Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"pre-mRNA splicing"”.
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Algorithms for simulating human pre-mRNA splicing decisions
… ExonScan, to simulate constitutive human pre-mRNA splicing. ExonScan includes several models for splicing components, including splice sites, exonic splicing enhancers, exonic splicing silencers, and intronic splicing enhancers. I used ExonScan to test various aspects of human splicing, …
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The role of U1 and snRNP in pre-mRNA splicing
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.
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Studies on mammalian pre-mRNA splicing : connections to transcription and cancer
This thesis presents two separate pieces of work pertaining to pre-mRNA splicing in mammalian cells. The first part examines the regulation of the alternative splicing of the PKM gene in cancer cells, while the second part investigates the physical connections between the transcriptional apparatus …
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In Vivo Analysis of Pre-Mrna Splicing in Dicot Plant Nuclei
In vivo gene transfer experiments have suggested that the elements mediating intron recognition differ in monocot and dicot nuclei.
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Interpretable Computational Modeling of pre-mRNA Splicing for Multiple Eukaryotic Species
One of the key steps in eukaryotic gene expression is pre-mRNA splicing, whereby intronic sequences are excised from immature pre-mRNA transcripts and the remaining exonic sequences are joined together. This process is catalyzed by the spliceosome, a large complex of proteins and RNAs. A variety of …
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Role of SMU Homologues in Pre-mRNA Splicing During Maize and Arabidopsis Development
… that it contained a genesimilar to the smu-2 (suppressor of mec-8 unc-52) gene in nematodes. Previous studies showed that the mutations in either thenematode smu-1 or smu-2 genes affect splicing of the unc-52 pre-mRNA, and SMU-1 protein interacts with SMU-2 protein. In addition, human homologues …
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Regulated T cell pre-mRNA splicing as genetic marker of T cell suppression
Includes abstract. Includes bibliographical references.
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Differential Impact of VEGF and FGF2 Signaling Mechanisms on Flt1 Pre-mRNA Splicing
… from a limited number of genes via alternative splicing, where one gene gives rise to multiple proteins. Alternatively spliced gene products, although crucial for normal physiology, are also linked to an increasing number of pathologies. Consequently, a growing focus is currently being placed on …
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The Role and Fate of Branch Site-U2 snRNA Interaction During Pre-mRNA Splicing
… dynamics underlying transitions during premRNA splicing, particularly during the catalytic phase, remain unclear despite our deep knowledge of spliceosome composition. The complex assembly phase that precedes catalysis, and the involvement of several motifs in multiple interactions along …
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Essential Protein Factors in pre-mRNA splicing : A Structural Study by Nuclear Magnetic Resonance Spectroscopy
… that play important roles in eukaryotic RNA splicing. These results are discussed in Chapters 1 and 2, while biomolecular NMR techniques that were employed for the structure determination are outlined in Chapter 3. Materials and methods are described in Chapter 4. Chapter 1 presents the …
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Molecular analysis of wheat snRNAs
Pre-mRNA splicing is a complex event in which introns are removed and exons spliced together. This process is mediated by small ribonucleoprotein particles (snRNPs), formed from small nuclear RNAs (snRNAs) and their associated nuclear proteins.
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Su onkologinėmis ligomis siejamas pre-iRNR splaisingas: splaisingo veiksnių ir hipoksinės mikroaplinkos tyrimas /
… mortality has been systematically increasing. As pre-mRNA splicing is a highly important process in regulating gene expression, it is not surprising that changes in splicing have been observed in many different diseases. Unfortunately, the reasons for the expression of disease-associated mRNA …
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Sequences and factors required for 5' splice site selection in Schizosaccharomyces pombe
… our knowledge of how this sequence functions in pre-catalytic events in pre-mRNA splicing. To determine the contribution to U1 function of each position complementary to the first six nucleotides of the intron, I mutagenized the relevant U1 nucleotides (1 through 6) and examined the ability of …
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Validating the WD Repeat Protein CDC40 as a Potential Therapeutic Target for Lung Cancer
… most diagnosed and most lethal cancer globally. Presently, there is a need for novel targets for the development of improved therapeutics. Data mining of whole genome genetic dependency screens suggested that the pre-mRNA splicing factor Cell Division Cycle 40 (CDC40) is essential for lung cancer …
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Characterization of nonsense mediated mRNA decay in Schizosaccharomyces pombe
Nonsense mediated mRNA decay (NMD) is a translation-coupled process that preferentially destroys mRNAs harboring premature translation termination codons (PTCs). In mammalian cells, NMD is linked to pre-mRNA splicing. Typically, PTCs elicit strong NMD only if positioned upstream of at least one …
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Identification of novel branch points reveals insights into RNA processing
Pre-mRNA splicing is a ubiquitous process necessary for the production of functional eukaryotic mRNAs. The branch point (BP) sequence is one of three key nucleotide sequences required for pre-mRNA splicing, however, in metazoa it has been less comprehensively studied than the 5' splice site (5'SS) …
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The splicing inhibitor Isoginkgetin leads to defects in multiple phases of the cell cycle
… of spliceosome assembly. The p53-tumour suppressor plays a prominent role in ell G1 and G2 cycle checkpoints and p53 is strongly activated in response to transcriptional stresses. Therefore, we determined the effect of p53 and Isoginkgetin treatment on cell cycle progression. Wildtype p53 …
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Identifying modifier genes in SMA model mice
… complex which take part in snRNP biogenesis and pre-mRNA splicing. Without SMN protein, motor neurons die, and nerve impulses are not passed between the brain and muscles. As a result, some muscles cannot perform their normal functions, leading to weakness and impaired movement. In this research, …
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Discrete elements of the U2 snRNP regulate its intranuclear trafficking and recruitment to nascent transcripts
… snRNP association with nascent transcripts and splicing speckles, while the Sm binding site is sufficient for U2 snRNP association with Cajal bodies after nuclear entry. For SL4, I further show that the associated protein U2B” rather than SL4 itself provides the targeting activity, which …
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Novel RNA Targets of the Spinal Muscular Atrophy Protein
… translation and the spliceosome which catalyzes pre-mRNA splicing. The biogenesis of RNPs often involves complex and elaborate pathways involving post-translational modifications, transit into specific cellular domains, and several auxiliary factors including assembly chaperones. One of the …
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