Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"post-transcriptional modifications"”.
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The developmental liver transcriptome of Rattus norvegicus
Our research is involved in the transcriptional dynamics of perinatal liver development. The transcription factors and their downstream effects have been well studied in regards to early liver development, yet perinatal liver development is much less well understood. In an attempt to investigate …
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Investigating the potential of the Metastasis Associated Antigen 1 (MTA1) for cancer immunotherapy in a murine model
… at very low levels. Furthermore, to rule out any post-transcriptional modifications, MTA1 protein levels were also confirmed by western blotting. It was observed that most of the cell lines expressed MTA1 at high levels, whereas no protein expression was detected in the normal tissues by western …
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HAGE, a novel cancer/testis antigen with strong potential as a target for immunotherapy against cancers
… and in cell lines in order to rule out any post-transcriptional modifications. Furthermore, HAGE has been previously described as member of the DEAD-box family of ATP-dependent RNA helicases but very little is known about its actual function. RNA helicases are involved in various steps of …
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Cloning, sequencing, and biochemical characterization of endo-N-acylneuraminidase
… SDS-PAGE fractionated Endo-N indicates that no post-transcriptional modifications are necessary for expression of native protein. A method for detecting molecular weight variants, which could be indicative of in vivo protein folding intermediates as found in the P22 tail spike protein, is …
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Functional characterization of the RNA binding protein RALY
… 90% are subject to alternative splicing or other post-transcriptional modifications. All these events produce a high number of different proteins that form the basis for the high variety of cells. The RNAbinding proteins (RBPs) play crucial roles in this variability by regulating many steps of …
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Genomic approaches to understand sweetpotato root development in relation to abiotic factors
… protein kinases (CDPK) and genes involved in post-transcriptional modifications (protein phosphatase 2A and a phosphatase associated protein) were up-regulated in early developing storage roots in comparison to fibrous roots. Thirteen out of 20 selected genes showed altered expression under …
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GUIDE RNA-DEPENDENT AND INDEPENDENT tRNA MODIFICATIONS IN ARCHAEA
Stable RNAs undergo a wide variety of post-transcriptional modifications, that add to the functional repertoire of these molecules. Some of these modifications are catalyzed by stand-alone protein enzymes, while some others are catalyzed by RNA-protein complexes. tRNAs from all domains of life …
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A systems biology approach unravels the biological function of two tRNA wobble base modifications in fine-tuning translation and the response to environmental stress
tRNA molecules undergo extensive post-transcriptional modifications to produce several variations of the four canonical nucleotides. Despite the huge number of nucleotide modifications that have been identified in tRNAs, to date their biological roles and regulations continue to be poorly …
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Protein factors involved in the biogenesis of the mitochondrial ribosome
… ribosome requires both mt-rRNAs to undergo post-transcriptional chemical modifications, folding of the rRNA and assembly of the protein components assisted by numerous biogenesis factors. The post-transcriptional modifications of the mt-rRNAs include base methylations, 2’-O-ribose …
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Functional miRNA regulation of metastatic genes promotes tumor cell dissemination in non-small cell and small cell lung carcinomas
… emphasis on the role of noncoding RNAs in post-transcriptional modifications has revealed their important contribution in the regulation of developmental programs across metazoan species. More recently, the role of alterations in expression of small noncoding RNAs, microRNAs (miRNAs) has …
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STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF ARCHAEAL BOX H/ACA RIBONUCLEOPROTEIN INVOLVED IN RIBOSOMAL RNA PSEUDOURIDYLATION
Ribosomal RNAs (rRNA) undergo several post-transcriptional modifications inside the cell. These modifications can be (1) RNA- independent (enzyme only) and (2) guide RNA-mediated. In the latter mechanism, a group of small, metabolically stable, non-coding RNAs, present as ribonucleoprotein (RNP) …
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Characterization of splicing mechanisms by single-molecule fluorescence
… human U2 and U6 snRNAs contain a large number of post-transcriptional modifications. Recent studies have shown these modifications make human snRNAs more stable than that of yeast indicating a possibility of having different spliceosomal activation states.</p> <p> In order to understand and …
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A TALE OF TWO METHYLATION MODIFICATIONS IN ARCHAEAL RNAs
… all the three domains of life, most RNAs undergo post transcriptional modifications both on the bases as well as the ribose sugars of the individual ribonucleotides. 2'-O-methylation of ribose sugars and isomerization of Uridines to Pseudouridines are two most predominant modifications in rRNAs …
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Apobec1-Mediated RNA Editing in Monocytes Contributes to Genetic Heterogeneity and Modulates Monocyte Activity and Development
<p>Post-transcriptional modifications such as alternative splicing have been shown to add to the complexity needed to compensate for the relatively low number of genes found in higher organisms. Many other modifications recently found in mRNA, which cannot be deduced from what is coded in the …
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Elucidating the function and biogenesis of small non-coding RNAs using novel computational methods & machine learning.
… animal and plant hairpin precursors along with post-transcriptional terminal modifications that are not encoded by the genome. This method allows the characterisation of the prevalence of miRNA isoforms within different cell types and/or conditions. We have applied Chimira within a larger study …