Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"polypeptide chain"”.
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A Computational Framework for Interacting with Physical Molecular Models of the Polypeptide Chain
… of a flexible, scaled, physical model of the polypeptide chain, which accurately reproduces the bond rotational degrees-of-freedom in the peptide backbone. The coarse-grained backbone model consists of repeating amide and alpha-carbon units, connected by mechanical bonds (corresponding to phi …
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Factors Affecting Translation of Messenger RNA's In Vitro: Use of a GTP Analogue to Investigate Rates of Polypeptide Chain Elongation
<p>The order of addition of amino acids to a growing protein is determined by the codon sequence of a messenger RNA molecule. This translation process was studied in vitro with a cell-free protein synthesis system derived from Escherichia coli. The rate of protein synthesis was proportional to the …
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Studies of T4 Polynucleotide 5'kinase 3'phosphatase
… be found for having both activities on the same polypeptide chain. In addition, little is known concerning the location of the active sites on the polypeptide chain or if the two activities share a single active site.
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A novel function for the eukaryotic translation elongation factor 1A
… to this indirect interaction with the nascent polypeptide chain it is shown here for the first time that eEF1A also associates directly with the nascent polypetide chain distal to the peptidyl transferase center of the ribosome. This is demonstrated for a variety of nascent polypeptide chains …
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Studies on the Flavin Binding Properties of Native and Protease-Activated Pyruvate Oxidase From Escherichia Coli
… reactivation appears to be the folding of the polypeptide chain in the monomer-FAD complex to reconstitute the three dimensional FAD binding site prior to subunit reassociation. The subsequent formation of native tetramers appears to proceed via an essentially irreversible dimer assembly …
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The nature and role of oxidation state dependent conformational differences in cytochrome c
… states were identified. Three segments of polypeptide chain, located for the most part on the Met80 side of the protein, were shown to display an increase in mobility in the oxidized state. A conserved internal water molecule, Wat166, was observed to shift 1.7 A towards the heme iron atom …
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Immunochemical studies with tryptic peptides of tobacco mosaic virus protein
… are situated in the C-terminal region of the polypeptide chain of the TMV protein, residue156 being associated with the cryptotope and residue 158 with the neotope. No antigenic activity could be demonstrated in tryptic peptide I I (residues 135-141 ). When the results are analyzed in terms of …
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A study of SecA : the motor of the bacterial secretion system by site-directed spin labeling and EPR
… with several entities: unfolded precursor polypeptides, the molecular chaperone SecB, membrane phospholipids, and the membrane-embedded translocase SecYEG. Functional studies implied that some of these interactions occurred simultaneously. However, little is known about the details of these …
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Human Serum Albumin Characterisation and Binding Studies by Spectroscopic Techniques
… and exogenous ligands, having a single polypeptide chain of 585 amino acids constructed in three domains of relatively equal size. Although HSA's existence has been known for many years, its characterisation and ability to bind ligands still remain enigmatic. Only in 1992 was the crystal …
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Mechanistic studies of the AAA+ molecular motor ClpXP
… peptide substrates to probe what features of a polypeptide chain are recognized during translocation. Surprisingly, side-chain properties including size and charge, and the spacing between peptide bonds had relatively small effects on the rates of translocation by ClpXP. Pulling on tracts of …
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Isolation and characterisation of histone transacetylases
… and tryptic digestion as the intact histone H4 polypeptide chain is blocked at its N-terminus. The Edman degradations of histone H3 and H4 showed that the acetylation sites that have been determined under in vitro conditions are the same as those undergoing acetylation in vivo. All of the …
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Energetics of [alpha]-helix formation in peptides and proteins
… the co-translational folding of the nascent polypeptide chain during protein biogenesis in vivo. The energetics of helical propensities have been studied intensively over the last four decades in both peptides and proteins, but fundamental controversies remain to date, as essential …
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Chain dynamical theories of protein folding
… of protein folding must take into account chain dynamics. The energy landscape description of protein folding accommodates two rather distinct behaviors of the polypeptide chain: the glassy dynamics expected for heteropolymers with random interactions and the organized dynamics expected for …
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Structure and regulation of yeast glycogen synthase
… amino acids near the C-terminus of the enzyme’s polypeptide chain. We studied isoform-2 of yeast glycogen synthase as a model to study the structural and molecular mechanisms that underlie the regulation of the eukaryotic enzymes and our primary tools of investigation were macromolecular X-ray …
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The role of buried stack residues in the folding of the beta-helix domain of P22 tailspike
… parallel alignment of similar or identical side chains is a common structural phenomenon in amyloid fibers. In the crystal structure of an amyloid fiber, these residues not only align, but take identical side chain orientations, creating long stacks of identical residues with identical …
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Order and Disorder in Protein Biomaterial Design
… components to be precisely mixed within a single polypeptide chain. This dissertation reports the successful design and application of a new class of recombinant materials inspired by the protein elastin, termed partially ordered polymers (POPs), to uncover the impact of single chain interactions …
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Characterization of the regulatory domain of the mammalian multifunctional De Novo pyrimidne biosynthetic enzyme CAD
… (DHOase) are carried on a single 240-kDa polypeptide chain called CAD. Carbamoyl phosphate synthetase (CPSase), the first enzyme in the pathway catalyzes the synthesis of carbamoyl phosphate from two moles of ATP, bicarbonate, and ammonia derived from the hydrolysis of glutamine. The …
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Inhomogeneous Totally Asymmetric Simple Exclusion Processes: Simulations, Theory and Application to Protein Synthesis
… template (mRNA) and assemble amino acids into a polypeptide chain which folds into a functioning protein product. The ribosomes perform discrete directed motion that is well modeled by a totally asymmetric simple exclusion process (TASEP) with open boundaries. We incorporate the essential …
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PLP-Dependent α-Oxoamine Synthases: Phylogenetic Analysis, Structural Plasticity, and Structure-Function Studies on 5-Aminolevulinate Synthase
… circularly permuted ALAS variants and the single polypeptide chain ALAS dimer (ALAS/ALAS) exhibiting a greater turnover number than wild-type ALAS. An examination of the contribution of each ALAS/ALAS active site to the enzymatic activity shows that each active site makes distinct contributions to …
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Evolution of copper-containing nitrite reductase
… with a modest number of alterations to the polypeptide chain. With the aid of my newly developed high-throughput screening technique and site-directed mutagenesis, the mononuclear NiR active site was remodelled into a trinuclear Cu site similar to that of MCO. A crystal structure of this …
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