genes resulted in insertional oncogenesis. To avoid the insertional oncogenesis caused by random integration into …
-
Characterization of a new miniaturized hollow-fiber bioreactor for cultivation of cell lines and primary cells to improve cytostatic drug testing in vitro
… membranes. Due to a transparent housing, cells can be observed by microscopy during culture. The leukemic cell lines CCRF-CEM, HL-60 and REH were cultivated up to densities of 3.5 x 107/ml without medium change or manipulation of the cells. Growth and viability of the cells in the …
-
Effects of hypercapnic acidosis on the primary cells relevant to acute respiratory distress syndrome pathophysiology and the therapeutic potential of Mesenchymal Stem Cells
… on the inflammatory and reparative responses of primary human pulmonary microvascular endothelial cells (HPMECs) and primary human small airway epithelial cells (SAECs) – two of the primary cell types most relevant to the pathophysiology of ARDS – in an in vitro model of the condition. …
-
Prostate cancer stem cell fate: β-catenin and the Wnt pathway
… of a small number of prostate cancer stem cells within a tumour. Tumour initiation, maintenance and spreading have been attributed to this subpopulation of tumour cells. Wnt activity and β-catenin signalling have been associated with different types of cancer and microarray data from our …
-
Die Bedeutung von Zinkionen für mononucleäre Leukocyten
… important influences of zinc on mononuclear cells were examined.For the first time, a direct stimulation of cells by zinc could be shown, which was dependent on zinc concentration and could not be induced by other cations. Even in physiological doses, zinc interacts significantly with …
-
The electrochemical behaviour of zinc-cadmium based anode materials in potassium hydroxide
… the historical background to the development of primary cells, their performance characteristics and their fundamental electrochemistry.
-
Cell-type-specific responses to DNA damage from acute and chronic radiation
… of response variability between different cells, tissues and individuals. This thesis investigates normal tissue responses to DNA damage from acute and chronic radiation, including exploration of cell-type and individual-specific differences in DNA-damage response. Due to the difficulty of …
-
Effects of vitamin D on inflammation and oxidative stress in airway epithelial cells
… D may be beneficial in COPD. Airway epithelial cells play a central role in COPD pathogenesis and have been shown to constitutively activate vitamin D. This study investigated the effects of vitamin D on inflammation and oxidative stress in human airway epithelial cells, crucially on mediators …
-
Illuminating epithelial-stromal communication using engineered synthetic matrix microenvironments
… models, adaptable to the limited numbers of cells available from patient biopsies. In this thesis, I address a critical gap in accessible tools to study and control endometrial biology in vitro and do so in a manner that can be translated to other epithelial-stromal mucosal tissues. Using the …
-
The Safety and Immunostimulatory Properties of Amorphous Silica Nanoparticles <10 nm in Diameter
… using transformed and cancerous cell lines and primary cells. The particles were toxic to the immune cells in a dose dependent manner and impaired certain cellular functions. Primary cells were most susceptible to nanosilica induced death and, of the primary cells, phagocytes were most …
-
Imaging Chloride Homeostasis in Neurons
… epileptic-like discharges, and in glioblastoma primary cells, measuring osmotic swelling responses to various drugs treatment.
-
Gene Targeting in a Novel Mouse Model and the Chicken DT40 Cell Line
… genes, followed by transplanting the corrected cells back into the patient. However, the natural rate of gene targeting is too low to be of practical use in most cells; an exception to this is the chicken DT40 cell line which has a high relative rate of gene targeting (gene targeting rate/ …
-
Distinct Patterns of Ccr5 Versus Alternative Coreceptor Dependence in Non-Natural Host Versus Natural Host Simmian Immunodeficiency Virus Infection
… outcomes, including infection of distinct target cells in vivo. Cell targeting is substantially determined at the level of viral entry. Prior work demonstrated that sooty mangabey infection occurs in the absence of functional coreceptor CCR5, implicating alternative SIV entry pathways in this …
-
The Transcriptional Profile of Microglia: From Brain to Dish
… transcriptional profiles of microglia, both from primary human cells and the in-vitro model systems used to study the cells at scale. In this thesis, I aim to build on previous small-scale studies of primary microglia and in-vitro model systems to answer three major questions: 1. Can …
-
The Extent and Nature Of Chromosomal Rearrangements in B Lymphocytes
… rearrangements to a fixed DSB genome-wide, in primary cells. We examined over 180,000 rearrangements obtained from 400 million activated B lymphocytes to two loci, IgH and c-myc. Our data and analysis reveal that proximity between DSBs, transcriptional activity and chromosome territories are …
-
Microfluidics for Genetic and Epigenetic Analysis
… driven by the needs for testing scarce primary cells samples from patients in the context of precision medicine.
-
Control of Cardiac Hypertrophic Signaling by Inositol 1,4,5-Trisphosphate Receptors
… cell models can be used as an alternative to primary cells to study cardiac hypertrophy. Additionally, we show that all three IP3R proteins were expressed throughout the cardiomyocyte sarcoplasmic reticulum in neonatal and adult rat ventricular cardiomyocytes and in the human heart tissue. …
-
Development of microfluidic devices to maintain and interrogate tissue biopsies - biomedical applications
… disaggregated in situ on-chip into individual primary cells, using a collagenase digestion procedure, enabling further cell analysis to be carried out off-line. Cancer tissue was probed with chemotherapeutic drugs for the analysis of apoptotic biomarkers such as cytochrome c, nucleosomes and …
-
Preclinical evaluation of a novel telomerase inhibitor in malignant cells of the hematopoietic system
… with unlimited proliferation in most cancer cells. Recently, a non-nucleosidic small molecule inhibitor, BIBR1532, has been identified which is highly selective for the inhibition of telomerase resulting in telomere dysfunction and delayed growth arrest in tumor cells. Here we examined the …
-
Targeting MERTK on tumour cells and macrophages: a potential intervention for sporadic and NF2-related meningioma and schwannoma tumours
… and meningiomas, increasing tumour burden. The primary treatment for large or symptomatic schwannomas and meningiomas is surgical resection, which may not be suitable in cases involving tumours located near sensitive neuroanatomy or clinically vulnerable individuals. Not all schwannomas and …
Page 1 of 5