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Showing 1 to 20 of 139 for “"polymerases"”.
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Subunit Compositions of Arabidopsis DNA-Dependent RNA Polymerases and the Roles of the Plant-Specific RNA Polymerases IV and V in Gene Silencing
In addition to RNA polymerases I, II and III, the essential RNA polymerases present in all eukaryotes, plants have two additional nuclear RNA polymerases, abbreviated as Pol IV and Pol V, that play non-redundant roles in siRNA-directed DNA methylation and gene silencing. Using a combination of …
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Do RNA-dependent polymerases share common ancestry? A bioinformatic approach
… (OSM) among two groups of RNA-dependent polymerases, the large (L) protein from viruses in the order Mononegavirales and the reverse transcriptase (RT) protein from retroviruses and retroid agents. An OSM was not detected among the L and RT proteins, suggesting that they are not homologs. …
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Genetic and Biochemical Properties of Arabidopsis RNA Polymerases IV and V
RNA Polymerases IV and V: Pol IV and Pol V) are plant-specific enzyme complexes with subunit homology to RNA Polymerase II: Pol II). The largest subunits in Pol IV and Pol V, NRPD1 and NRPE1 respectively, share a second largest subunit, NRPD2/NRPE2. The evolutionarily conserved Metal A and Metal B …
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The Role of Rna and Rna Polymerases in The Aging Process
Radioactive uracil incorporated into the total RNA of old Rhizoctonia solani cells was twofold less than that in young cells, but there was no change in RNA label following a chase with unlabeled uracil. As the concentration of labeled uracil in the medium increased, the amount of uracil label in …
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Novel function and regulation of mutagenic DNA polymerases in Escherichia coli
… dinB+ encode novel, low to moderate fidelity DNA polymerases with the ability to catalyze synthesis on imperfect DNA templates in a process termed translesion synthesis (TLS). Similar enzymes exist in nearly all organisms, constituting the Y-superfamily of DNA polymerases. Although DinB is the …
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Role of TLS Polymerases in Replication of DNA Lesions in Human Cells
… basis of lesion bypass and the role of TLS polymerases will result in insights on how human cells handle exposure to environmental carcinogens and how the TLS processes contribute to cancer avoidance or to cancer risk.
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Role of translesion DNA polymerases in mutagenesis and DNA damage tolerance in Pseudomonads
Kahjustused DNA-s, mis tekivad kas rakkude normaalse elutegevuse käigus või erinevate keskonnategurite mõjul (näiteks UV-kiirgus, DNA-d kahjustavad kemikaalid), pärsivad genoomi replikatsiooni, takistades replikatiivse DNA polümeraasi edasiliikumist. Pikaajaline replikatsiooni seiskumine võib …
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A computational approach for identification and development of novel inhibitors targeting viral polymerases
… and is the focus of this thesis. Herein, polymerases of three positive strand RNA viruses, viz HCV, BVDV and HIV have been targeted with the goal of improving the efficacy of antivirals against wide range of resistant mutations. Lack of effective therapies for these viral infections as …
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In Vitro Studies of Deoxyribonucleic Acid Polymerases Ii and Iii From Escherichia Coli
Made available in DSpace on 2014-12-14T07:14:41Z (GMT). No. of bitstreams: 1 7606786.pdf: 4678002 bytes, checksum: 25dac31f696bfb87b1e3e6b6200f914a (MD5) Previous issue date: 1975
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Functional specialization of Arabidopsis poly(A) polymerases in relation to flowering time and stress
… encode several copies of canonical poly(A) polymerases (cPAPs). The disruption of cPAPs in mammals results in lethality. In plants, reduced cPAP activity is non-lethal. Arabidopsis encodes three nuclear cPAPs, PAPS1, PAPS2 and PAPS4, which are constitutively expressed throughout the plant. …
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Lesion Bypass of N2-ethylguanine by the Human Y Family DNA Polymerases Iota and Kappa
… test the hypothesis that the human Y family DNA polymerases have a biological function in the bypass of alkylation DNA damage. The lesion bypass properties of the Y family DNA polymerases ι and κ opposite the N2-ethylGua DNA adduct were studied using steady-state and rapid kinetics and structural …
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Design, synthesis and evaluation of novel intercalator analogs targeting therapeutically important polymerases and folded RNA
Polymerases and ribonucleic acids have been found to play major roles in the causation and progression of several diseases. Targeting such important therapeutic targets using small molecules can help achieve their specific recognition and inhibition thereby intercepting key stages involved in the …
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Solution Conformations and Structural Thermodynamics of Type I DNA Polymerases from Escherichia coli and Thermus aquaticus
… (elongated or compact) for the full-length polymerases, and the binding mode (“polymerization” versus “editing”) of the DNA substrate when bound to Klentaq and Klenow. Comparisons of experimental and structure-based data indicate that full-length Pol I and Taq in solution adopt a …
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DNA structural selectivity of binding by the Pol I DNA polymerases from Escherichia coli and Thermus aquaticus
… Klentaq, the “large fragments” of the Pol I DNA polymerases from <I>Escherichia coli</I> and <I>Thermus aquaticus</I>, are considered functional homologues. Klentaq, however, does not have a functional proofreading site. Examination of the DNA binding thermodynamics of Klenow and Klentaq to …
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Gene expression in plastids of higher plants: evolutionary and functional aspects of different RNA polymerases - coordinated assembly of multiprotein-complexes
… both prokaryotic (PEP) and eukaryotic (NEP) RNA polymerases. In order to understand, how far the transcriptional apparatus within plastids was adapted to nuclear needs, three approaches have been undertaken. Firstly, the tobacco homologues of the NEP-enzymes known from Arabidopsis were determined …
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Single-molecule studies of bacterial DNA replication and translesion synthesis
… its genome, all cells employ replicative DNA polymerases that have intrinsic high fidelity. When an error occurs on the template DNA strand, in the form of lesions caused by diverse chemicals, reactive oxygen species, or UV light, the high-fidelity replicative DNA polymerases are stalled. To …
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Translesion synthesis and mutations: On the mutagenic properties of the two DNA lesions, 8-oxo-G and Pt-GG, and the functions of Y-family DNA polymerases and Rev3L on the bypass of each of the DNA lesions in mammalian cells
… with selective gene knockouts of distinct DNA polymerases as models, I deduced the biological functions of the translesion DNA polymerases Pol eta, Pol kappa, Pol iota, Rev1 and Rev3L on bypassing each of the lesions 8-oxo-G and Pt-GG. My study takes advantage of the Next Generation Sequencing …
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