Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 3 of 3 for “"polybrene"”.
-
Analysis of charged polymer effects on recombinant retrovirus-mediated gene transfer success
Poly-l-lysine and hexadimethrine bromide (polybrene) are cationic polymers which are frequently used to enhance the transduction efficiency of recombinant retroviruses in gene therapy experiments. Conversely, chondroitin sulfate proteoglycan is an anionic polymer endogenously present in retrovirus …
-
Engineered Tracking and Delivery of Mesenchymal Stem Cells (MSCs)
… <i>in vivo</i> in real time. It was found that polybrene, an additive commonly used during transduction, severely inhibited MSC proliferation. A new method was developed for efficient lentiviral transduction using protamine sulfate, and other techniques. MSC engraftment to sites of injury was …
-
IN VIVO IMAGING OF STEM CELL MEDIATED TREATMENT IN A MOUSE MODEL OF SPINAL CORD INJURY
… of carriers such as poly-L-Lysine (PLL), polybrene (PB) and protamine sulphate (PS). PLL and PS were tested at different ratio (Fe/PLL 1:0,03, 1:0,06 and 1:0,09 and Fe/PS 1:0,025 and 1:0,05). Labelled cells were analysed for viability, iron content (Perl’s Staining and spectrophotometer …