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Showing 1 to 20 of 21 for “"ppGpp"”.
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Development of in vivo ppGpp reporters in escherichia coli
ppGpp is a small molecule that works as a global master regulator of the E. coli physiology, most notably during the stringent response. Systems biology studies have emphasized its role in understanding the host response to the burden of introduced synthetic genetic circuits. Traditionally, …
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Estado viável não cultivável em Salmonella enterica sorovar Enteritidis deficiente na síntese de (p)ppGpp
… adversas. S. enterica é capaz de produzir (p)ppGpp, um nucleotídeo responsável pelo controle da expressão gênica no nível da transcrição e da tradução, em condições de estresse nutricional. Em razão da expressão gênica determinada pelo acúmulo de (p)ppGpp, S. enterica suporta condições …
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Relative contributions of the stringent response mediators (p)ppGpp and DksA to Haemophilus ducreyi virulence in humans
… by guanosine tetra- and pentaphosphate [(p)ppGpp] and the transcriptional regulator DksA. (p)ppGpp and DksA jointly interact with RNA polymerase to regulate genes critical for bacterial survival. We hypothesized that the stringent response is required for H. ducreyi virulence in humans. A …
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Guanosine 5'-diphosphate 3'-diphosphate (ppGpp), guanosine 5'-diphosphate 3'-monophosphate (ppGp) and antibiotic production in Streptomyces clavuligerus
… guanosine 5'-diphosphate 3'-diphosphate (ppGpp) were detected at D= 0.02 h' in continuous culture in all media. Low levels of intracellular guanosine 5 '-diphosphate 3 '-monophosphate (ppGp) were detected at D=0.02 if' in carbon- and nitrogen- limited media. ppop was not detected under …
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THE EFFECT OF STRINGENT CONTROL ON THE PRODUCTION OF ENTEROTOXIN, TRYPTOPHANASE, AND BETA-GALACTOSIDASE BY ESCHERICHIA COLI IN A RECYCLING FERMENTOR
… of guanosine 5'-diphosphate 3'-diphosphate (ppGpp) within the cell. By comparing the growth patterns of three enterotoxigenic Escherichia coli strains to that of E. coli strains of known rel genotypes, the Rel phenotypes of the enterotoxigenic strains were determined.</p><p>Both enterotoxin …
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REGULATORY PROCESSES IN ULTRA SLOW GROWING MICROORGANISMS (ESCHERICHIA COLI, BACILLUS POLYMYXA)
… of hours. Guanosine 5-diphosphate 3-diphosphate (ppGpp) accumulation commences at the beginning of phase 2 in E. coli, reaching a maximum at the start of phase 3, concurrent with the slowing of RNA synthesis. This final growth stage then is an indefinitely prolonged state of regulation by the …
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Structural and Functional Studies on the Escherichia coli Inducible Lysine Decarboxylase: Linking the Acid Stress and Stringent Responses
… with the stringent response alarmone ppGpp. The stringent response is activated under nutritional and stress conditions and reorganizes cellular transcription and metabolism from exponential-phase growth into stationary phase growth. The primary target of ppGpp is the RNA polymerase, …
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Transcription in Mycoplasma pneumoniae
… der Rolle von RelA und des Signalmoleküls (p)ppGpp in M. pneumoniae wurden durchgeführt. Während gezeigt werden konnte, dass die Mechanismen, die zur RelA-abhängigen Synthese von (p)ppGpp in diesem Organismus konserviert sind, konnte, abgesehen von der Unfähigkeit (p)ppGpp herzustellen kein …
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Characterization of Bacterial Transcriptional Regulatory Networks through Genome-Wide in vitro Run-Off Transcription RNA-Seq (ROSE)
… K-12 MG1655. The protein DksA and the alarmone ppGpp were added to extend the ROSE method, resulting in 2,205 TSS with differential transcription activity of at least twofold in the presence of DksA+ppGpp. Out of these, 1,387 TSS showed a positive regulation, and 818 TSS showed a negative …
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Characterization of beneficial mutations in unsaturated fatty acid biosynthesis that are recurrent dead-ends in a long-term evolution experiment with Escherichia coli
