Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"polyplexes"”.
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Multifunctional bioreducible nanoparticles for gene therapy
… ability remain major challenges. Bioreducible polyplexes (polyelectrolyte complexes of disulfide-containing polycations and nucleic acids) utilize the redox potential gradient existing between extracellular space and intracellular environment as a physiological stimulus to enhance delivery of …
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Cationic Glycopolymers for DNA Delivery: Cellular Internalization Mechanisms and Biological Characterization
… removal leads to decreased uptake. Individual polyplexes appear to have differing affinities for specific GAGs, as polyplex dissociation occurs in a charge-independent manner, and may influence binding. Internalization occurs through close interactions with GAGs, as GAGs accumulate on polyplex …
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Understanding Non-viral Nucleic Acid Delivery Vehicles with Different Charge Centers and Degradation Profiles
… release of polyethylenimine (PEI) based polyplexes, which could contribute to high transfection efficiency. Secondary amine structures rendered PGAAs buffering capacity around physiological pH. To test the feasibility of the mechanism for PGAAs, new no buffering capacity guanidine or …
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Novel Lanthanide Containing Polymers for Nucleic Acid Delivery and Monitoring of Polyplex Dynamics
… Dynamic light scattering performed on the polyplexes (polymer-DNA complexes) indicate that they are in nanometer size range (50-80nm). All the polymers used to form polyplexes exhibited low toxicity to cultured human Glioblastoma cells (U-87) and showed variable transfection efficiency …
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Complexation Between DNA and Hydrophilic-Cationic Diblock Copolymers
DNA-polycation complexes (polyplexes) are gene delivery vehicles that offer a number of advantages over viral vehicles: high genetic payload capacity, low toxicity, and low cost. Among the many polycation architectures, hydrophilic-cationic diblock copolymers are particularly promising due to their …
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Endocytosis mechanism of poly- and lipoplexes in siRNA delivery in HeLa cells
… lipid- or polymer based particles (lipoplexes or polyplexes). The materials that are used as the delivery vectors can mediate the uptake and the release of siRNA in the cytosol. In order to synthesize newer generation of the delivery materials, it is paramount to know through which endocytosis …
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Elucidation of Design Criteria for siRNA Delivery in Mammalian Cells Using Polyethylenimine
… escape, and (3) unpackaging of PEI/siRNA polyplexes. Chapter 2 will demonstrate that high knockdown efficiency can be achieved when appropriate amount of branched 25-kDa PEI is used despite PEI/siRNA ratio if the minimum amount of siRNA is met. It will also examine how PEI concentration …
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Modulatable endosomalytic, intracellularly biodegradable vectors for gene delivery
… sizes, surface charges and the stability of RPC polyplexes under the simulated physiological conditions extra- and intracellularly suggested that these RPCs are promising vectors. In addition, the transfections revealed that the RPCs can facilitate endosomal buffering and intracellular reduction …
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Characterization of Intermolecular Interactions in Nanostructured Materials
… binding ratio of linear polyethylenimine and DNA polyplexes as determined by a novel NMR method. Dynamic light scattering (DLS) and zeta potential measurements were also performed to determine the size and surface charge of polyplexes. Circular dichroism (CD) and FTIR spectroscopies provided …
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Engineering of Nonviral Vectors for Gene Therapy
… analog of human insulin to the cyclodextrin polyplexes, I was able demonstrate a 2--3 fold enhancement in transfection efficiency in insulin receptor-rich cell lines.
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Development of Carbohydrate-based Diblock Polymers for Nucleic Acid Delivery
… was utilized to freeze-dry siRNA containing polyplexes which could be re-dissolved in water after lyophilization to yield nanoparticles. The polyplexes formulated with cationic diblock copolymers were shown to efficiently enter cervical cancer cells (HeLa cell line) and glioblastoma cells …
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Non-covalent Intermolecular Interactions in Polymer Design: Segmented Copolymers to Non-viral Gene Delivery Vectors
… varied in the charge density were measured for polyplexes formed with poly(galactaramidopentaethylenetetramine) (G4) cationic polymers and pDNA. Results showed that the affinity of GAGs for G4 polyplexes was not completely dependent on the electrostatic interactions indicating that other factors …
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Synthesis and Characterization of Nucleobase-Containing Polyelectrolytes for Gene Delivery
… to high degree of cationic character for the polyplexes formed with these systems according to the proton-sponge hypothesis. One method of reducing the overall cationic character for these systems is incorporation of non-electrostatic binding mechanisms such as hydrogen bonding. A series of …
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Design, development, and evaluation of novel polymer-drug conjugates and interpolymer complexes for the treatment of neurodegenerative disease.
… study aimed to develop novel PDCs and their nano-polyplexes as multifunctional therapeutic agents for AD treatment. Vanillin, an antioxidant, was conjugated to polyallylamine hydrochloride (NM10 and NM15), while the cholinesterase inhibitor naphthalimidohexylamine (HEXNAP) was conjugated to …
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Development of polymer and lipid materials for enhanced delivery of nucleic acids and proteins
… uptake and trafficking mechanisms of PBAE/DNA polyplexes, and demonstrate that polyplex internalization and transfection depend on a key endo/lysosomal cholesterol transport protein, Niemann-Pick C1 (Npcl). Finally, working with cationic lipids termed lipidoids, which have shown exceptional …
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Mechanistic Study of DNA Delivery from Self-Assembled Nanolayered Films
… we identified relative polymer to DNA content in polyplexes as a key driver of effective transfection. We then explored the impact of LbL assembly parameters on DNA multilayer film composition and release kinetics, and how these subsequently influence transfection efficacy in vitro. Finally, we …
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Exploring new applications for photophysically encoded microcarriers
… We have shown that, compared to PEI based polyplexes, they were less efficient in transfecting cells. However, as they had very low toxicity, further tailoring of the nature and extent of cationic side chains on cationic hydroxyethylcellulose may be a promising avenue to further enhance …
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Effect of uptake pathway on non-viral gene delivery in vitro and in vivo
… weight, or zeta potential of the PEI-derivative polyplexes, caveolin-dependent endocytosis was critical to effective gene delivery. In order to further understand the impact of endocytic pathways on gene delivery efficiency in vitro, we modified HeLa and MDA-MB-231 cells to express small hairpin …
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Specific and efficient in vivo delivery of DNA and siRNA by polyethylenimine and its derivatives
… and evaluated for its ability to target PEI-9B9 polyplexes following intravenous delivery in a rat. Although mAb 9B9 retains affinity for its substrate ACE, PEI-9B9 does not enhance delivery to its intended target, the lung. Clearance of PEI-9B9 from circulation likely occurs before antibody …
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Development of therapeutic agents for myotonic dystrophy type 1
… myotubes. In addition, the oligomer can form polyplexes with a locked nucleic acid (LNA) CAG16 gapmer, facilitating its cell uptake in both HeLa cells and DM1 patient myotubes, with a resulting decrease in the level of toxic r(CUG)exp significantly. RNA-seq analysis indicated that the …
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