Global ETD Search
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Showing 1 to 20 of 28 for “"post-column"”.
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Investigation of the Luminol Chemiluminescence Reaction as a Post-Column Reaction Detector for HPLC
… (CL) reaction has been used as a post-column reaction detector for high performance liquid chromatography (HPLC). The research reported here has progressed in two principle areas: first, the development of a method for the determination of amino acids and other complexing species …
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Investigations of Chemiluminescence Detection Parameters for Kinetic Analysis and Capillary Separations
A post-column CL detection method for capillary electrophoresis (CE) was developed in which the capillary effluent was collected on a membrane which had been coated with bis(2,4,6-trichlorophenyl)oxalate, a CL reagent. After the CE run, the CL reaction was initiated on the membrane by applying …
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The Determination of Trace Metals by High Performance Liquid Chromatography with Photometric Detection
… especially since the synthesis of better column packings. The adaptability of reverse phase and ion chromatography with new post-column reagents for photometric detection for the determination of transition metal cations, is discussed. Separation of divalent transition metal by high …
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DETERMINATION OF TRACE METALS BY lON-CHROMATOGRAPHY WITH CHEMILUMINESCENCE DETECTION
… area of the analytical procedure. Various post-column reagent systems, for trace metal analysis, based on the highly sensitive luminol-peroxide chemiluminescence (CL) reaction have been developed and are described. A simple cation-exchange chromatographic system was developed and used to …
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Development of HPLC Techniques for the Analysis of Trace Metal Species in the Primary Coolant of a Pressurised Water Reactor
… on Aminex A9 and Benson BC-XI0 analytical" columns. Detection was by post column reaction with Eriochrome Black T and Calmagite Linear calibrations of 2.5-100ng of cobalt (the main species of interest), were achieved using up to 200ml samples. The detection limit for a 200ml sample was 10pg …
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Detection of Biologically Important Molecules
… reagents and their application in an HPLC post-column system are described. Saccharides are widely used in industrial processes and biological systems. The determination of a specific saccharide is difficult since sugars are colorless and structurally similar to each other. Futhermore, the …
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Studies of tris(2,2'-bipyridyl)ruthenium(II) electrogenerated chemiluminescence for detection in flowing streams
… HPLC. The Ru(bpy)$\sb3\sp{2+}$ can be added to post-column effluent via a mixing-tee or the Ru(bpy)$\sb3\sp{2+}$ can be directly placed in the mobile phase. In the latter approach, the pump, tubing, and mixer necessary for post-column addition are eliminated although the retention times change. …
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THE DETERMINATION OF TRACE METALS IN CONCENTRATED BRINES FOR PROCESS CONTROL
… onto a dynamically coated chelating ion-exchange column. The column selectively retained the analytes of interest while allowing the matrix to pass through unchanged. This allowed the simultaneous preconcentration of the analytes and matrix removal. Following the preconcentration step, the …
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On-line screening of bioactive compounds and application of comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry for the analysis of birch bark
… chromatography separates complex mixtures, and a post-column biochemical assay determines the activity of the individual compounds present in the mixtures. At the same time, parallel chemical detection techniques (e.g., diode-array detection, mass spectrometry, and nuclear magnetic resonance) …
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Deoxyribose oxidation chemistry and endogenous DNA adducts
… residues from deoxyribose C4'-oxidation and HPLC/post-column derivatization methods were developed to quantify the corresponding base propenal or malondialdehyde.
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Derivation and chromatographic separation of acid indole auxins
… separation of these compounds, including column length, choice of liquid phase, and column temperature were studied and optimized. The effects of derivative storage were examined and optimum storage conditions, alone; with the stability of the primary reaction product, were determined. The …
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Utilización de señales fluorescentes para el análisis y caracterización de vinos: Mejora de la sensibilidad y selectividad mediante derivación fotoquímica
… has been successfully carried out in pre- and post-column configuration. Also, voltammetric techniques have been used for the first time for the determination of resveratrol in wine samples. Moreover, the possibilities of front-face fluorescence spectroscopic signals of wine are investigated, …
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Development of a detection technique for liquid chromatography and microbore liquid chromatography using tris(2,2'-bipyridyl)ruthenium(II) electrogenerated chemiluminescence
… the need for Ru(bpy)$\sb3\sp{2+}$ to be added post column or to the mobile phase. Oxalate, a biologically important compound responsible for several clinical disorders, was separated and quantitated from both urine and blood plasma samples. This was done using an ion-pair reverse-phase …
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THE FABRICATION AND STUDY OF METAL CHELATING STATIONARY PHASES FOR THE HIGH PERFORMANCE SEPARATION OF METAL IONS
… trace metals in complex samples, using a single column and isocratic elution, is described. Hydrophobic, neutral polystyrene divinylbenzene resins were either impregnated with chelating dyes or dynamically modified with heterocyclic organic acids, using physical adsorption and chemisorption …
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Synthesis and properties of novel macrocycles and related chromophoric benzenoid compounds
… for carbohydrates and the development of post-column selective reagents for the detection of mono- and oligosaccharides. This latter work is still in progress and the preliminary studies are described here. In another study involving macrocyclic architectures, tiliacorinine, an alkaloid, …
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Mass Spectrometric Analysis of Thiol Proteins/Peptides Following Selenamide Derivatization And Electrolytic Reduction of Disulfide Bonds
… derivatization viareactive DESI to solve the post-column derivatization problems, also integration with EC. In addition, reactive DESI, which exploits the potential for coupling specific ion/molecule reactions with the ionization event, greatly improves the selectivity and efficiency for …
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Assessment of coal liquefaction behavior through product characterization with hyphenated chromatographic/spectroscopic methods
… PTIR (RP-HPLC/PTIR) detection through on-line, post-column extraction was developed. Though intended for application to coal-liquefied product (CLP) analysis, this system was evaluated rigorously for both chromatographic and spectral performance. Throughout this investigation, the overall …
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The development of methods for the determination of selenium using spectrofluorimetric and ICP-MS techniques.
… prior to on-line photoreduction of Se (VI), and post column hydride generation before ICP-MS detection. Separation of the three species was achieved with excellent resolution in less than 18 minutes. The limit of determination for the three species was 0.50 mug L[-1].
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Improved mass spectrometry characterization of organic compounds using analyte preconcentration and radio-frequency ionization.
… of organic compounds with MS. A home-built post-column cryogenic trap (PCCT) coupled to a commercial gas chromatography (GC)/quadrupole MS system was utilized for VOC analysis. Analytical figures of merit, including limit of detection (LOD) and signal-to-noise ratio, for GC/PCCT/MS were …
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