Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"post-thaw motility"”.
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Effect of different equilibration periods pre-cryopreservation on post-thaw sperm motility in Nguni and Boran bulls
… of spermatozoa through the numerous freezing-thawing steps should be studied, in order to identify the optimal conditions for the survival of spermatozoa. The discovery of protective agents within egg yolk and glycerol was a major milestone in sperm cryopreservation. These agents protect …
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Evaluation of Stallion Frozen-Thawed Semen Using Conventional and Flow Cytometric Assays
Field evaluation of frozen-thawed stallion semen has been limited to tests such as post-thaw motility and morphology that are not only subjective but also evaluate only a small population of cells. Flow cytometry has provided a quick, repeatable, objective method of evaluating a large number of …
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The utilization of the prostacyclin analogue iloprost in the cryopreservation of stallion spermatozoa
… and development directed toward improving post thaw parameters. Most producers utilize glycerol as the primary cryoprotectant when freezing stallion semen, fully knowing that the cell undergoes significant damage during the cryopreservation process as well as from the exposure to the …
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Seminal plasma and freeze-thaw injury to bovine sperm
… of sperm exposure to seminal plasma on freeze-thaw injury. In Experiment I, 14 ejaculates from 10 Holstein bulls were held at 32°C for either 0, 20, 40, 60, 120 or 240 min followed by dilution in egg yolk-citrate. All treatments were maintained at 32°C for 240 min post-collection, at which time …
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Evaluation of Different Concentrations of Egg Yolk in Canine Frozen Semen Extender
… a commonly used cryoprotectant, by examining the motility and morphology throughout the freezing process: initial (baseline after extender added), post-cool (after three hours at 5"C) and post-thaw (after freezing.) Initial values of pH, osmolarity, motility and morphology were obtained for …
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Cryopreservation of microencapsulated bovine spermatozoa
… and evaluated at 0.5, 1, 2, 4, 8, and 16 h post-encapsulation. For sperm encapsulated there were no significant differences in sperm motility. However, peak time of maximum sperm release differed between PLL and PDL membranes at 2 and 4 h of incubation. In Trial 2, sperm viability and …
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Factors affecting post-thaw quality of cryopreserved boar sperm and its effect on gilt fertility
… resources. However, due to variability in post-thaw quality of sperm and decreased pregnancy rates and litter sizes, the swine industry has not been able to fully utilize the potential of frozen-thawed boar sperm (FTS). Two experiments were performed to evaluate factors affecting the …
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Comparison of epididymal and ejaculated sperm collected from the same Holstein bulls
… I, epididymal sperm was found to have higher post-thaw motility compared to ejaculated sperm. During cryopreservation, the membrane permeability of ejaculated and epididymal sperm was found to be similar. In addition, the membrane permeability of both ejaculated and epididymal sperm was …
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Factors affecting fertility in frozen-thawed boar sperm
Frozen-thawed boar sperm holds the potential to have an impact on the future of the swine industry. Utilization of this technology could improve a swine producer’s ability to access top-tier genetics from around the world, to improve efficiency, profitability, and the quality of product to meet …
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Determining the role of nerve growth factor-beta in seminal plasma on bovine reproduction
… prostate, vesicular glands) were harvested post-mortem from mature bulls and either frozen or formalin-fixed for Ngf gene expression and protein localization. Seminal plasma NGF concentrations were measured in the pre-ejaculate and sperm-rich fraction from bulls collected by …