Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"pre-B-cells"”.
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Towards a B-Lymphoid Model of E2A-PBX1-Mediated Leukemogenesis: Evaluating the Impact of Hematopoietic Cell of Origin on the Transformation Properties of a Leukemogenic Transcription Factor
The t(1;19) chromosomal translocation is present in 5% of acute lymphoblastic leukemia (ALL) cases and leads to expression of the oncogenic transcription factor, E2A-PBX1. Although t(1;19) is exclusively associated with pre-B ALL in clinical cases, murine models produce myeloid or T-lymphoid …
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Analysis of B lymphocyte activation and differentiation by expression profiling
… of SLP65 adaptor protein. SLP65-deficient B cells are arrested at the large cycling pre-B cell stage in the bone marrow and transitional B cell stages in the spleen of mice. The zinc-finger transcription factor EGR1 is an immediate early gene induced upon Ag-binding and BCR engagement in B …
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Role of AID and microRNA-155 in c-myc-IgH Translocations
… 2 (RAG1/2) during antigen receptor assembly in pre- B cells, suggesting that these translocations might be the result of aberrant V(D)J recombination. However, in mature B cells undergoing AID dependent somatic hypermutation (SHM), duplications or deletions that would necessitate a double strand …
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Identification and functional characterization of the novel MAR-binding protein, SATB2
The regulation of gene expression is governed in large part by transcription factors that bind to enhancers and promoters. The functions of transcription factors involve both the modulation of chromatin accessibility via the recruitment of histone-modifying enzymes or nucleosome-remodeling …
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The cloning and characterization of the TIK kinase.
… TIK kinase was isolated and cloned from murine pre-B cells on the basis of its immunoreactivity with antibodies to phosphotyrosine, and was therefore originally thought to be a protein tyrosine kinase. Characterization of the in vitro and in vivo activity of this kinase, however, revealed that …
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Mechanism for Pre-B Cell Loss in Mutant Ali/Ali Rabbits
<p>Pre-B cell receptor (pre-BCR) signaling is a critical checkpoint in B cell development in which B-lineage cells expressing functional μ-chains undergo selective expansion. My dissertation work used a rabbit model system to study how VH structure affects the early stages of B cell development in …
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Elucidating oncogenic mechanisms in human B cell malignancies
… Diffuse Large B Cell Lymphoma (DLBCL). Firstly, Pre-B ALL represents the most common paediatric malignancy and despite increasingly improved outcomes for patients, ~ 20% of all patients diagnosed with ALL relapse. Activating mutations in the RAS pathway are common (~50%) and result in …
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STAT5 regulierte differentielle Genexpression in prä-B-leukämischen Zellen
… Display auf Unterschiede in der Genexpression untersucht. Grundlage für diese Experimente war die Hypothese, daß eine Subtraktion des Genexpressionsmusters unstimulierter BaMR Zellen von der Genexpression der BaMR Zellen zur Identifizierung von STAT5 regulierter Gene führen würde. Es …
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Investigation of the three dimensional organisation of the genome in B cell development
… view of murine Igh locus conformation in pro-B cells, resolving conflicting models in the field. I observed that the Igh locus folds into subdomains and two regulatory elements that bind CTCF, the 3’ CTCF Binding Elements (3’CBEs) and the Intergenic Control Region 1 (IGCR1), mediate contacts …
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Spatial organisation of the immunoglobulin heavy chain locus and inter-chromosomal gene networks driving B cell development
… the variable region of the antibody. Gene expression depends on spatial organisation of chromatin. To ensure that all V genes have a chance to recombine, they are brought into physical proximity to the D-J region by locus contraction and DNA looping. Not all V genes recombine with equal …
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Discovery of miRNA-regulated processes in mammalian development
… targeted mRNA. Computational estimates based on prediction of conserved seed matches in 3'UTRs suggest that over half of genes in mammalian genomes are miRNA-regulated. Many miRNAs are also ubiquitously expressed in a variety of embryonic and adult tissues. Given these observations, we have …
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CATALYTIC-INDEPENDENT FUNCTIONS OF KDM6A IN B CELL MATURATION AND IMMUNE REGULATION
… involved in epigenetic regulation of gene expression. It catalyzes the demethylation of histone H3 lysine-27, counteracting the function of Polycomb Repressive Complex 2 (PRC2). Kdm6a plays a key role in transcriptional activation of genes that control cell differentiation during embryonic …