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Showing 1 to 20 of 20 for “"polymorphic loci"”.
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Isolation and Characterization of Polymorphic Loci from the Caribbean Flamingo (Phoenicopterus ruber ruber): New Tools for Wildlife Management
… microsatellite analysis. Seven new polymorphic loci were isolated from a microsatellite-enriched Caribbean flamingo genomic library and used to characterize survey populations of Caribbean and African greater flamingos. In addition, four of these loci were used to verify parentage …
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The Distribution of Genetic Variation Among Relictual Populations of Missouri Tallgrass Prairie Plants
… Fst estimates were 0.0318 for S. scoparius (two polymorphic loci), 0.0385 for R. humilis (six polymorphic loci), and 0.0242 for L. pycnostachya (seven polymorphic loci). thus, over 96% of the allozyme variation present within each of these species in Missouri is found with individual populations. …
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Genic identity and differentiation of Ilyodon (Cyprinodontoidea: goodeidae): morphological polymorphism and chromosomal divergence
… between the presumed species in seven to fifteen polymorphic loci, but substantial interpopulation genic differentiation. These results suggested regular gene flow between the supposed species at each of four independent localities and conspecificity of two mouth width morphs. Progeny of field …
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Relationships Between Genetic and Ecological Variables in Nine Populations of Phlox Drummondii Hook (Niche, Allozymes, Genetic Differentiation)
… by examining allozyme variability at five polymorphic loci and by analysis of morphological variation among families of full and half sibs. Statistically significant heritabilities were found for 16 morphological and life history characters, as well as significant differences among …
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Population substructuring in Schreibers' long-fingered bat (Miniopterus schreibersii) in South Africa
… (GA)0 microsatellite repeats. Five novel, highly polymorphic loci were identified. These five loci, and an existing mammalian microsatellite locus, were amplified in. 301 individuals, sampled from ten colonies throughout South Africa. Significant genetic heterogeneity exists within the M. …
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Genetic marker frequency differences among strains of goldfish, Carassius auratus
… mean heterozygosity (0.0% - 4.4%) and percent polymorphic loci (0.0% - 30.6%) within strains were low compared to other vertebrate species. Analysis of the isozyme data using Chi-square tests against Hardy-Weinberg genotype expectations and wright's (1943, 1951) F statistics indicated modest …
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Development of DNA markers and the genetic analysis of seven populations of the freshwater mussel Lampsilis radiata siliquoidea (Bivalvia: Unionidae) in north central Minnesota
… in north central Minnesota. Random amplified polymorphic DNA (RAPD), heteroduplex, and single-stranded conformational polymorphism (SSCP) analyses were performed using nuclear DNA markers. We assayed the populations for variation using a single repetitive 18 base pair RAPD primer and two loci …
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Spatial Genetic Structure in the Mount Vernon Prairie Population of Rudbeckia Hirta
… Analysis of spatial structure was based on three polymorphic loci (PGI-1, LAP-1) assayed by cellulose acetate electrophoresis. Significant spatial structure was evident for PGI-1 and LAP-1, but not for AAT-1. At the two loci with significant spatial structure, the scale of structure was extremely …
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An Assessment of Potential False Positive E.coli Pyroprints in the CPLOP Database
… By simultaneously pyrosequencing multiple polymorphic loci within the ribosomal operons known as the intergenic transcribed spacers, a reproducible output is obtained, known as a pyroprint, which can be used like a fingerprint to identify that organism. This section of the genome not only …
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GENETIC STRUCTURE OF PINUS STROBUS L BASED ON FOLIAR ISOZYMES FROM 27 PROVENANCES
… by starch gel electrophoresis. Twenty-one loci, five fixed, coding for 12 enzyme systems, including three invariants, were used for this study. Indirect evidence for genetic control of these loci was discussed.</p><p>Substantial genetic differentiation within and among provenances was …
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Analysis of Variant Alleles of Gene Thought to Affect Breast Cancer in a Patient Population from South Carolina
… RFLP and sequencing were used to assay 17 polymorphic loci in 629 breast cancer patients from South Carolina recruited between the years 2004-2008. Loci were selected based on studies in the literature suggesting that these loci might be associated with breast cancer or with aggressive …
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Population Structure of Lake Whitefish (Coregonus Clupeaformis) on Multiple Spatial Scales: Transitioning to a Genomics Approach
… resulted in adequate production of variable SNP loci, with higher sequencing depth (~12X) and larger numbers of polymorphic loci (~10,000) providing the best resolution of both broad and fine scale population differentiation analyses. Conservative bioinformatics parameters enabled retention of …
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Geographic distribution of southern- and northern-form brook trout populations in southwestern Virginia
… staining techniques. Variation at four polymorphic loci, including the diagnostic creatine kinase (<i>CK-A2*</i>) locus, was quantified in terms of genetic diversity and population genetic differentiation. Allele frequencies indicated that 19 populations were of putative southern origin, …
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GENETIC AND QUANTITATIVE VARIATION IN WILD SOYBEAN (GLYCINE SOJA) POPULATIONS
… by gel electrophoresis. Based on a total of 43 loci of 15 enzymes and one protein, an average of 67.4% polymorphism (at 99% level) was observed. The expected heterozygosity is 0.160, and the number of alleles per locus is 2.14. Genetic purity of these seed accessions are very high.</p><p>The …
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Uniparental disomy as a cause for congenital malformations and or developmental delay in inherited apparently balanced chromosomal rearrangements
… using DNA probes that detect highly polymorphic loci which have been previously mapped to these chromosomes [Nakamura et al. 1987,Lathrop et al. 1988, Nakamura et al. 1988, Boerwinkle et al. 1989,Batanian 1990, Standen et al. 1990, Weber et al. 1991, Zoghbi et al. 1991, Ranum et al. …
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Molecular marker analysis of a segregating monoploid potato family
… Simple sequence repeat analysis with eight polymorphic primer pairs was used successfully to identify the homozygous diploid plants that were added to the monoploids. In total 34 monoploid plants and 14 homozugous diploids were obtained. The degree of heterozygosity revealed by SSR analysis …
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Living apart together
… data for seven newly discovered polymorphic loci. Across eight social units and three breeding seasons, pairs were the prevailing grouping pattern (18 of 21 family years). Most pairs were stable for more than three mating seasons and used well defined stable territories. Although …
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Hybridization in Ants
… species, since PCR primers for microsatellite loci are often not useful outside the species for which they were designed. I identified 45 conserved microsatellite loci based on the eight ant genomes that were available at the time and designed primers for PCR amplification. Among these loci, I …
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Landscape genetics and CWD in white-tailed deer
… and redesigned primers for two of these loci. Analytical corrections for null alleles were unable to fully prevent bias associated with these genotyping errors, and consequently, measures of population differentiation and kinship were negatively impacted. Our results demonstrate the …
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Patterns of association in a co-introduced insect herbivore and parasitoid wasp
… range of the U.S. Thirty-four candidate loci were identified, 12 of which were ultimately chosen for detailed screening. Seven of the 12 loci were polymorphic, but only 5 of the 7 were successfully amplified across samples and ranges. Of these 5 polymorphic loci, there were 3 - 9 alleles …