Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"primary cell culture"”.
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Developing cultivated mollusks through establishing primary cell culture methods of Eastern Oyster, Crassostrea virginica, as a model bivalve
… Cultivated seafood is made by growing animal cells in vitro using stem cells for edible food, eliminating the need to raise the entire animal. A crucial first step in developing cultivated seafood is creating a well-characterized cell line that can continuously grow and differentiate into …
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A NOVEL MAGNETIC THREE-DIMENSIONAL LEVITATED PRIMARY CELL CULTURE SYSTEM FOR THE DEVELOPMENT OF IN VITRO SALIVARY GLAND-LIKE ORGANOIDS
… to repair hypo-functional SG via various cell-based approaches in three-dimensional (3D) scaffold-based systems. The majority have nonhuman-based components. Therefore, this project aims to develop SG mini-glands/organoids using a novel 3D xeno-free system (M3DL) that assembles and …
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Development and characterization of an in vitro culture system as a physiological model for chronic Hepatitis B infection
… cases of chronic hepatitis, cirrhosis, and hepatocellular carcinoma. Significant disadvantages exist among currently available therapeutics (e.g. IFNca, lamivudine, adefovir, etc.) that include limited efficacy and the promotion of drug-resistant viral strains. These therapeutics are the research …
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The Role of Perivascular Fibrosis In Post-Stroke Glymphatic Impairment and Cerebral Amyloid Angiopathy
… I hypothesize that an abnormal pattern of extracellular matrix deposition within the vascular basement membrane, termed fibrosis, impairs Aβ drainage from the aged brain after stroke. I further hypothesize that inhibition of astrocytic transforming growth factor-β (TGF-β) signaling can reverse …
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Characterization of Dendritic Cells in the Bovine Mammary Gland
… pathogens could potentially target dendritic cells (DC). While there has been some research describing bovine DC populations in-vitro, little is known about DC in mammary tissue. In this study, immunohistofluorescence was used to identify and localize bovine mammary DC. DC were found in …
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Assessing fetal topoisomerase IIα as a mechanism of in utero benzene toxicity in generating double-stranded DNA damage in CD-1 mice
… stranded DNA (dsDNA) breaks in hematopoietic cells. My research examines the role of topo IIα as a target of benzene toxicity to generate dsDNA breaks using complementary in vitro and in vivo mouse models. We hypothesize that benzene exposure in utero will increase dsDNA breaks by perturbing …
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Characterization of the Epididymal Region of the Rooster
… Specific objectives were: (1) to develop a primary cell culture system of the efferent ductule epithelium; (2) to investigate the incidence and impact of epididymal stones on reproduction; and (3) to identify the ion transporters present in the rooster epididymal region, with an emphasis on …
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Biomarkers of Optic Nerve Head Glial Cell Activation Following Biomechanical Insult
… cause of irreversible blindness worldwide. Primary Open Angle Glaucoma is the most common form of the disease and can be characterized by the slow and irreversible apoptotic death of retinal ganglion cells, a unique optic nerve neuropathy resulting in loss of vision. Increased intra-ocular …
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Infectivity and Epidemiological Studies on Bovine Parvovirus
… signs of respiratory disease. Four established cell lines (bovine turbinates (BT), Madin Darby bovine kidney cells, Crandell feline kidney cells and porcine kidney15 cells), and two primary cell culture system (bovine embryonic kidney cells (BEK) and porcine primary kidney cells) were tested for …
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UNRAVELLING MECHANISMSOF SCHWANN CELLS IN VESTIBULAR SCHWANNOMA AND THEIR IMPACT ON HEARING LOSS.
… peripheral nerve tumour arising from Schwann cells (SCs) of the vestibulocochlear nerve (VN) and represents the most common tumour of the cerebellopontine angle. Although the loss of NF2/merlin is a well-established driver of schwannoma (SCH) formation, likely of the VS, the early biological …
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Pengaruh ekstrak pegagan (Centella asiatica linn.) terhadap pertumbuhan kultur primer sel hepar baby hamster yang dipapar 7.12- dimetilbenz(α)antrasen
… ENGLISH: Cancer is the growth and development of cells that are abnormal and uncontrollable. Environmental factors have been identified the cause of the occurrence of cancer in hepar derived from ingredients that are carcinogenic polycyclic aromatic compounds, such as hydrocarbons (PAH) i.e. 7.12- …
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Engrailed genes are cell autonomously required for the survival of the mesencephalic dopaminergic neurons
… factors En-1 and En-2, briefly, after the cells become postmitotic onwards. In mutant mice deficient for both genes, the neurons are induced at the proper location, express TH, but at a time, when En expression sets in, these neurons rapidly undergo apoptosis, so that at E14 the whole …
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Heterogeneity in vascular smooth muscle cell gene expression and its association with clonal proliferation in models of vascular disease
Deregulation of vascular smooth muscle cell (VSMC) phenotypic switching is implicated in vascular disease development and VSMCs produce the majority of cells in late-stage atherosclerotic plaques. However, VSMC accumulation in mouse models of vascular disease arises from clonal expansion of very …
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Elucidating the reversibility of ataxia
… motor deficiencies as well as reduced Purkinje cell firing frequency followed by dendritic tree degeneration and cell death. The aims of this study were to evaluate progression of Purkinje cell degeneration following loss of b-III spectrin function and determine whether the reintroduction of …
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I MICRORNA NELLE DISTROFIE MIOTONICHE
… in DM1 and DM2 patients on muscle biopsies and primary cell culture line. Using fast real time PCR, we report here the differences in miRNAs expression profiles between DM1 (n=15), DM2 (n=9) and control subjects (n=14) of 24 specific miRNAs. miRNAs expression profiles in muscle biopsies of DM1 …