Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 242 for “"pluripotent stem cells"”.
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Modeling Alzheimer's Disease in Induced Pluripotent Stem Cells
… follows, we utilized recent advances in induced pluripotent stem cell (iPSC) and genome editing technologies to investigate two separate AD disease mechanisms: tau spreading and Aβ production in familial AD (FAD) PSEN1 mutants. Using TALENs, we generated a transgenic donor iPSC line that harbors …
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Modelling Brugada Syndrome using induced pluripotent stem cells
… to reprogram dermal fibroblasts to induced pluripotent stem (iPS) cells and to differentiate these into cardiomyocytes with the same genetic background provides a novel approach to studying inherited cardiac channelopathies with advantages over existing model systems. Whilst this technique …
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Correction of Cystic Fibrosis-Specific Induced Pluripotent Stem Cells
… to genetically modify the resulting induced pluripotent stem cells (iPSCs) have provided an alternative to conventional gene therapies thus far attempted for CF. Here we describe the <em>in vitro</em> generation of iPSCs starting from fibroblast cells obtained from a CF patient. These cells, …
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Derivation of lymphocytes from human induced pluripotent stem cells
Human pluripotent stem cells have the potential to produce essentially unlimited numbers of mature and functional blood lineage populations to study human hematopoiesis. Particularly, human induced pluripotent stem cells (hiPSCs) have the advantage to provide a source of autologous transplantable …
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Modeling Monogenic Diabetes Mody3 Using Human Pluripotent Stem Cells
… not recapitulate the disease. Here, we use human pluripotent stem cells (ESCs) and CRISPR-CAS9 (Clustered Regularly Interspaced Short Palindromic Repeat)/Cas9) nucleases to understand the most common form of monogenic diabetes, Maturity Onset Diabetes of the Young 3 (MODY3) caused by heterozygous …
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HIGHLY SPECIFIC FLUORESCENT PROBE FOR MOUSE PLURIPOTENT STEM CELLS
… PCR data, showed that CDy9 bright populations of cells were positive for pluripotent genes. It was also found that CDy9 binds to mESC stably and showed minimal toxicity to mESC. CDy9 was also applied to mouse abdominal fats and was shown to isolate potential pluripotent stem cells. Thus, CDy9 can …
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Using human induced pluripotent stem cells to model cystinosis
… accumulation of cystine within lysosomes in most cells of the body, however the kidney is the main organ affected. For poorly understood reasons, renal proximal tubule (PT) cells lose their functionality and degenerate in cystinosis, forming a characteristic “swanneck” lesion that leads to kidney …
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Modeling Complex Neurological Disorders With Human Induced Pluripotent Stem Cells
… due to a lack of accurate models. Human-induced pluripotent stem cells (HiPSCs), which can be differentiated into any cell type and capture the genetic variation across humans, may serve as a powerful model to elucidate the mechanisms behind such disorders. In this thesis we have explored …
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Extracellular matrix based substrates for propagation of human pluripotent stem cells
In human pluripotent stem cell (hPSC) research and applications, the need for a culture system devoid of non-human components is crucial. Such a system should exhibit characteristics observed in conventional culture systems that have used mouse embryonic fibroblast feeders for hPSC self renewal …
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Efficient In Vitro Development of Photoreceptors from Human Pluripotent Stem Cells
… retina relies on the insertion of new, healthy cells. Embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are two possible sources of photoreceptors to restore vision. Previous data shows that human ES cells and iPS cells can be differentiated into photoreceptors and transplanted …
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Investigating Induced Pluripotent Stem Cells for Tissue Engineering and Hepatotoxicity Applications
Induced pluripotent stem cells (iPSCs) can be differentiated into multiple cell types in the body while maintaining proliferative capabilities. The generation of human iPSC-derived hepatocytes (iPSC-Heps) has resulted in a new source for hepatic cells. The current available options for human …
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Modeling Cancer Using Li-Fraumeni Syndrome Patient-Derived Induced Pluripotent Stem Cells
… LFS patients' fibroblasts to induced pluripotent stem cells (iPSCs), and further differentiate these iPSCs into mesenchymal stem cells (MSCs) then to osteoblasts (OBs), the cells from which osteosarcomas originate. Interestingly, LFS iPSC-derived osteoblasts recapitulated the …
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DNA Repair Deficiency in Huntington's Disease Fibroblasts and Induced Pluripotent Stem Cells
… disease-causing TNR expansions occur in somatic cells remains elusive. Interestingly, HD has often been referred to a ‘DNA repair disease’, even though DNA repair dysfunction <em>in situ</em> has not been identified. We hypothesized that presence of the mhtt protein affects the expression of DNA …
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Development of collagen-based scaffolds for differentiation of induced pluripotent stem cells
… engineering. A collagen hydrogel can provide cells with a porous and soft environment to proliferate and differentiate. However, lacking mechanical stiffness and shrinkage resistance made it a challenge to sustain shape and size during a long stem cell differentiation process. In addition, a …
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Production of hepatocytes from human pluripotent stem cells for cell-based therapy
Human pluripotent stem cells (hPSCs) can self-renew indefinitely in vitro while maintaining the capacity to differentiate into diverse cell types, including hepatocytes. Thus, hPSCs allow for large-scale production of hepatocytes necessary for cell-based therapy. Importantly, cell-based therapy …
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Investigation of genetic PIK3CA activation in genome-edited human pluripotent stem cells
… by establishing isogenic series of human induced pluripotent stem cells (iPSCs) with endogenously expressed, activating PIK3CA mutations. This involved the optimisation of a CRISPR/Cas9 protocol for efficient knockin of different PIK3CA variants into human iPSCs. An isogenic iPSC series was …
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The use of human pluripotent stem cells to model HNF1B-associated diabetes
… nuclear factor 1B (HNF1B), result in multisystem disease including diabetes due to beta-cell dysfunction and pancreatic hypoplasia. However, the mechanisms that underlie development of diabetes in HNF1B mutation carriers are still not fully understood due to lack of an appropriate model …
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MODELING HEART DISEASE OF PATIENTS WITH MUSCULAR DYSTROPHY USING INDUCED PLURIPOTENT STEM CELLS
… DMD patient cardiomyocytes derived from induced pluripotent stem cells (iPSC). By converting adult somatic cells to iPSCs and further differentiate into cardiac lineage, a vast number of cardiomyocytes can be manufactured to explore disease etiology and conduct drug screening.
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