Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"positional cloning"”.
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Positional cloning and characterization of the mouse pudgy locus
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1997.
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Positional Cloning of Adiposity Genes in Ethnic Minorities of the Insulin Resistance Atherosclerosis Family Study
… in the ethnic cohorts of the IRASFS through positional cloning of genes and genetic variants influencing intermediate adiposity phenotypes. Presented herein are descriptions of the methods used, and the results of, candidate adiposity gene analysis via comprehensive single nucleotide …
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Invisible to arbuscular mycorrhizal fungi: Positional cloning and characterisation of a novel arbuscular mycorrhizal mutant in Zea mays
… are exposed to WT and mutant plant exudates. Positional cloning methods have identified a deleted region in the ina genome containing three candidate genes that may be responsible for this phenotype. Although further research is required to confirm the gene behind the ina phenotype, it was …
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Approach to the identification of sex-determining genes in the tilapia genome by genetic mapping and comparative positional cloning
<p>Tilapia (Oreochromis species) are one of the most dominant species in the aquaculture market. Genomic approaches may contribute to tilapia culture in the near future by identifying genes controlling traits valuable at the market. One of the most important traits for farming of tilapia is sex …
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Positional cloning of nude, a fork head transcription factor : genetic, physical and transcriptional maps of the region and mutations in the mouse and rat
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.
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Genetic study of hematopoiesis development by two zebrafish mutants: Ugly duckling and tc-244
… had severe defects in definitive hematopoiesis. Positional cloning revealed that the tc-244 mutant phenotype was caused by a point mutation in another novel zebrafish gene. Thus, tc-244 is identified as an important regulator for definitive hematopoiesis.
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Axon guidance in the zebrafish visual system: Analysis of Esrom Mutant
… for retinal ganglion cell (RGC) targeting. Using positional cloning esrom was found to be an orthologue of PAM/Hiw/RPM-1. This large multidomain protein has an E3 ligase activity. Esrom is ubiquitously expressed in the embryo. RGCs require it for proper activation of p38 signal transduction …
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Zebrafish Mutagenesis Study of Host Determinants of Mycobacterial Infection Outcome
… genomic regions harbouring causative mutations. Positional cloning has been completed on one such mutant, Guaguancó which was found to map to a nonsense mutation in the gene ubiquitously expressed transcript (uxt), a poorly characterised gene not previously associated with anti-mycobacterial …
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Use of a conservation-of-linkage strategy to identify a candidate for the rat Lymphopenia gene
… (BB) rat. In order to overcome the lack of positional cloning resources available in the rat to clone the Lyp gene, we developed and successfully implemented a conservation-of-linkage (COL) cloning strategy. Using the COL between the rat Lyp region and its murine counterpart, we developed …
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A molecular-genetic study of Congenital Nystagmus
… study is followed by linkage analysis and positional cloning. A novel nystagmus gene is investigated in a large cohort of Congenital Idiopathic Nystagmus (CIN) patients and X-inactivation studies are performed. Subsequently, cell culture and RT-PCR work is performed to study expression of …
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Characterisation of Rare Genetic Variants Conferring Susceptibility to Psoriasis
… activity. In the second part of the study, a positional cloning approach was applied to the analysis of a multigenerational psoriasis pedigree, where the disease appeared to segregate as an autosomal dominant trait. Tentative evidence for linkage (LOD > -2) was obtained for two regions on …
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New signalling network in plant abiotic stress discovered through a genetic approach
… mapping of the mutations will lead to the positional cloning of the affected genes, identifying new elements involved in the Arabidopsis abiotic stress signalling cascades.
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Understanding the genetic and physiological control of pre-harvest sprouting and pre-maturity amylase in UK wheat
… above, I also undertook the fine-mapping and positional cloning of the 4A QTL (named Phs), as this QTL showed the highest effect on PHS resistance of all the QTL studied. I took advantage of recent advances in wheat genomics, high-throughput genotyping and the syntenic relationship between …
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Preliminary quantitative trait loci analysis for biomass traits in Miscanthus sinensis
… analysis sets the foundation for fine mapping, positional cloning, and the development of a marker assisted selection program in the ongoing effort to improve Miscanthus as a biofuel crop.
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Characterization of xtcl mutant with reduced cuticular wax accumulation
… components were reduced to a similar extent. Compositional analyses of leaf and seed fatty acids demonstrated that saturated fatty acid content was decreased by around 55%, and unsaturated fatty acid content was approximately 20% lower in xtcl mutants. A detailed examination of xtcl seeds …
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Cloning and Characterization of the dominant Inhibitor of Wax 1 (Iw1) gene in polyploid wheat
… The aim of this study was to identify Iw1 via a positional cloning approach and to characterize its effects on yield and green-canopy duration in a field-grown set of glaucous and non-glaucous Near Isogenic Lines (NILs). In addition a number of physiological experiments were carried out on these …
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Molecular analysis of the sex-determining region of the Y chromosome in the platyfish Xiphophorus maculatus
… centiMorgans. This situation should allow the positional cloning of the sex-determining gene (SD) of the platyfish. For this purpose, Bacterial Artificial Chromosome (BAC) contigs were assembled from a BAC library of XY males constructed in our laboratory, using the oncogene Xmrk, egfrb, as …
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Forward screening for herbicide resistance in Arabidopsis thaliana and the monocot species Triticum aestivum
… identified to cause weak indaziflam resistance. Positional cloning coupled with next-generation sequencing revealed that a point mutation in the CULLIN1 (CUL1) encoding gene caused the decrease in indaziflam sensitivity. CUL1 is an E3 ligase involved in a variety of plant signalling pathways, …
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Forward and reverse genetic approaches to investigate cellulose biosynthesis in Physcomitrium patens
… resistance was applied on P. patens, and positional cloning combined with next-generation sequencing revealed two point mutations in CULLIN1 (CUL1) and AUXIN/INDOLE-3-ACETIC ACID INDUCED (Aux/IAA) which both are involved in auxin signaling pathways. The mutants were also cross-resistant to …
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Early liver formation in zebrafish: a molecular and morphological approach
… addition distinct defects in pancreas formation. Positional cloning of the s436 line identified a novel allele of histone deacetylase 1 (hdac1), which plays distinct roles in endodermal organogenesis in zebrafish. Loss of Hdac1 causes defects in timely liver specification and subsequent …
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