Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 32 for “"post-translational regulation"”.
-
Post-Translational Regulation of Zeb1 Contributes to Tgfβ-Mediated Emt
… (TGF-β) receiving attention for its up-regulation in the metastatic tumor microenvironment. TGF-β facilitates EMT through a variety of mechanisms but a prominent feature of TGF-β induced EMT is the activation of the transcription factor zinc finger E-box-binding homeobox 1 (Zeb1). …
-
Post-translational regulation of BiP by FICD-mediated AMPylation and deAMPylation
Regulation of the amount and activity of Binding Immunoglobulin Protein (BiP) contributes to protein-folding homeostasis. BiP’s abundance is modulated transcriptionally by the canonical unfolded protein response (UPR). Conversely, a metazoan-specific, endoplasmic reticulum (ER)-resident, Fic domain …
-
Post-translational Regulation of Plant Fatty Acid Desaturases as Expressed in Saccharomyces cerevisiae
… than BF3. These differences are attributed to post-translational modification and have been shown to be controlled by an Nterminal element. I attempted to determine specific amino acids important for regulation, and further characterize the mechanism contributing to the differences. Through …
-
Novel insights into the post-translational regulation of an ER localised ubiquitin conjugating enzyme
Ube2J1 is an enzyme that helps cells degrade misfolded proteins at the endoplasmic reticulum. This study shows it is controlled by several signalling pathways, and regulated by previously unidentified mechanisms. Ube2J1 also boosts replication of Vesicular Stomatitis Virus when active. These …
-
Post Translational Regulation of AID Targeting to Both Strands of a Transcribed DNA Substrate
… underscores the importance of understanding its regulation throughout the genome. Action of AID on genes outside of the Ig loci can lead to genomic instability. Hyperactivity of AID has been shown to cause chromosomal translocations and other oncogenic malignancies. Loss of AID can lead to …
-
Transport activity dependent regulation of the yeast general amino acid permease
… when amino acids are abundant by two distinct post-translational mechanisms. Gap1p is regulated post-translationally to respond quickly and efficiently to changing amino acid concentration. An increase in amino acids causes accumulation of Gap1p in the vacuole and inactivation of Gap1p located …
-
The regulation of expression level in NeuroD/BETA2 by PKA
Post-translational regulation of bHLH transcription factors could be effected by phosphorylation, then NeuroD/BETA2 could be effected by phosphorylation too. NeuroD/BETA2 was phosphorylated at Ser336 by CaMKII, then formation of dendirte could be effected by phosphorylation of NeuroD/BETA2. …
-
Development of a single-cell fluorescence-based sensor to investigate Myc protein stability
… This pleiotropic activity renders loss of Myc regulation highly beneficial to tumour cells and, in fact, Myc is deregulated in most human cancers. While its contributions to cancer have made Myc a thoroughly studied protein and an extremely desirable drug target, over fifty years of research …
-
Phosphorylation Impairs Dicer1 Function to Accelerate Aging and Tumorigenesis In Vivo
… aging, and cancer in humans. Recently, post-translational regulation of Dicer1 via phosphorylation has been described in <em>C. elegans</em>. Oscillation of Dicer1 phosphorylation to regulate its activity is essential for germ cell development and embryogenesis in worms. These …
-
Understanding the roles for CELF2 in post-transcriptional regulation for the fate of neural stem cells.
… The fate of NSCs are achieved by intrinsic gene regulation along with extrinsic environmental factors. As gene regulation is an essential process for all cells, understanding its coordination is important to understand how the fate of cells are determined. Gene regulation can occur at three main …
-
Investigating the Post-Translational Modification of Notch1 Intracellular Domain: From Proteolytic Cleavage to Mass Spectrometric Profiling
… determination, cellular development and its dysregulation has been widely associated with cancer. Thus, post-translational modifications (PTM) further fine-tune Notch activity by modulating its trafficking, stability, and transcriptional dynamics. This thesis investigates the post-translational …
-
Mechanistic Studies of the Activation of Ubiquitin-Conjugating Enzymes by Ring-Type Ubiquitin Ligases
Ubiquitination, modification with ubiquitin, is a post-translational regulation of proteins in eukaryotes. Ubiquitin-activating enzymes (E1) activate ubiquitin and form thioester linkages with ubiquitin, which are then transferred onto ubiquitin-conjugating enzymes (E2). Ubiquitin-conjugating …
-
Jab1 Negatively Regulates Pten and Promotes Resistance to Trastuzumab In Her2-Positive Breast Cancer
… cancer. I demonstrated that Jab1 mediated the post-translational regulation of PTEN by associating with PTEN and facilitating its degradation. I also found that the C-terminal end of Jab1 was required for Jab1 to induce degradation of PTEN. Furthermore, I showed that proteasome inhibitors …
-
Interrogations of protein and RNA: localisation, interactions and dynamics
… RNAs and proteins are fine-tuned by a set of. post-transcriptional and post-translational regulation mechanisms fundamental to complex cellular processes, throughout the stages of development or in response to acute stimuli. Whereas microscopy- or affinity-based methods are routinely employed …
-
Characterization of Fe-S Cluster Assembly On the Sufbc2D Complex
… transcription factor IscR. While transcriptional regulation of the suf genes has been well characterized, almost nothing is known about Suf post-transcriptional or post- translational regulation. We show that recombinant SufBC2D proteins over-expressed in E. coli are phosphorylated at serine and …
-
The developmental role and regulation of the Anaphase-Promoting Complex/Cyclosome in Drosophila meiosis
… PIMPLES, the Drosophila homolog of Securin. Both post-transcriptional and post-translational regulation of cort result in expression of CORT protein being restricted to the meiotic divisions. Cytoplasmic polyadenylation of cort mRNA is tightly correlated with appearance of the protein in mature …
-
Tail-anchored Triphosphate Tunnel Metalloenzymes in Arabidopsis thaliana: An Investigation into the Molecular Mechanisms underlying their Biological Functions
… was governed by their transcriptional regulation. In this thesis, I show that the knockout mutant of TTM1 (ttm1) also displays delayed natural and ABA-induced senescence, indicating its universal role as a positive regulator of senescence-related PCD. I further found that TTM1 undergoes …
-
Analyses of the MEC1 DNA Damage Pathway in Saccharomyces cerevisiae
… Fundamental to the repair process is coordinated regulation of repair genes and initiation of cell cycle arrest protocols. Failure to preserve these checkpoints results in accumulation of mutated DNA and aberrant cell phenotypes that are characteristic of human disease. The yeast, Saccharomyces …
-
Peroxisomal beta-oxidation of fatty acids in neonatal swine
… to examine development, importance, nutritional regulation, and inducibility of peroxisomal $\beta$-oxidation (POX) of fatty acids in piglets. POX activity in liver, kidney, and heart, measured either by an antimycin/rotenone-insensitive incubation method or by palmitoyl-CoA dependent …
Page 1 of 2