Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"polytene chromosomes"”.
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Localization of Small-Nuclear-Rna Genes of Drosophila Melanogaster on Polytene Chromosomes
The existence of discrete, stable, small nuclear RNAs (snRNAs) is known since 1960s. These RNAs range in size from 90-300 nucleotides and are confined to eukaryotic nuclei. snRNAs have been well-characterized biochemically and have been isolated and sequenced from different organisms. Recent …
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Pressure Treatment of Polytene Chromosomes and Computer Vision Techniques for High Resolution Cytogenetic Studies
… by the interphase chromatin organization in polytene chromosomes has made Drosophila the premier model organism for analysis of chromosome structure and behavior. A major impediment to full exploitation of polytene chromosome cytology has been the difficulty in producing high quality …
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Colocalization between Akirin, the NuRD Complex, and Histone Modifications
… deacetylase (NuRD) complex. <em>Drosophila</em> polytene chromosomes were used to visually identify colocalization between Akirin and subunits of the NuRD complex. The NuRD subunit, HDAC1, functions to remove acetyl groups from histone tails. Therefore, the colocalization between Akirin and …
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Genetic and cytological investigation of Nucleostemin-1 in Drosophila melanogaster
… several transcriptionally active inter-bands of polytene chromosomes. Over-expression caused melanotic tumors and larval and pupal lethality. RNAi depletion of NS1 caused a loss of imaginal (precursor) cells in the larval midgut, and an apparent block in the nucleolar release of large ribosomal …
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Liquid-on-liquid mixing for slide-based biological assays
… immunofluorescence staining of Drosophila larval polytene chromosomes by a factor of 100 in time and concentration, when compared to standard coverslip techniques. Flow visualization experiments reveal the fluid motions in the thin aqueous layer. Using time-lapse video photography to monitor the …
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Optimization of Cytogenetic and Physical mapping of Culicinae genomes
… Aedes and Culex genomes, the structure of their polytene chromosomes is damaged by ectopic contacts that make the analysis difficult. Mitotic chromosomes from imaginal discs of 4th instar larvae of Aedes aegypti were tested as a source for the physical genome mapping for this mosquito. …
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Evidence that the Boundary Element-Associated Factors BEAF-32A and BEAF-32B affect chromatin structure in Drosophila melanogaster
… BEAF-32B bind to hundreds of loci on Drosophila chromosomes. These proteins function as insulators; they can prevent promoter activation by an enhancer when placed between them and protect transgenes from chromosomal position effects. To gain insight into BEAF function we designed and expressed a …
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Characterization of Boundary Element-Associated Factors BEAF-32A and BEAF-32B and Identification of Novel Interaction Partners in Drosophila Melanogaster
… (FRAP) to assess the dynamics of BEAF-32 on polytene chromosomes and also observed banding patterns, with the help of fluorescent protein labels, and evaluated its behavior during mitosis in early embryos. Finally, results obtained with BEAF prompted us to test for physical interactions …
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The genetic and molecular analysis of the mfs(2)31 locus in Drosophila melanogaster : a novel suppressor of position-effect variegation
… An in situhybridization to salivary gland polytene chromosomes revealed a P element in distal 31E of this strain. The P element, located in distal 31E, was cloned from thedysgenic mfs(2)31 allele. This element is responsible for the reduced transcription of a 1.2 kb message which is …
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Comparative genomics of chromosomal rearrangements in malaria mosquitoes
… and whole genome amplification of polytene chromosomal fragments to obtain DNA for sequencing and assembly. The chromosomal regions spanning both breakpoints of the 2La in A. arabiensis and A. merus were laser microdissected from the polytene chromosomes. Subsequently, DNA samples …
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Tracking translation factors in fission yeast nucleus
… of Drosophila melanogaster (D. melanogaster) polytene chromosomes. Whilst these findings might indicate the presence of ribosomal subunits at transcription sites, it has also been reported that these proteins associate with non-coding RNA in Saccharomyces cerevisiae (S. cerevisiae), suggesting …
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Chromosome evolution and mechanisms of speciation in the Anopheles gambiae complex
… with unpaired and insufficiently condensed chromosomes. As a result, primary spermatocytes undergo a mitosis-like anaphase division, producing nonmotile and malfunctional diploid sperm with two tails. However, individuals can mate with females normally and form the mating plug to induce the …
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The organization and evolution of heterochromatin in the Anopheles gambiae complex
… Morphologically, some regions of An. gambiae chromosomes exhibited characteristics similar to transcriptionally active puffs or peri-centromeric heterochromatin. We characterize these regions, as well as the rest of the genomic landscape, by using morphological and genetic features to identify …
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ΜΕΛΕΤΗ ΤΩΝ ΠΟΛΥΤΑΙΝΙΚΩΝ ΧΡΩΜΟΣΩΜΑΤΩΝ ΤΩΝ ΚΥΤΤΑΡΩΝ ΤΩΝ ΣΙΑΛΟΓΟΝΩΝ ΑΔΕΝΩΝ ΤΗΣ DROSOPHILA AURARIA
… DROSOPHILA SPECIES IN THAT THEIR SALIVARY GLAND CHROMOSOMES EXHIBIT BALBIANI RING (BRS). WE PRESENT HERE A CYTOLOGICAL MAP OF D. AURARIA AND INFORMATION ON THE DEVELOPMENTAL PROFILES OF ITS PUFFS AND BRS. AUTORADIOGRAPHY HAS BEEN PERFORMED IN ORDER TO CONFIRM THE RNA SYNTHESIS OF THE BRS DURING …
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The subnuclear localisation of Notch responsive genes
Title: The subnuclear localisation of Notch responsive genes. Candidate Name: Matthew Jones Notch signalling is a highly conserved cell-cell communication pathway with critical roles in metazoan development and mutations in Notch pathway components are implicated in many types of cancer. Notch is …