Global ETD Search
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Showing 1 to 15 of 15 for “"plastome"”.
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An Investigation into Transformation and RNA Editing in the Lolium Plastome
Ireland’s agricultural land area comprises of 91% grassland, which predominantly consists of perennial ryegrass (Lolium perenne L.). Owing to the predicted climate change, grasslands in the east of Ireland will be severely affected over the next hundred years. Due to this prediction, strategies to …
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The complete plastid genome sequence of Passiflora cincinnata: genome rearrangements, massive plastid gene losses and implications to genome-plastome incompatibility
The plastid genome (plastome) organization, gene content and order is well conserved in most angiosperms, but there are some exceptions. The Passiflora genus is one of those exceptions, because there are evidences of some unusual plastid gene losses to species of this genus. However, none plastome …
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Improved plastid transformation in Marchantia polymorpha
… plants in which they reside, which is termed the plastome (plastid genome). This genome encodes many of the proteins required for photosynthesis, as well as bacteria-like transcriptional and translational machinery. Biotechnological techniques for making precise modifications to the plastome have …
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Plastomes of the subfamily Cactoideae (Cactaceae): genomic, structural and evolutionary aspects
… unusual features have been identified in the plastomes of the family. Thus, to expand the information concerning plastid evolution in Cactaceae, this study aimed at sequencing, assembling and analyzing the plastomes of M. glaucescens, L. cruciforme, and S. truncata. In addition, to provide …
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Chloroplast DNA phylogenetics of the North American chestnuts and chinquapins (Castanea Mill., Fagaceae)
… using chloroplast DNA sequence data. Six plastome loci were PCR-amplified and sequenced in 77 accessions representing the three currently recognized North American Castanea species. Diagnostic morphological character states and a unique haplotype were shared among C. pumila and a plant …
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Improving the Phylogenetic Understanding of the Genus Juniperus
… increased resolution and support by generating a plastome-scale phylogeny for 28 <em>Juniperus</em> species, revealing previously unresolved relationships at both deep and shallow nodes. One-third of the recognized species of <em>Juniperus</em> are included, representing each of the major clades …
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Understanding angiosperm genome interactions and evolution: insights from sacred lotus (Nelumbo nucifera) and the carrot family (Apiaceae)
… of transposable elements. The ‘China Antique’ plastome retains the ancestral gene synteny of Amborella and has no evidence of IGT. ‘China Antique’ has more smaller repeats in its mitochondrial genomes than reported for other angiosperms, but does not contain any large repeats, and its nuclear …
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Markerfreie transplastome Tabakpflanzen
… can never become stably integrated into the plastome and gets simply lost once the antibiotic selection pressure is removed. Phenotype-assisted selection of plastid transformants in combination with a transient selection marker is a new efficient system for the rapid production of marker-free …
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Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.)
… PCR analysis, but an integration of DNA into the plastome could not be verified so far. Efficient regeneration systems and methods of DNA transfer were established for rapeseed and sugarbeet and straightened the way for successful plastid transformation in either species.
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Tulipa: the taxonomy and evolutionary history of the genus and its impact on conservation priorities in Central Asia
… three fieldwork seasons, to generate both a plastome based and 35S rDNA phylogeny. These phylogenies allowed us to make a number of taxonomic decisions with respect to the synonymization and reinstatement of a number of species. We also reorganised the higher-level taxonomic groups of this …
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Analysis of cis-acting expression determinants of the tobacco psbA 5’UTR in vivo
… and uidA had been correctly integrated into the plastome at the insertion site, and transcribed in discrete sizes. Quantitative assays were also done to determine the proportion of intact transplastome, the uidA mRNA level, Gus activity, and uidA translation efficiency. The main results are the …
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Plastid Genome Evolution in the Parasitic Genera Cuscuta (Convolvulaceae) and Krameria (Krameriaceae)
… This is particularly true for plastid genomes (plastomes) given that chloroplasts are the primary site of photosynthesis in the cell and that plastomes encode key portions of the photosynthetic apparatus.In only a handful of embryophyte lineages, however, a heterotrophic lifestyle has evolved …
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Functional analysis of plastid-encoded genes
… states, and to distinguish between plastome-located wild-type segments in transplastomic material and promiscuous DNA, a new approach was developed. Customary Southern and PCR techniques are not sensitive enough or not discriminating the latter alternatives, respectively. …
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Deletion plastidärer ribosomaler Proteine in Nicotiana tabacum im Kontext reduktiver Genomevolutionund Entwicklung einer Hochdurchsatzplattform zur Analysevon miRNAs in Chlamydomonas reinhardtii
Im Rahmen des ersten Teils der vorliegenden Doktorarbeit konnten zwei nicht-essentielle (rps15, rpl36) und fünf essentielle (rps3, rps16, rpl22, rpl23, rpl32) im Plastom von Nicotiana tabacum kodierte Proteine des plastidären Ribosoms bezüglich ihrer Essentialität charakterisiert werden. Diese Gene …
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Molecular Systematics of <i>Isoëtes</i> (Isoëtaceae) in Eastern North America
<p><em>Isoëtes</em> (Isoëtaceae, Isoetales, Lycopodiophyta) is a cosmopolitan genus of aquatic lycophytes, occurring on every continent except Antarctica. Of approximately 200 total taxa, about half are in a clade of species mostly occurring in North and South America. Eastern North America …