Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"pore forming protein"”.
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Dynamics and Model of the Pore-Forming Protein Lysenin
<p>Membrane transporters are a class of membrane proteins that function to provide a pathway across a cell membrane for the movement of ions and biomolecules. Investigations into the regulatory mechanism of these systems are hindered by their extensive preparation requirements compounded by their …
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The role of a pore-forming protein, Mpeg1, in cytosolic import
… The main aim of my PhD was to identify proteins that play a role in the cytosolic import of antigens during cross-presentation. To do so we developed a new assay, which relies on the type I ribosome inactivating protein, saporin, which can inhibit translation upon entry into the cytosol. …
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Molecular Interactions of Human C9: The Pore Forming Protein of the Complement System
… non-enzymatic assembly of C5b, C6, C7, C8, C9 forming C5b-9<sub>n</sub> (n=12-18), a cytolytically active pore-like structure known as the membrane attack complex (MAC). C8 is a heterotrimer comprised of a disulfide linked C8α-γ dimer that is non-covalently associated with C8β. Following …
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Characterization Of Streptolysin O As The Translocator For The Cytolysin-Mediated Translocation Process In Streptococcus Pyogenes
… <italic>Streptococcus pyogenes</italic> uses the pore-forming protein <underline>s</underline>trepto<underline>l</underline>ysin <underline>O</underline>: SLO) to translocate the <italic><underline>S.</underline></italic> <italic><underline>p</underline>yogenes</italic> …
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Investigating the role of TspA, a novel substrate of the <i>Staphylococcus aureus</i> Type VII protein secretion system
… was used to identify candidate T7 substrate proteins. A previously uncharacterised protein, SAOUHSC_00584, was found in the culture supernatant in a T7-dependent manner. Cell fractionation and protease accessibility experiments demonstrated that SAOUHSC_00584 is a peripheral membrane protein …
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Atypical Mechanisms of Synthesis, Activation and Release of Interleukin-1β from Vascular Smooth Muscle Cells
… to high levels inside VSMCs. VSMCs expressed the pore-forming protein Gasdermin D, and the active form of Gasdermin D permeabilised VSMCs, yet mature IL-1β was still not released. Finally, mature IL-1β extracted from VSMCs has 13-fold lower activity than equal amounts of mature IL-1β from Thp1 …
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VDAC and SOD1: two major players in mitochondrial metabolism and in ALS
… work presented here has been devoted to two proteins, the Voltage-Dependent Anion Channel (VDAC) and the Superoxide Dismutase I (SOD1) and has been especially focused on the relationships between them in physiological or pathological conditions of the cell. VDAC is a pore-forming protein …
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Deciphering the regulation of perforin-2-mediated endocytic escape in dendritic cells
… endocytic compartments from where internalised proteins can escape into the cytosol for proteasome-mediated generation of MHC-I-binding peptides. Prior to my project, the Kozik lab had identified the pore-forming protein perforin-2 as a dedicated effector of endocytic escape that allows …
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Characterization of endocytic escape pathway and its role in adaptive immunity
… cytosol, and further processed like endogenous proteins for presentation on MHC class I. Endocytic escape of antigens appears to be the rate-limiting step of cross-presentation, yet we know very little about the molecular mechanisms involved. To identify the machinery involved in endocytic …
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Reversibly neutralized perfringolysin O for intracellular delivery of macromolecules/
… bases of disease, macromolecules such as proteins and siRNA can potentially be used as therapeutics, modulating biological function with high specificity to reverse pathological progression. However, while certain contexts require manipulating the biology inside the cell, the large size …
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Structure-based development of novel chemical scaffolds as inhibitors of the store-operated calcium entry pathway
… or pharmacologically. SOCE is mediated by Orai proteins, mainly by Orai1, the pore-forming protein of the CRAC (Calcium release-activated calcium) channel, which is activated following store-depletion by aggregated stromal interaction molecule (STIM) proteins that are localised within the …
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Human Complement Protein C8: Crystal Structure and Analysis of Binding Interactions
… on bacterial cell membranes to form a lethal pore-like structure. The MAC is comprised of complement proteins C5b, C6, C7, C8 and C9. The 150-kDa C8 protein consists of three non-identical subunits (C8alpha, C8beta, C8gamma), arranged as a disulfide linked C8alpha-gamma heterodimer …
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Isolation and characterization of channel-forming proteins in the outer membrane of E. coli and Borrelia species
In this study pore forming proteins of the gram-negative bacteria B. burgdorferi, B. duttonii and E.coli were investigated. Therefore the study is subdivided into three parts. In the first part outer membrane preparation of three relapsing fever Borrelia were investigated. In the second part the …
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Channel-forming proteins in the cell wall of amino acid-producing Corynebacteria
… thesis was to identify and study novel channel-forming proteins of the amino acid producers C. glutamicum, C. callunae and C. efficiens. Cell wall extracts of the organisms were investigated and a novel pore-forming protein, named PorH, that is homologue in all three organisms, was detected and …