Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"porin"”.
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Ionic Control of Porin Permeability
Porins are transmembrane proteins within the outer membrane of gram-negative bacteria that allow nutrients, and small molecules, including antibiotics, to enter the cell. Electrophysiological studies on porins reconstituted in lipid bilayers have suggested a dynamic regulation of porin conductance …
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Outer Membrane Porin Regulation among Different Sequence Types of Escherichia coli Clinical Isolates
… the permeability of its outer membrane through porin downregulation, contributing to β-lactam resistance. Aberrations in permeability correlate with decreased carbapenem susceptibility when the organism produces an extended-spectrum β-lactamase or plasmid-encoded AmpC. Altering the production of …
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Structural and spectroscopic studies on the porin-cytochrome complex from Shewanella oneidensis MR-1
The"outer"membrane,"hetero!trimeric"multi!heme"cytochrome"complex"MtrCAB,"enables"the" process"of"dissimilatory"metal"reduction"(DMR)"in"Shewanella(oneidensis." "" The"properties"of" the"decaheme"protein"MtrA"as"well"as"a" truncated"version"of" this"protein" were" investigated" using" analytical" …
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Investigation of stability, dynamics and scope of application of mycobacterial porin MspA: a highly versatile biomolecular resource
Porin A from Mycobacterial smegmatis (MspA) is an octameric trans-membrane channel protein and is one of the most stable porins known to date. MspA has been successfully isolated and purified to obtain liquid extracts and crystals using a modified extraction procedure. A full analytical assessment …
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Beyond MICS: Determining the Role of Porin Production and β-Lactamase Expression in Resistance and Cellular Physiology of Klebsiella pneumoniae
… and downregulation of outer membrane porins. However, the extent to which these mechanisms interplay in a resistant phenotype is not well understood. β-lactamase expression has long been presumed to have a previously unidentified fitness cost. In fact, little is known about how …
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Monte Carlo Simulation of Nanostructures: Semiconductor Devices to Ion Channels
… were performed for the gramicidin and OmpF porin channels. The large size of chloride ions and electrostatic factors were found to be the main reasons for the absolute selectivity shown toward sodium ions by gramicidin channel. The gramicidin channel currents correlated very well with the …
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A Major Cell Envelope Protein Of Escherichia Coli K12: Purification And Properties
… The binding was dependent on the presence of porin and normal K12 LPS. Mutants that lacked porin, or had shortened LPS were unable to bind SEP or transport succinate. SEP binding to porin immobilized on Sepharose was also demonstrated. This binding was very specific in the presence of low …
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Engineered Vesicles for Perchlorate Degradation
… vesicle functionalized with the outer membrane porin OmpF. The porin allows for perchlorate transport into the vesicles, inside which the encapsulated enzymes transform perchlorate to chloride. Perchlorate reduction was quantified using a methyl viologen colorimetric technique. The vesicle …
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Puuvillateollisuuden yhteistyö ja kilpailu vuosina 1903 - 1939
… yksi Suomen kuudesta puuvillatehtaasta, Porin Puuvilla Oy. Tämä synnytti uudenlaisen kilpailutilanteen; kotimaan markkinoista kilpailivat PMK, Porin tehdas sekä jatkuvasti lisääntyvä ulkomainen tuonti. Porin tehdas liittyi syndikaattiin kolmen ankaran kilpailuvuoden jälkeen vuonna 1935. …
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Characterization of β-lactamases and Porins in Uropathogenic E. coli
… extended spectrum β-lactamases, AmpC cephalosporinases, and serine-carbapanemases. Decreased drug influx due to outer membrane porin defects can confer resistance to the carbapenems when paired with the production of the ESBLs and AmpCs. In this study, the mRNA and protein expression of the …
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Regulation of Outer Membrane Porins in P. aeruginosa Strains Collected from Patients with Cystic Fibrosis
… been associated with specific outer membrane porins of P. aeruginosa and their ability to affect cell structure and stability, adhesion to surfaces, and transport of small molecules. However, little is known about how P. aeruginosa is able to adapt and persist within the CF airways. In part, …
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Elucidation of the osmoregulatory locus, ompRZ, in Erwinia chrysanthemi
… regulator, OmpR. OmpR, in turn, regulates the porins, OmpF and OmpC in a reciprocal manner, so that one porin predominates over the other, depending on osmotic conditions. Erwinia chrysanthemi, which causes "soft rot" in a wide range of economically important crops, has been demonstrated to …
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Biogenese von mitochondrialen beta-Barrel-Membranproteinen
… von mitochondrialen beta-Barrel-Proteinen, wie Porin und Tom40, benötigt. In Eukaryoten kommen beta-Barrel-Membranproteine nur in der Außenmembran von Mitochondrien und Chloroplasten vor; in Prokaryoten nur in der Außenmembran von Gram-negativen Bakterien. Die essenzielle Untereinheit des …
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Kristallstruktur eines mycobacteriellen Porins
… Aufklärung der dreidimensionalen Struktur des Porin A aus Mycobacterium smegmatis (MspA). Ausgehend von einem bekannten Reinigungsprotokoll wurde versucht durch Mutationen die Kristallqualität der ungenügend-streuenden Wildtyp-Kristalle zu verbessern. Durch die Einführung der Mutation A96R …
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The evolution of the autoantibody response in Graves’ disease
… in the periphery may be associated with the porin proteins of Y. enterocolitica.
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Pseudomonas aeruginosa OprD-Family Porins Facilitate Imipenem Translocation Resulting in AmpC β-Lactamase Induction Contributing to the Effectiveness of Imipenem/Relebactam
… been tied to the depletion of the outer membrane porin, OprD and overproduction of AmpC chromosomal β-lactamase. It is not known how relebactam potentiates imipenem when AmpC levels are low, nor how imipenem traffics into the cell in the absence of OprD. The purpose of this dissertation was to …
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Identification and recombinant production of antimicrobial peptides from the Black Soldier Fly, Hermetia illucens (L.)
… structure similar to a Gram-negative bacterial porin. Genes encoding the 9 candidate putative AMPs were cloned into a pET expression system that was under the control of the inducible T7 promoter. Recombinant proteins were heterologously expressed and purified from Escherichia coli BL21(DE3) …
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Uncovering Mechanisms of Cell Envelope Integrity and Nutrient Acquisition in Acinetobacter baumannii
… study, we characterize a family of OM porins required for growth on carboxylates. The dicarboxylic acid porin (DcaP) was putatively assigned dicarboxylate specificity based on genomic context. We show that major A. baumannii vaccine candidate DcaP3 is important for growth on …
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Protein glycosylation in the gram-negative gamma proteobacterium photorhabdus luminescens
… using mass spectrometry to be an outer membrane porin, OmpN. No glycoproteins have previously been identified in P. luminescens. The ompN gene was cloned and expressed in both Escherichia coli and P. luminescens. It was found that OmpN purified from P. luminescens was capable of binding the …
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Computational methods for transport properties
… thesis can permeate the membrane crossing some porins (beta barrel proteins nestled in bacterial outer membrane) so the permeation happens when we observe a transport of the antibiotic through a porin. In this thesis we will focus on some computational methods, which are suitable to increase our …
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