Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"preinitiation complex"”.
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Preinitiation Complex Assembly and General Transcription Factor IIA
… competition assay. Using this assay, a partial preinitiation complex containing TFIIB is implicated as a target of GaI4-IE. These experiments, as well as others cited, suggest that activators affect early stages of PIC assembly, namely the formation of complexes including TFIID, TFIIA, and …
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Identifying a role for nonessential elF3 subunits eif-3.K and eif-3.L in the regulation of endoplasmic reticulum homeostasis and longevity in Caenorhabditis elegans
… initiation factor 3 (elF3) is a protein complex composed of 13 subunits in mammals, and is an essential scaffold of the molecular interactions required for the formation of the 43S preinitiation complex (PIC). While these 13 subunits are broadly conserved within the eukaryotic phylogeny, …
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The Role of the ORB2 RNA-binding Protein in Post-transcriptional Regulation
… includes protein components of the 43S preinitiation complex of translation initiation and components of the Cup repressive complex. I found that the ZZ domain is necessary for ORB2’s association with both complexes. Finally, I show that deletion of the ZZ domain of ORB2 causes major …
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The Role of Phosphorylation in Modulating PC4 Function as a General Cofactor
Purification of multisubunit protein complexes such as human TFIIH and RNA polymerase II has been a tedious task by conventional chromatography. To overcome the difficulty in isolating human TFIIH and RNA polymerase II, which play an essential role in eukaryotic transcription, we have developed an …
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Eukaryotic Transcriptional Activation Mechanism: Protein-Protein Interactions
… activators target TFIID (TBP)-TFIIB promoter complex formation in the preinitiation complex assembly process by inducing (or stabilizing) qualitative or quantitative alterations within TFIID (TBP)-TFIIB-promoter complexes that consequently enhance recruitment of downstream initiation factors. …
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Studying sequence effects of mRNA 5' cap juxtapositions on translation initiation rate using randomization strategy of the extreme 5' end of mRNA
Translation initiation is a complex process. The efficiency of translation initiation is determined not just by activity and availability of the translation initiation apparatus, but also the properties of mRNA 5’transcript leaders (5’TL). In most cases of cap-dependent translation, translation …
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Purification and characterization of TFIIIA from Acanthamoeba castellanii
… that is assembled onto the DNA to form the preinitiation complex. Previous work in our laboratory has shown that A. castellanii TFIIIA is under direct regulatory control. During the process of A. castellanii encystment, both ribosomal RNA (rRNA) and 5S RNA transcription are coordinated …
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Transcription regulation in Plasmodium falciparum : functional characterisation of general transcription factor IIB
… by distinct morphological changes. The complex lifecycle, along with the ability to adjust rapidly to different environmental niches, is governed by highly regulated and tightly synchronised changes in stage-specific gene expression. In eukaryotes, regulation of RNA Polymerase II …