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Showing 1 to 20 of 40 for “"poly-L-lysine"”.
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Development of mouse morulae after encapsulation in alginate microgels or poly-l-lysine microcapsule
… in either 2% sodium alginate or 0.1% poly-L-Iysine (PLL). In Experiment 1, rate of development of ZPI embryos (n = 150) from morulae to hatched blastocysts was measured after encapsulation in alginate or PLL and as unencapsulated controls. Following encapsulation, developmental stages …
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The Effect of Poly-L-Lysine Concentration, Molecular Weight, and Encapsulation Temperature on Microencapsulated Bovine Spermatozoa
… studies were conducted to evaluate the effect of poly-l-lysine concentration, molecular weight, and encapsulation temperature on the post encapsulation survivability of spermatozoa. Viability of spermatozoa encapsulated at 2012 C using four poly-l-lysine concentrations (.05%, .15%, .25%, and .35%) …
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Investigation of the interaction of GdnHCl with poly-L-lysine, horseradish peroxidase and cytochrome c monitored via FTIR, CD and fluorescence spectroscopies
… interaction of GdnHCl with three model systems: poly-L-lysine (PLL), horseradish peroxidase (HRP) and cytochrome c. Probing the chemically denatured states of proteins by FTIR is a novel technique, which has only recently been reported. To aid in the interpretation of the FTIR results, …
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Conformational and Orientational Dynamics of Semi-Rigid Macromolecules in Shear Flow
… conformational and phase stability of aqueous Poly-L-Lysine solutions in shear flow. Poly-L-lysine exists as an $\alpha$-helix at high pH and a random coil at neutral pH. When the $\alpha$-helix is heated above 27$\sp\circ$C, the macromolecule undergoes a conformational transition to a …
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Engineering semiconductor quantum dots for quantitative imaging of cell motility and invasion
… matrix (ECM) proteins. QDs coated with poly-maleic anhydride (PMA), which had been modified to present alkane, alkene, alkyne, PEG and carboxylic acid, carboxylic acid, and solely PEG, were incubated on poly-l-lysine, collagen, fibronectin, and gelatin coated glass coverslips. Based upon …
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The Engineering and Development of pH-Responsive Biopolymers for Drug Delivery Applications
… the structure of biocompatible pH responsive polymers and their interaction with the biological system in order to design more effective entities for biomedical applications. A systemic approach was used to study the structure and biological interaction of pH responsive biocompatible poly …
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Nanoscale and microscale approaches for engineering the in vitro cellular microenvironment
… patterned by using molding or printing methods. Poly(ethylene glycol)-based molecules and polysaccharides were used to control cell-substrate interactions and to prevent cell adhesion on specific regions of a substrate. To control cell-cell contact, layer-by-layer deposition of ionic biopolymers …
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Aspects of ultrasonic relaxation spectrometry
… to studyproton transfer to the c-amino group of poly-L-lysine as afunction of pH and polypeptide concentration. The relaxation data, obtained by several workers and relating to proton transfer in a range of amino-acids has been analysed using the Debye-Smoluchowski - Eigen equations in an attempt …
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Modular Nanoparticles for Selective Cell Targeting
… for selective cell targeting. Specifically, polyelectrolyte complexes composed of heparin (Hep, a growth factor binding glycosaminoglycan) and poly-L-lysine (PLL, a homopolymeric lysine) were prepared via a pulse sonication method. The Hep/PLL core NPs were further layered with additional …
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Neuro-Silicon Interface of a Hirudo medicinalis Retzius Cell Integrated with Field Effect Transistor
… processing methods currently available at Cal Poly. The types of transistors and cells used, the methods for dissecting and preparing the cells, the electrophysiology methods for validating the experiments, and portions of the design of the junction were based on Fromherz’s 1991 work. Other …
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Scalable production of cellular aggregates for the differentiation of embryonic stem cells into cardiac muscle
… encapsulated in an alginate solution coated with poly-L-lysine using an established drip method and a novel fluorinated oil "floating drop" method. Results from these experiments demonstrate that both methods can be used for encapsulating and growing cells. However aggregation, an important aspect …
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Analysis of charged polymer effects on recombinant retrovirus-mediated gene transfer success
Poly-l-lysine and hexadimethrine bromide (polybrene) are cationic polymers which are frequently used to enhance the transduction efficiency of recombinant retroviruses in gene therapy experiments. Conversely, chondroitin sulfate proteoglycan is an anionic polymer endogenously present in retrovirus …
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2D and 3D patterning of molecules and cells
… Neurons on glass. Cell adhesive synthetic Poly-L-Lysine patterns are built juxtaposed from a non-adhesive silane background and control the distribution and growth of Hippocampal Neurons. Results from large scale patterning and analysis (44000 neurons/sample) show that neural somata align …
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Experimental (spectroscopic) and theoretical studies of rhodanine (2-Thio-4-Oxothiazolidine) and its derivatives
… for SERS analysis is the presence of HC1 and poly(L-lysine) at pH 2.3 (?o = 632.8 nm). The SERS studies also revealed the presence of a strong band at 1566/1575/1572 cm-1 (for rhodanine, 3-aminorhodanine and 3-methylrhodanine, respectively), irrespective of experimental conditions used. The …
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Multi-mode two-dimensional infrared spectroscopy of peptides and proteins
… amide I'-II' spectra of an ideal protein system, poly-L-lysine, provides direct evidence for the structural sensitivity of the amide II' vibration, particularly to the !-helix moiety. This structural sensitivity arises from through bond coupling and structure induced symmetry and orientation of …
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Use Of Cryoprotectants, Prebiotics And Microencapsulation of Bacterial Cells in Improving the Viability of Probiotic Organisms in Freeze-Dried Yoghurt
… technique after freeze-drying. Coating with poly-L-lysine or chitosan further improved the survival of the probiotic organisms by 0.03 and 0.07 log10 cfu/g in yoghurt during 4 weeks of storage at 4ºC. The survival of probiotic organisms in yoghurt stored at 4ºC was highest (8.69 log10 cfu/g) …
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2D IR spectroscopy and computational modeling : application to protein folding and binding
… of NMA, the amide 1'-II' spectroscopy of poly-L-lysine, isotope-edited 2D IR spectroscopy of trpzip2, and transient 2D JR spectroscopy of ubiquitin unfolding after a temperature jump. The emerging paradigm is to interpret 2D IR spectra with the aid of an atomistic, molecular dynamics …
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The activity of pH membrane disruptive pseudopeptides and their subcellular fate in mammalian cells cultured in-vitro
… The pseudopeptides were obtained by the copolymerisation of L-lysine and L-lysine ethyl-ester with various dicarboxylic acid moieties. In this way a linear polyamide comprising of alternating pendant carboxylic acids and pendant hydrophobic moieties was made. At physiological pH (pH 7.4), …
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