Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"polyacrylamide gels"”.
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Functional Microperiodic Polyacrylamide Gels Fabricated by Opal Inversion and Direct-Write Processes
Functional three-dimensional microperiodic polyacrylamide hydrogel structures were created and characterized. Submicron to 50 micron periodicities were explored across the structures, the lower end for optical and filtration potential and the larger for cell growth scaffolds. Two synthesis methods …
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Scattering properties of polyacrylamide gels and their relation to protein electrophoretic migration
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Nuclear Engineering, 1994, and Thesis (B.S.)--Massachusetts Institute of Technology, Dept. of Physics, 1994.
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Ion-affinity electrophoresis in modified polyacrylamide gels and some studies on mechanism of aldolase A action
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Protein Composition of the Outer Membrane of Rhodopseudomonas Sphaeroides: Isolation of the Major Outer Membrane Protein
… sphaeroides was characterized on SDS-polyacrylamide gels. Solubilization of the outer membrane at 75(DEGREES)C as opposed to room temperature solubilization resulted in the dissociation of 75, 72, and 68 kilodalton polypeptide aggregates into 29, 26.5, and 21.5 filodalton polypeptides, …
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High-throughput multiplexed transcriptome and protein measurements in single cells
We present cell gels, a platform for high-throughput multiplexed measurements of transcriptome and proteome in single cells. This method takes advantage of droplet barcoding and hydrogel chemistry to capture mRNAs and proteins in thousands of cells. We report the challenges of acquiring sequencing …
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Studies on the Proteolytic Activation of the Membrane Enzyme, Pyruvate Oxidase
… weight to 56,000 on sodium dodecyl sulfate polyacrylamide gels. However, sedimentation velocity studies combined with quasi-elastic light scattering results show that the proteolysis has no effect on the structure of the native tetramer. Amino-terminal analysis of native and …
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Production, purification and properties of Bacillus thuringiensis neutral protease
… potato starch adsorption. Electrophoresis on SDS-polyacrylamide gels showed a single protein band at the last purification step. The enzyme had a pH optimum around pH 6.5-7.0 and was sensitive to metal chelating agents such as EDTA and O-phenanthroline. The molecular weight of the neutral protease …
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Steroid Stimulated Protein Synthesis in Fish Cell Cultures
… new protein bands as detected by silver staining polyacrylamide gels or fluorography.
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Tanned gelatin: some biophysical and biochemical properties of a new gel exclusion agent and its application to the chromatography of proteins and viruses
… strength of the granules. The dextran and polyacrylamide gels have in general a rigidity equal to that of agar and agarose, but cannot be prepared with such large pore sizes and are therefore limited to the separation of smaller molecules.
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Developmental expression of a 4.5S RNA and variants of U1snRNA in Drosophila melanogaster
… by electrophoresis through high percentage polyacrylamide gels. The Drosophila 4.5S RNA migrated at the same rate as the mouse 4.5S$\sb{\rm I}$ RNA standard. Chemical sequence analysis of the 3$\sp\prime$ end of the Drosophila 4.5S RNA revealed a 60% nucleotide homology with the mouse …
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The Purification and Characterization of The Cytochrome D Containing Terminal Oxidase of Escherichia Coli (Bioenergetics, Membranes, Ultracentrifugation)
… judging from the Coomassie blue staining of SDS-polyacrylamide gels. These gels showed that the cytochrome contained two types of subunits with molecular weights of 57,000 Daltons and 43,000 Daltons from the analysis of a Ferguson plot, or 53,000 Daltons and 28,000 Daltons from the analysis of …
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Polyacrylamide hydrogel friction controlled by time-dependent surface energy due to poroelastic relaxation
… contact area, contact time, and sliding speed of polyacrylamide hydrogel. With a comprehensive understanding of the phenomenon, hydrogels could be designed with targeted friction behavior, and be used to design surfaces for tissue engineering and drug delivery. Polyacrylamide gels were tested to …
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Isozyme patterns of selected isolates of Pholiota in the stirps "Adiposa"
… (laccases and tyrosinases), and peroxidases on polyacrylamide gels. Harvesting of different isolates for protein extraction was carried out at comparable stages of development using dry weight determinations and respiration studies as criteria. Peroxidase and phenoloxidase zyrnograms showed wide …
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Mechanically-induced intercellular remodeling of cardiomyocytes by magnetic micromanipulation
… system consisted of patterned linear strands on polyacrylamide gels and mechanical stimulation using magnetic micromanipulation. Cardiomyocytes were patterned on polyacrylamide gel using 25pm and 50pm microchannels. Mechanical stimulation was induced in sections with high densities of magnetic …
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PURIFICATION OF THE REGULATORY SUBUNIT OF RIBONUCLEOSIDE DIPHOSPHATE REDUCTASE FROM CALF THYMUS
… by the observation of a single band on SDS-polyacrylamide electrophoresis gels. The purification procedure involved the use of conventional techniques under conditions known to induce specific changes in the structural and binding properties of the enzyme. The first half of the purification …
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Fabrication of topographically patterned hydrogel substrates and their influence on cell morphology
… for fabricating topographically patterned hydrogels for use as engineered microenvironments for cells. Polyacrylamide gels were cast from silicon masters by a process that involved ultrasonically vibrating the master during polymerization. Gels were then covalently modified with an even coat of …
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ΧΑΡΑΚΤΗΡΙΣΜΟΣ ΤΗΣ ΓΛΥΚΟΛΙΠΟΠΡΩΤΕΙΝΗΣ (G.L.P.) ΤΟΥ SLIME ΤΡΙΩΝ ΠΡΟΤΥΠΩΝ ΣΤΕΛΕΧΩΝPSEUDOMONAS AERUGINOSA ΚΑΙ ΣΥΓΚΡΙΤΙΚΗ ΜΕΛΕΤΗ ΑΥΤΗΣ ΩΣ ΠΡΟΣ ΤΙΣ ΟΜΟΙΟΤΗΤΕΣ Η ΤΙΣ ΔΙΑΦΟΡΕΣ ΜΕ ΤΟ ΛΙΠΟΠΟΛΥΣΑΚΧΑΡΙΤΗ (L.P.S.) ΤΟΥ ΜΙΚΡΟΟΡΓΑΝΙΣΜΟΥ
… AND PAC 605. ELECTROPHORETIC ANALYSIS ON SDS- POLYACRYLAMIDE GELS AND STRAINING WITH SILVER AND COOMASSIE BLUE SHOWED DIFFERENT PROFILES BETWEEN LPS SLIME AND OUTER MEMBRANES IN ALL THREE STRAINS. COMPARATIVE ANALYSIS OF THE SACCHARIDE MOIETY, BETWEEN LPS AND SLIME OF EACH STRAIN BY HPLC …
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The penetration protease of the cercariae of Schistosoma mansoni
… developed for examining cercarial proteases in polyacrylamide gels containing SDS and copolymerized gelatin substrate. Bands of proteolytic activity could be detected by negative staining. This method was used to show that cercarial secretions contained one major protease with a molecular weight …
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Kidney stone rarity in South Africa's black population : investigation of the biochemical and physico-chemical properties of Tamm Horsfall Mucoprotein as a possible contributory factor
… and verified by electrophoresis on SDS-polyacrylamide gels. MALDI-TOF mass spectroscopy was used to determine the molecular weights of the protein from each group and their respective amino acids were analysed. All four THP groups were subjected to tryptic finger printing digestion and …
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