Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 443 for “"pluripotent"”.
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Modeling Alzheimer's Disease in Induced Pluripotent Stem Cells
… follows, we utilized recent advances in induced pluripotent stem cell (iPSC) and genome editing technologies to investigate two separate AD disease mechanisms: tau spreading and Aβ production in familial AD (FAD) PSEN1 mutants. Using TALENs, we generated a transgenic donor iPSC line that harbors …
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Modelling Brugada Syndrome using induced pluripotent stem cells
… to reprogram dermal fibroblasts to induced pluripotent stem (iPS) cells and to differentiate these into cardiomyocytes with the same genetic background provides a novel approach to studying inherited cardiac channelopathies with advantages over existing model systems. Whilst this technique …
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CHARACTERISING THE REPROGRAMMING DYNAMICS BETWEEN HUMAN PLURIPOTENT STATES
Human pluripotent stem cells (hPSCs) exist in multiple states of pluripotency, broadly categorised as naïve and primed states. These provide an important model to investigate the earliest stages of human embryonic development. Naïve cells can be obtained through primed-to-naïve reprogramming; …
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Correction of Cystic Fibrosis-Specific Induced Pluripotent Stem Cells
… to genetically modify the resulting induced pluripotent stem cells (iPSCs) have provided an alternative to conventional gene therapies thus far attempted for CF. Here we describe the <em>in vitro</em> generation of iPSCs starting from fibroblast cells obtained from a CF patient. These cells, …
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Derivation of lymphocytes from human induced pluripotent stem cells
Human pluripotent stem cells have the potential to produce essentially unlimited numbers of mature and functional blood lineage populations to study human hematopoiesis. Particularly, human induced pluripotent stem cells (hiPSCs) have the advantage to provide a source of autologous transplantable …
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Modeling Monogenic Diabetes Mody3 Using Human Pluripotent Stem Cells
… not recapitulate the disease. Here, we use human pluripotent stem cells (ESCs) and CRISPR-CAS9 (Clustered Regularly Interspaced Short Palindromic Repeat)/Cas9) nucleases to understand the most common form of monogenic diabetes, Maturity Onset Diabetes of the Young 3 (MODY3) caused by heterozygous …
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HIGHLY SPECIFIC FLUORESCENT PROBE FOR MOUSE PLURIPOTENT STEM CELLS
… bright populations of cells were positive for pluripotent genes. It was also found that CDy9 binds to mESC stably and showed minimal toxicity to mESC. CDy9 was also applied to mouse abdominal fats and was shown to isolate potential pluripotent stem cells. Thus, CDy9 can selectively stain mESC …
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microRNA expression profile of undifferentiated and differentiating pluripotent cells
… wurden mit einer zusätzlichen pluripotenten Zelllinie, EGCs (Embryonic Germ Cells-embryonale Keimbahn-Stammzellen), erweitert. Bezüglich der Exprimierung von Mitgliedern des miR-290 und miR-17-92 Clusters zeigten die EGCs während der Differenzierung große Ähnlichkeiten mit …
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Using human induced pluripotent stem cells to model cystinosis
… model of cystinosis, our lab has generated human pluripotent stem cells from a cystinosis patient (CTNS-iPS). The aim of this thesis is to characterise the cellular phenotype of CTNS-iPS cells and to develop protocols to differentiate these cells into cystinotic PT cells for further study. …
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Transcriptional Regulation by DAX-1 in Pluripotent and Differentiated Cells
… been shown to be an important contributor to the pluripotent state. Potential downstream targets of Dax-1 were previously identified based on significant changes in expression when Dax-1 expression was down regulated. Two methods, siRNA and CRISPR-Cas9, were used to decrease Dax-1 expression in …
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Modeling Complex Neurological Disorders With Human Induced Pluripotent Stem Cells
… due to a lack of accurate models. Human-induced pluripotent stem cells (HiPSCs), which can be differentiated into any cell type and capture the genetic variation across humans, may serve as a powerful model to elucidate the mechanisms behind such disorders. In this thesis we have explored …
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Engineering pluripotent stem cell heterogeneity and its effects on differentiation
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-05-01
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Extracellular matrix based substrates for propagation of human pluripotent stem cells
In human pluripotent stem cell (hPSC) research and applications, the need for a culture system devoid of non-human components is crucial. Such a system should exhibit characteristics observed in conventional culture systems that have used mouse embryonic fibroblast feeders for hPSC self renewal …
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Efficient In Vitro Development of Photoreceptors from Human Pluripotent Stem Cells
… cells. Embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are two possible sources of photoreceptors to restore vision. Previous data shows that human ES cells and iPS cells can be differentiated into photoreceptors and transplanted into the eye to restore some vision. However, …
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Defining the essential regulators of naive human pluripotent stem cell reprogramming
Human pluripotent stem cells (PSCs) exist in two distinct states – naïve and primed, which are interconvertible. Naïve PSCs uniquely harbour several desirable properties that primed PSCs do not, including their ability to model human pre-implantation biology and developmentally-regulated epigenetic …
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The role of mechanotransduction in signalling and pluripotent stem cell fate
… evidence suggests that the regulation of pluripotent stem cell fate is strongly influenced by external mechanical factors, the mechanisms of interplay between soluble factor signalling and mechanical cues remain unclear. Further understanding of how mechanical factors of the extracellular …
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Investigating Induced Pluripotent Stem Cells for Tissue Engineering and Hepatotoxicity Applications
Induced pluripotent stem cells (iPSCs) can be differentiated into multiple cell types in the body while maintaining proliferative capabilities. The generation of human iPSC-derived hepatocytes (iPSC-Heps) has resulted in a new source for hepatic cells. The current available options for human …
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Extracellular matrix- and pluripotent stem cell-based tissue engineering of the kidney
… endothelial cells differentiated from induced pluripotent stem cells. This approach required the development of a perfusion bioreactor and control software, which enabled the de- and recellularization of the kidneys as well as the subsequent in vitro cultivation. Decellularization is the …
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