Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 126 for “"pre-mRNA"”.
-
Algorithms for simulating human pre-mRNA splicing decisions
… ExonScan, to simulate constitutive human pre-mRNA splicing. ExonScan includes several models for splicing components, including splice sites, exonic splicing enhancers, exonic splicing silencers, and intronic splicing enhancers. I used ExonScan to test various aspects of human splicing, …
-
Nonsense-mediated mRNA reduction and pre-mRNA processing in drosophila
From bacteria to mammalian cells, the presence of a nonsense mutation causes a reduction in the level of the mRNA of the corresponding gene. The reduction is not, contrary to initial expectations, due to a passive mechanism by which non translated mRNAs are degraded; rather it is a active process …
-
The role of U1 and snRNP in pre-mRNA splicing
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.
-
Studies on mammalian pre-mRNA splicing : connections to transcription and cancer
This thesis presents two separate pieces of work pertaining to pre-mRNA splicing in mammalian cells. The first part examines the regulation of the alternative splicing of the PKM gene in cancer cells, while the second part investigates the physical connections between the transcriptional apparatus …
-
In Vivo Analysis of Pre-Mrna Splicing in Dicot Plant Nuclei
In vivo gene transfer experiments have suggested that the elements mediating intron recognition differ in monocot and dicot nuclei.
-
Interpretable Computational Modeling of pre-mRNA Splicing for Multiple Eukaryotic Species
One of the key steps in eukaryotic gene expression is pre-mRNA splicing, whereby intronic sequences are excised from immature pre-mRNA transcripts and the remaining exonic sequences are joined together. This process is catalyzed by the spliceosome, a large complex of proteins and RNAs. A variety of …
-
Integrative analysis of transcriptomic data to elucidate regulators of pre-mRNA processing
Alternative pre-mRNA processing events, including alternative splicing (AS) and alternative polyadenylation (APA), are key drivers of transcriptomic and proteomic diversity. This dissertation presents a comprehensive framework for analyzing these events, emphasizing improvements to computational …
-
Role of SMU Homologues in Pre-mRNA Splicing During Maize and Arabidopsis Development
… that it contained a genesimilar to the smu-2 (suppressor of mec-8 unc-52) gene in nematodes. Previous studies showed that the mutations in either thenematode smu-1 or smu-2 genes affect splicing of the unc-52 pre-mRNA, and SMU-1 protein interacts with SMU-2 protein. In addition, human homologues …
-
Regulated T cell pre-mRNA splicing as genetic marker of T cell suppression
Includes abstract. Includes bibliographical references.
-
Differential Impact of VEGF and FGF2 Signaling Mechanisms on Flt1 Pre-mRNA Splicing
… splice variants, sFlt1_v1 and sFlt1_v2, in the pregnancy-related disorder, preeclampsia. Preeclampsia is characterized by proteinuria and hypertension and is responsible for almost 600,000 maternal and fetal yearly deaths, worldwide. Here, we examined the impact of endothelial mitogens VEGF and …
-
The Role and Fate of Branch Site-U2 snRNA Interaction During Pre-mRNA Splicing
… dynamics underlying transitions during premRNA splicing, particularly during the catalytic phase, remain unclear despite our deep knowledge of spliceosome composition. The complex assembly phase that precedes catalysis, and the involvement of several motifs in multiple interactions along …
-
The Role of ATP and Cleavage Factor Phosphorylation in pre-mRNA 3' Cleavage Reaction
… in generating a functional messenger RNA (mRNA) transcript. It is long-known that ATP can significantly stimulate the in vitro cleavage of adenovirus type 2 L3 (Ad2L3) RNA substrate. Here, we used ATP analogs in structure-activity assays to show that the structural features of ATP and its …
-
Essential Protein Factors in pre-mRNA splicing : A Structural Study by Nuclear Magnetic Resonance Spectroscopy
… methods are described in Chapter 4. Chapter 1 presents the solution structure of the Tudor domain of the human Survival of Motor Neuron (SMN) protein and its molecular interaction with the spliceosomal Sm proteins. Sm proteins are common components of small nuclear ribonucleoprotein particles …
-
Structure determination of a yeast ribosomal protein L30 and pre-mRNA binding site complex by NMR spectroscopy
… ribosomal protein L30 and its auto-regulatory pre-mRNA binding site provide one of the best examples the critical role of protein-RNA interactions in regulation of RNA processing and control of gene translation. A model system for this interaction, which includes the ribosomal L30 protein and …
-
Recognition of the pyrimidine-tract of the pre-mRNA by U2AF and a novel splicing factor PUF60
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1998.
-
In vitro reconstitution of the human pre-mRNA 3′ end processing machinery and mechanistic insights into endonuclease activation
… entails endonucleolytic cleavage of the nascent pre-mRNA and addition of a poly(A) tail to the resultant free 3′ end. The poly(A) tail then facilitates nuclear export of mRNAs and controls their stability and translational efficiency in the cytoplasm. Pre-mRNA 3′ end processing is also tightly …
-
Identification of the RNA Cis-Elements that Interact with SRp30a to Regulate the Alternative Splicing of Caspase 9 Pre-mRNA
… important for binding SRp30a and required for expression of the caspase 9a splice variant. In vitro binding assays coupled with competitor studies demonstrated specific binding of RNA trans-acting proteins and SRp30a with these sequences. Furthermore, SDS-PAGE analysis of cross-linked RNA …
-
Tissue specific genetic regulation of Interleukin 6
… identified and reported to associate with IL6 expression. However, conflicting results have emerged and both the relationship between IL6 and vascular disease and the precise effect of SNP G-174C in vivo in humans remains obscure.<br/>The aim of this study was to establish the effect of SNP …
-
Spliceosomal small nuclear ribonucleoprotein particle trafficking and maturation in the nuclear compartment
… of functional snRNPs, are targeted to elongating pre-mRNAs. This finding presents us with a novel assay with which to dissect the mechanism by which snRNPs are targeted to nascent pre-mRNA transcripts. Two critical advantages offered by this system are immediately evident. First, it allows us to …
Page 1 of 7