Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"polyclonal antiserum"”.
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Identification of an aqueous glue protein, SCP-2, and the development of a polyclonal antiserum against the bHLH transcription factor SGSF in Latrodectus Hesperus
… proteins were injected into rabbits and the polyclonal antiserum was collected and tested by western blot analysis to determine the specificity of the immunological reagent. Western blot analyses revealed the anti-SGSF antiserum was capable of recognizing bacterially expressed SGSF in an …
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Expression and Functional Analysis of Hepatitis B Virus DNA Polymerase
… the nature of HBV DNA polymerase. Also rabbit polyclonal antiserum specific for each domain of HBV DNA polymerase was produced. RNAs of chimeric DNA polymerases, and 2.4 and 2.1 Kbp of surface RNAs were produced by all chimeras but the expression proteins of chimeric polymerases were not …
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Characterication of aggregate gland silk factor 1
… hesperus. After the development of an anti-AgSF1 polyclonal antiserum, we demonstrate by western blot analyses that the AgSF1 protein is highly expressed in the aggregate gland and the protein is localized to the connection joints of cobweaver webs. We also overexpress and purify two different …
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Immuno-gluorescence study of five zygomycetous fungi with two chitin-binding probes
A polyclonal antiserum was prepared against a purified microsomal chitinase isolated from the fungus Choanephora cucurbitarum. Indirect immunofluorescence was used to localize chitinase at various developmental stages of five zygomycetous fungi and during abiotrophic mycoparasite interaction with a …
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Involvement of fimbriae in host-mycoparasite recognition
… of the mycoparasite, Piptocephalis virginiana. Polyclonal antiserum (AU) prepared against the fimbrial protein of Ustilago violacea cross-reacted with 60 and 57 kDa M. candelabrum proteins. In addition, AU cross-reacted with 64 kDa proteins from both M. pusilla and P. articulosus. The proteins …
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Function and pharmacology of TRPM3 ion channel
… the 3rd extracellular loop (E3) region, to which polyclonal antiserum (TM3E3) was produced. Extracellular application of TM3E3 inhibited TRPM3 function with a high degree of specificity, having no effect on TRPM2 or example members of other sub-types of mammalian TRP, TRPC5 or TRPV4. The data …
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Molecular analysis of the dry rot fungus Serpula lacrymans
… profile for this organism to be established. A polyclonal antiserum raised against standard mycelium of S. lacrymans FPRL 12C was used as the immunological probe. <br/><br/>The profiles of other isolates of S. lacrymans and different Basidiomycetes were compared with FPRL 12C. Protein and …
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Host-parasite interactions of barley yellow dwarf virus and oats: Physiological and immunological studies
… fluids, and seven MAbs were biotinylated. The polyclonal antiserum resulting from four injections of purified MAV detects stress-related oat antigens. Two greenhouse experiments were conducted to study the induction of stress antigens. Several treatments were applied to uninoculated and MAV …
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Isolation and analysis of a corprinus cinereus DNA fragment showing homology to a fimbrial cDNA from ustilago violacea
… of all fungal classes. In the present study, polyclonal antiserum raised against the fimbrial proteins of U. vio/acea were used in order to identify antigenically related proteins from Coprinus cinereus and Schizophy//um commune. Two polypeptides with molecular masses of 37 and 39 kDa from C. …
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The molecular characterisation of pregnancy-associated plasma protein-A (PAPP-A).
… characterisation of PAPP-A was performed. The polyclonal antiserum (DAKO) directed against PAPP-A has been shown to also interact with proteins other than PAPP-A. These non-specific interactions were abolished by performing Western blotting immunodetection at a high salt concentration (0.6M …
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Transformation of Brassica napus cv. Westar with the beet western yellows virus coat protein gene
… immunosorbent assay (ELISA) using a polyclonal antiserum which reacts with BWYV CP subunits. Preliminary evaluation of the progeny of two promising lines (154802-3 and 1557-7), demonstrated to have the BWYV CP gene integrated into the plant genome, did not reveal significant levels of …
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Anti-idiotype antibodies and phage displayed peptides as antigenic mimics of a Mycoplasma capricolum subsp. capripneumoniae epitope
… were not detectable using Mccp-specific goat antiserum suggesting that the serum did not contain paratopes that could accommodate the surrogate epitopes. In spite of this, the antiserum efficiently inhibited the binding of the Mab to immobilised mycoplasmal lysate in a standardised test for …
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Isolation von Ascosporen des pflanzenpathogenen Pilzes Venturia inaequalis und Charakterisierung von Antikörperinteraktionen zur Entwicklung eines immunogravimetrischen Sensors
… requirements of the sensor. To this end, a mouse polyclonal antiserum was available containing antibodies directed against Venturia spores. Treatment of spores with various enzymes showed that the antigens and the spore-surface had different characteristics. One set of the antibodies in the serum …
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Pharmacological and Molecular Characterisation of P2Y Receptors in Endothelial and Epithelial Cells
… an immunogen to generate and fully characterise polyclonal rabbit antibodies to the P2Y2 receptor. Other aims of this study were to characterise epithelial and endothelial cells for a thorough expression profile of P2Y receptor mRNA transcripts in order to provide a rapid screen for the molecular …
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Developing a diagnostic assay for Pneumocystis jirovecii pneumonia and investigating the interaction of Pneumocystis murina and respiratory syncytial virus during co-infection in mice
… poorly understood. In this study we evaluated a polyclonal antibody (pAb) raised against a putative Pneumocystis biomarker (Kex1), with the outlook of developing a lateral-flow assay (LFA) for rapid point-of care diagnosis of PCP. Kex1 is an antigenically stable serine protease highly conserved …