Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"plasmid vector"”.
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Technical evaluation of a Real-time polymerase chain reaction (PCR) assay for the detection of Bartonella spp for diagnostic purposes
… human samples. For this study, we constructed a plasmid vector containing an insert of 83bp, derived from the Bartonella nuoG gene. In this non-clinical, laboratory evaluation, we used one laboratory sample to amplify the nuoG bacterial DNA fragment and cloned it into a plasmid vector. Using this …
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Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut
… products or after cloning the products into the plasmid vector pBluescript (SK). The results obtained from the 16S rDNA sequence homologies strongly suggests that the newly identified bacteria should be assigned to three major bacterial genera, namely, Ruminococcus, Buryrivibrio and Bacteroides. …
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Iron Acquisition in <em>Rhodococcus erythropolis</em> Strain IGTS8: Characterization of a Mutant Strain that Over Produces Siderophore.
… for over production of siderophore into a plasmid vector. The DNA sequence of this region was determined and analyzed for the presence of similar genes encoding transport proteins.</p>
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Recombinant Expression In Pichia pastoris System Of The Potassium Channel Inhibitor Peptide, Ts6.
… Ts6 extracellularly in substantial amount. The plasmid vector pPICZαA-Ts6 was constructed, where the N-terminal was his-tagged and an enterokinase cleavage site was inserted. The X-33 Pichia clones were cultured in increasing concentration of ZeocinTM. After methanol induction, protein …
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Developing a vector for expressing serratiopeptidase in Lactococcus lactis
… circumvent this issue, we proposed developing a plasmid vector that, when transformed into the probiotic Lactococcus lactis, would allow for bioactive serratiopeptidase production within the gut; thus, increasing bioavailability. The vector designed was delivered from the synthesis company in two …
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Characterization and nucleotide sequence of apple mosaic virus coat protein gene
… RNA3 and cloned into a pBluescript T-vector. The inserts of four clones from the 3$\sp\prime$-end of RNA3 were sequenced and the 3$\sp\prime$-primer was designed to synthesize cDNA of RNA3. The DNA was cloned into the plasmid vector pUC118. A set of overlapping cDNA clones was used to …
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Identification and molecular characterisation of a putative gene for cell detaching factor from Trichomonas vaginalis.
… from Tv genomic DNA by PCR and cloned into the plasmid vector pCR2.1-TOPO. The ORF was transcribed in vitro using T7 RNA polymerase and translated in rabbit reticulocyte lysate in the presence of 35S-methionine. Several high molecular weight proteins (>200, 165, 150, 130, 118, 80, 47 kDa) were …
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Success and limitations of gene therapy with growth factors to enhance cutaneous wound healing
… the injection of a non growth factor expressing plasmid vector. Conversely, however, it was also found that both the repeated injections and the high dose of DNA required to achieve this effect had a detrimental effect on healing. To address this problem, several alternative means of improving …
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Mapping the Major Epitope(s) of the Glycoprotein of Ebolavirus with QB Phage Display System
… its analysis. To prepare the recombinant plasmids, separately, the gene segments of the glycoprotein determinants were designed with overlapping fragments, fused with the A1 gene and amplified by PCR. The obtained gene fragments were separately purified, digested and ligated with a …
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Demonstration of new subtypes of adenovirus 7 in South Africa, and probing oesophageal carcinoma cell lines for evidence of adenovirus or of other oncogenic viruses
… viral DNA. One of these, an Ad7 recombinant plasmid probe, was developed by cloning DNA from the transforming region of the Ad7cl strain into the plasmid vector pUC19. Cellular DNA, extracted from the 3 tumor lines HCU18, HCU33 and HCU39, was tested by means of both DNA dot hybridization and …
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Evolution of a gene for pathogenicity: endo-pectate lyase
… for subcloning and further study. I used the plasmid vector pBR322 to construct a clone bank of Eca strain SRB; of the 1700 clones screened, five were pectolytic. Two of the Eca pectolytic. clones had homology to the Ecc endo-pectate lyase gene; upon examination, they proved to contain the …
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Characterisation of two aphid picorna-like viruses
… synthesized from RhPVOFS RNA and cloned into the plasmid vector, pBR322. This clone was used for the detettion of virus in aphids. ALPV and RhPV are serologically unrelated. ALPV is serologically distantly related to two insect picornaviruses, cricket paralysis virus (CrPV) and Drosophila C virus. …
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The Immunology of Adeno-Associated Virus Serotype 2
… 2 (AAV-2) has been developed as a gene therapy vector. Antibody and cell-mediated immune responses to AAV-2 or AAV-2 transfected cells may confound the therapeutic use of such vectors in the clinic. In this study, cell-mediated and humoral immune responses to AAV-2 were characterised from a …
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Construction of bovine adenovirus type 3 E1 and E3, substitution plasmids and the sequencing analysis of DNA pol and pTP /
… a suitable candidate for the construction of vectors. The use of adenoviral vectors in gene therapy, vaccination, and as a general vector system for expressing foreign genes have been documented for some time. In this study, the objective was to rescue a BAV3 E1 or E3 recombinant vector …
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Development of a cloning system for gene expression in Pasteurella multocida
… of strain P1059 was constructed using the plasmid vector pUC-19 and screened with antisera against live strain P1059; 12 out of 4,100 clones were recognized. The inserts of the plasmids from these clones ranged from 0.48- to 6.S-kilobases (kb) in length. Five of the 12 clones expressed …
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Efficacy of a Magnetic Drug Delivery System and Development of an Orthotopic Lung Tumor Imaging Model
… A549 cells were genetically modified with plasmid and lentiviral vector constructs to stably express hNIS. Modified tumor cells were then implanted into nude mice to develop two tumor models: the subcutaneous xenograft model and a xenograft orthotopic tumor model. Tumor progression was …
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Engineering therapeutic messenger RNA for triple-negative breast cancer
… of this strategy was limited by the use of viral vectors. Messenger RNA (mRNA) technology has been cemented as a safe and effective therapeutic modality, as evidenced by its success in SARS CoV-2 vaccination. Its potential to revolutionise cancer therapy is now becoming increasingly evident. We …
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Molecular Genetic Analysis of Tumor Cells and Developmenet of RNA Interferance Therpy in Neurofibromatosis Type1(NF1)
… of H-Ras has been tried using a episomal plasmid vector, pREP4, and 3 effective RNAi constructs have been identified. A further functional analysis of them may lead to not only a better understanding the mechanisms of tumorigenesis and malignant degeneration of NF1 but also effective …
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Developmental genetic studies on Thiobacillus ferrooxidans
… Conjugation was attempted as a means for plasmid DNA transfer to T. ferrooxidans. Recombinant T. ferrooxidans plasmids, pDER401 and pDER405, were shown to code for mobilization and replication functions in Escherichia coli and Thiobacillus novellus strains. The plasmids were mobilizable at …
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