Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"potyviruses"”.
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Identification of two potyviruses of phaseolus vulgaris in South Africa
… (EM) indicated that these viruses could be potyviruses, as they were flexuous particles of approximately 700 to 800 nm. Observation of pinwheels in ultrathin sections of Nicotiana benthamiana infected with isolate 93/1 and Phaseolus vulgaris infected with isolate 93/65, confirmed that the …
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Plant Virus Diagnostics: Comparison of classical and membrane-based techniques for immunoassay and coat protein sequence characterization for Cucumber mosaic virus and three potyviruses
… Methods were developed with CMV and three potyviruses, using the same membranes, for detecting viral RNA by RT-PCR and direct sequencing of PCR products.. Amplification by RT-PCRÂ was possible after membrane storage for up to 15 months. The membranes also performed well with samples sent …
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Dynamics of the sweetpotato potyvirus aphid pathosystem in Louisiana
Sweetpotato potyviruses [Sweet potato feathery mottle virus (SPFMV), Sweet potato virus G (SPVG) and Sweet potato virus 2 (SPV2)] commonly infect sweetpotato and weedy morning glories in the USA. These viruses are transmitted in a non-persistent manner by various aphid species and cause up to 15% …
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Molecular characterization of viruses infecting potato and vegetables in Iraq
… (RT-PCR) were performed to screen samples for potyviruses, begomoviruses, carlaviruses, tombusviruses, potexviruses, cucumoviruses, tobamoviruses and alfamoviruses. Circular DNA viruses were screened by rolling circle amplification (RCA). Products resulting from PCR/RT-PCR and RCA were cloned …
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Construction and analysis of the pangenome of Cucurbita moschata to uncover variants associated with virus resistance
… crop that is severely affected by several potyviruses, including Moroccan watermelon mosaic virus (MWMV), zucchini yellow mosaic virus (ZYMV), and papaya ringspot virus (PRSV). The development of molecular markers linked to virus resistance can greatly assist breeding programs through …
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Site Directed Mutagenesis of a Putative Protease Cleavage Site Within the 105 Kilodation Protein of Southern Bean Mosaic Virus
… of known cleavage sites of picornaviruses and potyviruses; 2) computer prediction of the cleavage products from the SBMV RNA sequence; 3) observed in vitro translation products. In order to investigate the role of the C60 QS pair in the proteolytic processing of the 105 KDa polyprotein, a …
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Epidemiology, Aphid Vectors, Impact and Management of Tobacco Etch Potyvirus in Hot Peppers in Jamaica
… Jamaica is constrained by the aphid-transmitted potyviruses, tobacco etch virus (TEV) and potato virus Y (PVY). The virus epidemiology was not understood and no effective virus management system existed for these viruses. This study sought to identify possible management strategies for …
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High-Resolution Mapping of the Region around the Soybean Virus Resistance Genes, Rsv1 and Rpv1
… virus (SMV) and peanut mottle virus (PMV) are potyviruses that can cause serious yield reductions in soybean [Glycine max (L.) Merr.]. Virus resistant soybean cultivars have been released with alleles at the Rsv1 and Rpv1 locus that confer resistance to SMV and PMV, respectively. A …
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Engineering resistance to maize lethal necrosis
… in line with previous reports on SCMV and potyviruses. These data also produced first reports of a number of RNA viruses in East Africa, and five novel viral-like sequences, with their presence confirmed by RT-PCR. RNA silencing is an important component of the plant immune response to …
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Evolutionary studies of wheat streak mosaic-associated viruses and characterization of the wheat virome
… sharing significantly low (<40%) similarity with potyviruses and recently discovered virga-like viruses were identified. Our analysis showed that these viral sequences belong to a novel putative virus tentatively named Wheat-associated vipovirus (WaVPV). Additionally, fungal-associated viruses, …
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Soybean mosaic virus: strains, ultrastructure and movement
… to be similar to that reported for other potyviruses. The observation of pinwheels in the paraveineal mesophyll cells suggested the involvement of these cells in virus translocation within the soybean leaf. A sinusoidal pattern of SMV particle concentration within the inoculated and …
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Tamarillo mosaic potyvirus: characterization and resistance
… future mechanisms for obtaining resistance to potyviruses.