Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 8 of 8 for “"polyketide synthase (PKS)"”.
-
Biosynthetic characterisation of secondary metabolites from stromatolite cyanobacteria Symploca sp. 1013
… metabolites are formed due to the presence of polyketide synthase (PKS) or non-ribosomal peptide synthetise (NRPS). Bioactivities in areas such as antibacterial, antifungal and anticancer have been implicated in many of these metabolites. In the present study, various marine cyanobacterial …
-
Fatty acid metabolism of marine microalgae
… of Schizochytrium which was shown to employ a polyketide synthase (PKS) complex for primary DHA biosynthesis. Additionally, genes of the Schizochytrium PUFA-PKS were successfully expressed in transgenic yeast, which produced DHA. Candidates for genes encoding C. cohnii PUFA-PKS components and …
-
Investigation of Specialized Metabolites in Apicomplexan Parasite Life Cycles
… parasites, we have begun to characterize a polyketide synthase (PKS) with unknown resultant products. Together, this work provides the basis for understanding how specialized metabolites within the mosquito microbiome affect the Plasmodium parasite transmission capacity while also …
-
Studies on the biosynthesis of oudenone and verucopeptin
… ( 89 ) is structurally characterized by a polyketide-derived tetrahydropyranyl side chain and a 19-membered cyclodepsipeptide. A DNA fragment of 72.5 kb was isolated from a cosmid library of Actinomadura verrucosospora chromosomal DNA. Partial analysis and sequencing of this gene cluster …
-
Characterizing and Engineering Thioesterases as Biocatalysts for Macrocyclization
… Finally, the first extensive engineering of a polyketide synthase (PKS) TE was undertaken via a rational protein design approach and an LCMS-based screening method. Four variants were identified with improved macrocyclization activity over wild-type (WT), highlighting the utility and success of …
-
Estudio genómico, transcriptómico y proteómico del proceso de infección del hongo fitopatógeno botrytis cinerea
… control. In the first chapter, the genes BcPKS2, BcPKS7 and BcPKS20, responsible for encoding polyketide synthase (PKS) enzymes in B. cinerea, were examined, with an initial focus on the production of botrycinereic acid. However, the generation of knock-out mutants for these genes did not …
-
Novel Molecules from Environmental DNA-Derived Type II Polyketide Biosynthetic Gene Clusters
… microbes have been found to be rich in type II polyketide synthase (PKS) gene clusters (Kieser, Bibb et al. 2000). PKS gene clusters produce a structurally diverse collection of aromatic natural products. The nascent polyketide in these pathways is produced by a conserved minimal PKS, composed …
-
Investigating and engineering the post-translational modifications in RiPP natural product biosynthesis (I) Thiopeptides (II) Sactipeptides
… Many of these natural products are derived from polyketide synthase (PKS) or non-ribosomal peptide synthetase (NRPS) families, including well-known antibiotics such as the beta-lactams, tetracyclines, macrolides, and glycopeptides. With the rising resistance to these proven antibiotic classes, …