Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"primary Hepatocytes"”.
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Transcriptional profiling of rat primary hepatocytes cultured in a 3D microfabricated liver reactor
Thesis (M.Eng.)--Massachusetts Institute of Technology, Biological Engineering Division, 2003.
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The Effect of Primary Hepatocyte Expressing HBx on the Activation and Apoptosis of Antigen-specific CD8+ T Cell
… apoptosis of CD8^(+) T cells co-cultured with hepatocytes expressing HBx were analyzed. First, recombinant baculovirus containing mammalian gene expression cassette for X gene was generated. Then, female MataHari naive CD8^(+) T cells were cultured with C57BL/6 mouse primary hepatocytes …
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Porcine model of xenobiotic metabolism
… hepatocyte cell line representative of human primary hepatocytes to support drug toxicity and metabolism assessments. Three independent hepatocyte cell lines were developed from three different Oncopigs and all of them expressed hepatocyte specific and most important drug metabolism and …
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The role of salt-inducible kinase in the control of hepatic gluconeogenesis
… not following insulin treatment or refeeding in primary hepatocytes and liver in vivo, respectively. Unexpectedly, fasting- or glucagon-stimulated phosphorylation of SIK2 (individually or in combination) did not directly modulate kinase activity of SIK2 and its subcellular localisation. These …
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Systems Engineering of Stem Cell Fate
… to maturation is still present when compared to primary hepatocytes. Comparison of transcriptome data of differentiation to embryonic liver development can elucidate the genetic roadblocks preventing ES cells from reaching the functional maturity of their tissue counterparts. Transcriptome data …
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Role of IL-6/gp130-dependent signalling pathways in hepatocytes during liver regeneration and inflammation
… of the Interleukin-6 (IL-6) receptor gp130 in hepatocytes was investigated in terms of liver regeneration, liver dependent iron homeostasis and gram positive sepsis. Therefore, mice either defective in hepatocyte-specific gp130-dependent Ras/Erk or STAT activation were generated to define the …
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Tailored peptide hydrogel-based three-dimensional system improves stem cell maintenance, therapeutic potential and differentiation
… treatments and differentiation. Liver hepatocytes, as an example, is in large demand not only for disease treatment but also for pharmaceutical development. However, hepatocytes derived from iPSCs are still functionally far from primary hepatocytes, there is a thus need to improve the …
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Engineered microtissue platforms for modeling human pathophysiology and drug metabolism
… liver injury. Phenotypic maintenance of primary hepatocytes was achieved by controlled pre-aggregation (-50 tm units) with J2-3T3 fibroblasts to establish cell-cell contacts prior to encapsulation into microtissues. Retention of both constitutive and inducible Phase I drug metabolism …
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Novel Insights Into Normal Aryl Hydrocarbon Receptor Biology Through the Regulation of Stanniocalcin 2
… test this hypothesis the current studies utilize primary hepatocytes to identify survival mechanisms consistent with normal AhR biology. Taking advantage of the Cre-lox system to manipulate AhR status, I report here that primary hepatocyte apoptosis is preferentially suppressed in cells expressing …
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Characterisation of Anti-Apoptotic Signalling Pathways in Hepatocytes activated by alpha-Lipoic Acid and Atrial Natriuretic Peptide
… of apoptotic cell death in two different models: primary hepatocytes and ischemic isolated perfused rat livers. alpha-lipoic acid was shown to protect isolated hepatocytes from TNF-alpha-/ActinomycinD-induced apoptosis. Astonishingly, this effect did not seem to be governed neither by its well …
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Genomic profiling and perturbation of Hepatitis B virus infection
… directed differentiation protocols to develop primary adult human hepatocyte and iPS-derived hepatocyte models of HBV infection, respectively, which enable us to test antiviral therapies with multiple mechanisms of action and identify a significant interferon-driven response to HBV infection. …
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A Systems Approach to Studying the Differentiation of Stem Cells Towards Hepatocytes
… to become any specific cell type, such as hepatocytes. A robust and efficient process for expansion and differentiation to generate large quantities of functional hepatocytes from stem cells will be essential to establishing a stem cell bioprocess in the future for therapeutic and …
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Novel Cancer Therapeutics, the Generation of ROS, and Cell Survival
… survival of renal cell carcinoma lines (RCC), primary renal epithelial cells, glioblastoma multiforme lines (GBM), and primary rodent astrocytes is unknown. The present studies examine whether the GST fusion protein, GST-MDA-7, and the adenovirus, Ad.mda-7, altered the growth and survival of …
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The Role of Interferon Stimlated Genes in Resistance and Immunity to Hepatitis C Virus Infection
… A functional genomics analysis of IFN treated primary hepatocytes resulted in the identification of eighty-six ISGs differentially induced by consensus interferon. Future evaluations of the role of these genes in HCV outcome, using integrated approaches as described here, will be invaluable in …
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Correlation of Heparan Sulfate Proteoglycan Structure and Metabolism With the Regulation of Cell Division
… of HS with cell growth was also observed in primary rat hepatocytes which do not divide in culture, in two rat hepatoma cell lines which exhibited no density-dependent inhibition of growth, and in cytolytic thymic lymphocytes which require interleukin 2 to proliferate. Primary hepatocytes …
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ROLE OF APOLIPOPROTEIN A-V IN TRIGLYCERIDE METABOLISM
… when endogenous mouse apoA-V was analyzed in primary hepatocytes. In addition to its low secretion efficiency, the electrophoretic banding pattern of apoA-V suggested that the protein is susceptible to intracellular proteolysis with ~60% undergoing presecretory turnover within 2 hours …
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Development of a Cytochrome P450 Expression System with Biomass Scale-up, Streamlined Downstream Processing and Stability Modifications
… activity, availability and consistency than primary hepatocytes. In this thesis, a recombinant P450 expression system in yeast was developed with the aim of providing a cost-effective way of producing human P450 enzymes upon the yeast’s endoplasmic reticulum (ER). These ER-bound P450s are …
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Hepatoprotective properties of Gentiana SPP: Against non-alcoholic fatty liver disease (NAFLD)
… requires further studies using unmodified primary hepatocytes to gain better understanding.
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Evaluation of the mechanisms of neurotoxcity using metabolomic strategies
… of mephedrone at 100 μM was first studied in primary rat hepatocytes and then the neurotoxicity of mephedrone at 100 μM was assessed in human neuroblastoma and astrocytoma cells using proliferation assays coupled with LC-MS based metabolomics.The …
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Cellular and Molecular Mechanisms Underlying Leptin's Metabolic Effects
… leak), and non-mitochondrial respiration of primary hepatocytes. We show that this is due to changes in the substrate oxidation system in liver mitochondria as well as changes in mitochondrial structure, and volume density. These changes are directly caused by leptin, and are not downstream …
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