Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"potassium efflux"”.
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Development of a Biosensor to Predict Activated Sludge Deflocculation, and the Link Between Chlorination and Potassium Efflux
… that the source initiates. The Glutathione-gated potassium efflux (GGKE)induced activated sludge deflocculation biosensor incorporates novel microtechnology with a whole cell biological element to predict deflocculation from electrophilic sources. This sensor utilizes microfluidic channels to …
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Investigating the Impact of K+ Channel Expression on Mechanosurveillance of Disseminated Cancer Cells
… remain poorly understood. Here, we identified potassium efflux and KCNMB1, an auxiliary subunit of the large conductance potassium efflux (BK) channels, as regulators of cellular stiffness downstream of myocardin related transcription factor A (MRTFA). In primary pericytes, KCNMB1 knockdown …
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Evaluating strategies for integrating bacterial cells into a biosensor designed to detect electrophilic toxins
… selected in this work is the glutathione-gated potassium efflux (GGKE) system, which responds to electrophilic stress by effluxing potassium from the interior to the exterior of the cell. Thus, the bulk potassium in solution can be monitored as an indicator of bacterial stress. By utilizing this …
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Evaluating bacterial cell immobilization matrices for use in a biosensor
… proposed that contains bacteria that naturally effluxes potassium ions when threatened by electrophilic species. Pseudomonas aeruginosa is an activated sludge isolate and possesses the characteristic potassium efflux response. It has been immobilized in calcium alginate beads, photopolymer …
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Potassium and Sodium Movements Related to Growth State and Malignant Transformation in Cultured Chicken Embryo Cells
Aspects of sodium-potassium pump regulation were examined in growing normal, density-inhibited, and Rous sarcoma virus-transformed (RSV-transformed) chicken embryo fibroblasts and following serum stimulation of quiescent, density-inhibited chicken embryo fibroblasts. Active potassium transport and …
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Investigating the Functional Response of a Subsurface Biofilm Community to Xenobiotic Stress
… and the stress response glutathione-gated potassium efflux (GGKE), to benzene, pentachlorophenol (PCP), or Cd exposure were studied in up-flow sand columns to examine the contribution of each response to the overall functional response of a subsurface biofilm. Benzene was catabolized in the …
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Stimulus-Mitosis Coupling in the Rat Thymic Lymphocyte
… hundred-fold and was associated with heightened potassium exit. An enhanced potassium efflux has been observed in a variety of tissues consequent upon an elevation in the free cytosolic calcium concentration. Via this indicator, several hormonal and non-hormonal mitogens were found to increase …
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Chemical Inhibition of Nitrification: Evaluating Methods to Detect and Characterize Inhibition and the Role of Selected Stress Responses Upon Exposure to Oxidative and Hydrophobic Toxins
… we showed that a rapid (minutes) antioxidant potassium efflux mechanism does not exist in two nitrifying bacteria, Nitrosomonas europaea and Nitrospira moscoviensis. Furthermore, we showed that another important antioxidant molecule, glutathione, was not oxidized as readily as in a …
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The Development of a Bacterial Biosensor Designed to Detect Oxidative Chemicals in Water: Correlating Sensor Relevance to Mammalian Brain Cells and Assessing Bacterial Cell Immobilization Strategies
… bacteria called the glutathione-gated potassium efflux (GGKE) mechanism is a good biological indicator to be used in a biosensor designed to detect the presence of oxidative chemicals in water. The authors of this study propose the development of a GGKE biosensor using an environmental …
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Intracellular triggering of inflammation by the extracellular bacterium Pseudomonas aeruginosa
… activation is also dependent on extracellular potassium. An increase of extracellular potassium leads to a complete abrogation of inflammasome activation by P.aeruginosa and Salmonella. To further elucidate this finding, we investigated the leakiness of the pore formed by the T3SS in the host …
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Adrenergic-cholinergic interactions in the heart
… is related to the ability of carbachol to open potassium channels. In rabbit left atria, carbachol exerted a direct negative inotropic response and inhibited the positive inotropic response to phenylephrine. Carbachol also promoted the efflux of 86 Rb in the presence and absence of phenylephrine …
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Elucidating the Role of Toxin-Induced Microbial Stress Responses in Biological Wastewater Treatment Process Upset
… glutathione-gated, electrophile-induced potassium efflux system is responsible for deflocculation observed due to shock loads of toxic electrophilic (thiol reactive) chemicals. The results indicate significant K+ efflux from the activated sludge floc structure to the bulk liquid in …