Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"polyketide synthases"”.
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Type III Polyketide Synthases: Discovery, Characterization, and Engineering
The polyketides are a diverse group of natural products with important applications in medicine and industry. Industry, especially the pharmaceutical industry, is under pressure to deliver "greener" chemical syntheses that are less environmentally damaging and incorporate renewable resources. There …
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Cyclization modes in type III polyketide synthases and the synthetic compounds used as probes for mechanistic studies.
Type III polyketide synthases (PKS) catalyze different cyclization reactions of linear polyketide intermediates to produce polyketides with distinct ring structures. The mechanisms of pyrone and resorcinol ring formation in type III PKSs remain unknown, despite many years of research on this family …
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Studies Towards the Discovery of Novel Natural Products Through Functional Analysis of Environmental DNA-Encoded Type II Polyketide Synthases
… natural products, synthesized by Type II polyketide synthases (PKSs) in soil-borne bacteria. Through the functional characterization of soil eDNA-derived Type II PKS containing clones, herein we attempt to identify novel PKS systems that encode structurally diverse metabolites and explore …
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Dual-omics approach for 3,4-dihydroisocoumarin characterisation from Hydrangea serrata and isolation of polyketide synthases for heterologous expression in Pseudomonas putida KT2440
… superfamily, a group of plant specific Type III polyketide synthases. Chalcone synthases and their homologues, the stilbene synthases produce a huge amount of specialised plant metabolites including phenylpropanoids. The dihydroisocoumarin biosynthesis is assumed to be enhanced by elicitors and …
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STRUCTURE DETERMINATION, BIOCHEMICAL CHARACTERIZATION, AND PROTEIN ENGINEERING OF THE ACYL CARRIER PROTEIN DOMAIN FROM 6-DEOXYERYTHRONOLIDE B SYNTHASE
Polyketides are an important class of natural products. 6-Deoxyerythronolide B Synthase (DEBS) is a type 1 polyketide synthase that produces the Polyketide antibiotic drug erythromycin. DEBS is a large multi-domain enzyme complex containing multiple enzymatic domains, including acyl carrier protein …
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Detection and Purification of a Novel Natural Inhibitory Compound from an Isolated Strain of <em>Rhodococcus</em> Using an Agar Extraction Method
… the presence of 24 non-ribosomal peptide synthases and 7 polyketide synthases possibly involved in production of secondary metabolites. The use of a solid agar extraction method to screen soil isolates of <em>Rhodococcus</em> for compounds with inhibitory activity against other bacteria …
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Dearomative hydroboration-enabled synthesis of idarubicinone and synthesis of minor cannabinoids and their metabolites
… representatives of tetracyclic type II polyketide natural products that are widely used in cancer chemotherapy. Although syntheses of this class of compounds have been extensively explored, all known approaches are based on annulations, relying on the union of properly pre-functionalized …
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Characterizaion and Chemical Rescure of the Phenotype of ors-3, a Knockout mutant line of PpORS in Physcomitrella patens
… patens (PpORS) is basal to all plant type III polyketide synthases in phylogenetic trees, and may resemble closely their most recent common ancestor. PpORS knockouts were previously generated and partially characterized (Li et al., Planta, 2018, 247: 527–541). This study aimed to investigate …
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The Role of the Alternaria Secondary Metabolite Alternariol in Inflammation
… metabolites that are synthesized by genes called polyketide synthases (PKS) in immune responses of lung epithelial cells.
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Functional Analysis of Secondary Metabolite Biosynthesis-Related Genes in Alternaria brassicicola
… nonribosomal peptide synthetases (NPSs) and 10 polyketide synthases (PKSs) in the A. brassicicola genome. The phenotype resulting from knockout mutations of each PKS and NPS gene was investigated with an emphasis on discovery of fungal virulence factors. A highly efficient gene disruption method …
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Characterisation of microbial Mat communities in meltwater ponds of the McMurdo ice shelf, Antarctica
… non-ribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) genes were identified which could be the genetic basis for novel bioactives. The use of a multi-disciplinary approach synthesis and subsequent results significantly increased our understanding of the diversity and function …
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Characterization of chalcone synthase genes in red raspberry cultivar (rubus idaeus)
… of anthocyanin compounds involves type III polyketide synthases (PKSs): chalcone synthase (CHS), which is the first enzyme of the flavonoid synthetic pathways leading to synthesis of various anthocyanins. The European red raspberries are a commercially important fruit that needed both for …
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Studies of new natural products and their biosynthesis spearheaded by high-resolution mass spectrometry
… by non-ribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). In NRPS and PKS biosynthesis, monomer units are incorporated into a growing natural product chain in an assembly line fashion using a covalent thiotemplate mechanism. Because the growing natural product is covalently …
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Comparative genomics of bacteria from amphibian skin associated with inhibition of an amphibian fungal pathogen Batrachochytrium dendrobatidis
… activity were siderophores (N=17) and Type III polyketide synthases (N=20). We then analyzed the isolates belonging to the phylum Proteobacteria in more detail. We identified 197 gene families that were present in all 22 Proteobacteria. We examined various subsets of the Proteobacteria for genes …
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Analysis of the diverse antibacterial strategies used by <i>Serratia marcescens</i> Db10
… peptide synthetases (NRPS) and polyketide synthases (PKS) are widespread in bacteria and fungi and are responsible for the biosynthesis of many metabolites with medically relevant properties, including antibiotics. The activity of NRPS and PKS enzymes is dependent on their …
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Efforts Toward Production of Novel Natural Products From Uncultured Soil Microbes
… non-ribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). PPtases are responsible for the post-translational modification of NRPSs and PKSs, which are genes commonly seen in secondary metabolite biosynthesis. This strategy was used to enrich an eDNA-derived metagenomic library …
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Characterisation of Epicoccum spp. secondary metabolism genes through de novo genome and transcriptome sequencing
… the genome, including eight clusters containing polyketide synthases (PKS), which were predicted to be required for epipyrone biosynthesis. Protocols for epipyrone production by submerged fermentation of Epicoccum were optimised to compare two conditions that strongly differ by level of epipyrone …
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Natural product discovery and engineering at the protein, pathway, and genome scales
… demonstrate a genome-mining platform for fungal polyketide synthases via the one-step assembly of expression-ready plasmids by homologous recombination in yeast. Through the evaluation of previously uncharacterized endogenous promoters from Saccharomyces cerevisiae, we demonstrate the …
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Molecular dissection of Alternaria brassicicola pathogenesis mechanisms
… genes such as: ABC transporters, MAP kinases, polyketide synthases, serine proteases, and various plant cell wall degrading enzymes. One of the genes identified in all of the libraries had significant homology with the major Alternaria allergen Alt a 1. Phylogenetic analysis of Alt a 1 homologs …
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Discovering novel natural products from bioactive plants and bacteria
… One group of valuable natural products is polyketides, which are known to be antiviral, antibacterial, and anticancer. In my first project, I attempted to develop an efficient strategy for cloning and characterizing Type III PKS genes that are likely responsible for the synthesis of novel …