… SpoT to produce the cellular "alarmone" (p)ppGpp, inhibiting cell growth during the stringent response. Thus, it is possible that fabR mutations that prolong fatty acid synthesis and spoT mutations that alter the production of (p)ppGpp may both benefit cells by affecting the stringent …
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Integrated modeling of bacterial metabolism
… model of proteome partitioning centering on ppGpp-mediated regulation, we find that the ability of E. coli to optimize its proteome lies in an ultrasensitive, negative feedback controlling topology, with the ultrasensitivity arising from zero-order kinetics of tRNA aminoacylation and …
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Cryo-EM Studies of Bacterial RNA Polymerase
… response depends on two factors, the alarmone ppGpp, and the transcription factor DksA. Unlike most transcription factors, ppGpp and DksA bind directly to RNAP but not to DNA. Another transcription factor, TraR, regulates RNAP the same way as ppGpp and DksA. TraR, and ppGpp/DksA, can activate …
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The role of highly phosphorylated nucleotides in the development of bacterial resistance to antibiotics
… of highly phosphorylated purines (HPNs; MSI, ppGpp; MSII, pppGpp) produced by bacterial cells during the late exponential and early stationary phases of growth, in response to adverse nutritional conditions. These compounds are involved in the phenomena of endosporulation and stringent …
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The effects of nutrient limitation and growth rate on the regulation of the multi-drug pump genes acrAB in escherichia coli
… AcrR than the transcription of acrR. The role of ppGpp in the regulation of acrAB or acrR expression was not established in these studies, however, evidence was presented for positive regulation by cyclic AMP, and the possible role of negative supercoiling was discussed.
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Mathematical Model of the Cell Cycle Control and Asymmetry Development in Caulobacter crescentus
… and guanosine pentaphosphate or tetraphosphate (pppGpp or ppGpp), under normal and stressful conditions. This research suggests that the RelA-SpoT homolog enzymes have the potential to effectively influence the cell cycle in response to nutrition changes by regulating cdG and (p)ppGpp levels. We …
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Posttranscriptional and posttranslational regulation of virulence in erwinia amylovora
… hrpX expression was down-regulated in the ppGpp-deficient mutant and the dksA mutant, but up-regulated when the wild-type strain was treated with serine hydroxamate, suggesting that ppGpp might induce hrpX/hrpY and hrpS expression. Furthermore, CsrA positively regulates hrpS expression …
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Characterization of the Omega Subunit of Borrelia Burgdorferi’s RNA Polymerase
… ? subunit during gene transcription, and bind to ppGpp to react to environmental stress. Open reading frame bb0820 of the B. burgdorferi genome is annotated as rpoZ, however, its function has yet to be tested. The goal of this study was to determine the functional role of RpoZ in B. burgdorferi. …
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Studies on stationary phase Vibrio sp. 2
… markedly increases. The magic spot nucleotides, ppGpp and pppGpp, which are present in stressed exponential phase Vibrio sp. 2 cells, are not detectable in stationary phase Vibrio cells. The specific proteolytic activity of shaking stationary phase cell-free extracts against the foreign protein …
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Characterization of A transcriptional Attenuator in The rpmf-Plsx-Fab Operon of Escherichia coli K-12
… binding to attenuator mRNA, the PlsX protein, ppGpp concentration, and rate of lipid synthesis all appear to have no effect on attenuator activity. Interestingly, growth temperature appears to have an effect on both attenuator activity and the activity of one or more of the promoters upstream …
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Modeling and controlling resource loading in bacterial genetic circuits
… with sole hydrolysis activity, which lowers ppGpp level and thus de-represses ribosomes. That is, the controller increases the availability of resources once they are demanded by the GOI activation by actuating the cell’s endogenous ribosome regulation system. Without the controller, …
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