Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"porins"”.
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Kristallstruktur eines mycobacteriellen Porins
Ziel der Arbeit war die Aufklärung der dreidimensionalen Struktur des Porin A aus Mycobacterium smegmatis (MspA). Ausgehend von einem bekannten Reinigungsprotokoll wurde versucht durch Mutationen die Kristallqualität der ungenügend-streuenden Wildtyp-Kristalle zu verbessern. Durch die Einführung …
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Characterization of β-lactamases and Porins in Uropathogenic E. coli
… and KPC and the protein production of the porins, OmpC and OmpF in E. coli clinical isolates were characterized. It was hypothesized that increased β-lactamase production and/or outer membrane porin aberrations would result in elevated β-lactam minimum inhibitory concentrations. There were …
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Regulation of Outer Membrane Porins in P. aeruginosa Strains Collected from Patients with Cystic Fibrosis
… been associated with specific outer membrane porins of P. aeruginosa and their ability to affect cell structure and stability, adhesion to surfaces, and transport of small molecules. However, little is known about how P. aeruginosa is able to adapt and persist within the CF airways. In part, …
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Impact of environmental conditions on expression levels of resistance-nodulation-division (RND) efflux pumps and outer membrane porins in Acinetobacter baumannii
… three RND efflux pumps and three outer membrane porins of A. baumannii. In addition, we investigated the difference in expression levels of the selected two-component systems between a type strain of A. baumannii, ATCC 19606, and two clinical isolates with the MDR and biofilm-forming phenotypes. …
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Pseudomonas aeruginosa OprD-Family Porins Facilitate Imipenem Translocation Resulting in AmpC β-Lactamase Induction Contributing to the Effectiveness of Imipenem/Relebactam
… Third, to determine the contribution other OccD porins: OpdP, OpdB, and OpdB in the translocation of imipenem into the cell in the absence of OprD. Method: A panel of laboratory strains of P. aeruginosa and various clinical isolates were evaluated. Methods used in this dissertation included …
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Ionic Control of Porin Permeability
Porins are transmembrane proteins within the outer membrane of gram-negative bacteria that allow nutrients, and small molecules, including antibiotics, to enter the cell. Electrophysiological studies on porins reconstituted in lipid bilayers have suggested a dynamic regulation of porin conductance …
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Beyond MICS: Determining the Role of Porin Production and β-Lactamase Expression in Resistance and Cellular Physiology of Klebsiella pneumoniae
… and downregulation of outer membrane porins. However, the extent to which these mechanisms interplay in a resistant phenotype is not well understood. β-lactamase expression has long been presumed to have a previously unidentified fitness cost. In fact, little is known about how …
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Uncovering Mechanisms of Cell Envelope Integrity and Nutrient Acquisition in Acinetobacter baumannii
… study, we characterize a family of OM porins required for growth on carboxylates. The dicarboxylic acid porin (DcaP) was putatively assigned dicarboxylate specificity based on genomic context. We show that major A. baumannii vaccine candidate DcaP3 is important for growth on …
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Elucidation of the osmoregulatory locus, ompRZ, in Erwinia chrysanthemi
… regulator, OmpR. OmpR, in turn, regulates the porins, OmpF and OmpC in a reciprocal manner, so that one porin predominates over the other, depending on osmotic conditions. Erwinia chrysanthemi, which causes "soft rot" in a wide range of economically important crops, has been demonstrated to …
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Outer Membrane Porin Regulation among Different Sequence Types of Escherichia coli Clinical Isolates
… plasmid-encoded AmpC. Altering the production of porins could impact the fitness, and therefore the success of ST131 E. coli. The experiments described in this dissertation sought to identify differences in the regulation of the OmpC and OmpF porins among different E. coli sequence types by …
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Rational Design of DNA-Based Lipid Membrane Pores
… the diversity of nature - from ion channels to porins.
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Carbohydrate-Interacting Proteins from Two Nostoc (Cyanobacteria) Species
… belong to a family of (putative) cyanobacterial porins. Proteins in this family possess features that include a N-terminal signal sequence, a single SLH domain motif, followed by a coiled-coil region, and a C-terminal region that is homologous to the b-barrel-forming region of bacterial porins. …
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Computational methods for transport properties
… thesis can permeate the membrane crossing some porins (beta barrel proteins nestled in bacterial outer membrane) so the permeation happens when we observe a transport of the antibiotic through a porin. In this thesis we will focus on some computational methods, which are suitable to increase our …
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Enhanced sampling methods and their application in the study of molecular permeation in gram-negative bacteria
… in the study of molecular permeation through porins, overcoming the intrinsic limitations of both experimental techniques and standard molecular dynamics. The first test-case is Acinetobacter baumannii’s CarO. The use of substrates with varying charge and molecular weight, as well as the …
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Characterising the immune response to Salmonella and Salmonella surface antigens during a systemic infection
… contrasts with the potent benefit conferred by porins. These proteins induce, and can mediate protection through a T-independent B1b cell population. In particular, antibody to OmpD is key for this protection. These results suggest that vaccines that induce protective antibody to STm may be more …
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Monte Carlo Simulation of Nanostructures: Semiconductor Devices to Ion Channels
… many of the properties exhibited by OmpF porins. We found that the large negative charge on the porin molecule was mainly responsible for the highly selective nature of porin channels. The channel currents obtained from our simulations matched experimental data for a range of bias …
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Molecular characterization of the starch utilitization system of Bacteroides thetaiotaomicron
… the breakdown products of enzymes through porins, Bacteroides spp. appear first to bind the polysaccharide at the cell surface and then to transport the molecule into the periplasm, where the majority of the polysaccharide-hydrolyzing enzymes are located. Nonetheless, the preponderance of …
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Channel-forming proteins in the cell wall of amino acid-producing Corynebacteria
… genes that are encoded by the minus strand. Both porins are cotranscribed and coexist in the cell wall, which was demonstrated in RT-PCR and immunological detection experiments. The arrangement of porHC.glut and porAC.glut on the chromosome is similar to that of porBC.glut and porCC.glut and it …
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A Proteomics Approach to the Examination of Proteins in Marine Systems
… proteins identified were responsible for porins, transport, binding, translation, and protein refolding and could represent potential biomarkers of bacterial processes and/or activity. A parallel study of <italic>R. pomeroyi</italic>, in which <super>13</super>C-labeled leucine was added …
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Investigation of stability, dynamics and scope of application of mycobacterial porin MspA: a highly versatile biomolecular resource
… channel protein and is one of the most stable porins known to date. MspA has been successfully isolated and purified to obtain liquid extracts and crystals using a modified extraction procedure. A full analytical assessment has been carried out to authenticate its’ structure, including gel …
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