Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 134 for “"plasmid DNA"”.
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Cloning and sequence analysis of plasmid DNA from Helicobactor pylori
… gastric ulcers. Many bacterial species contain plasmid DNA which encodes virulence factors, including antibiotic resistance, adherence factors, degradative enzymes, and toxins. The majority of H. pylori strains contain plasmid DNA, although the size varies from strain to strain. No phenotype has …
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Purification of plasmid DNA for use in human gene therapy
… the focus of this research in manufacturing plasmid DNA for use In human gene therapy. First, the processing of E.coli bacterial cells to effect the separation of therapeutic plasmid DNA from cellular debris and adventitious material. Second, the affinity purification of the plasmid DNA in a …
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The antitumor activity of tumor-targeted RNA replicase-based plasmid DNA
… we propose to deliver a RNA replicase-based plasmid DNA, hypothesizing that the dsRNA generated by the replicase-based plasmid in tumor cells will inhibit tumor growth. We evaluated the anti-tumor activity of a plasmid (pSIN-beta) that encodes the sindbis RNA replicase genes in mice with …
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Development of plasmid dna formulations for application as microspheric dna vaccines
The advent of DNA vaccines has heralded a new technology allowing the design and elicitation of immune responses more adequate for a wider range of pathogens. The formulation of these vaccines into the desired dosage forms extends their capability in terms of stability, routes of administration and …
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Development of new tools for the production of plasmid DNA biopharmaceuticals
DNA vaccines and gene therapies that use plasmid DNA (pDNA) as a vector have gained attention in recent years for their good safety profile, ease of manufacturing, and potential to treat a host of diseases. With this interest comes increased demand for high-yield manufacturing processes. Overall, …
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Cationic liposome-mediated transfection of mammary epithelial cells with AAV-based plasmid DNA
… delivery of adeno-associated virus (AAV) based plasmid DNA has potential for efficient and safe delivery of DNA into a number of different cell types. The objective of this thesis was to develop techniques for transfecting adult somatic mammary epithelial cells with the aim of producing …
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Cationic Polymers and Polymeric Micelles as Plasmid DNA and CRISPR-Cas9 Ribonucleoprotein Delivery Vehicles
… needed. Synthetic polymers have been explored as plasmid DNA delivery vehicles for decades owing to their low production cost, chemical flexibility, low immunotoxicity, and the ability to encapsulate biomacromolecules. However, the precise control of polymeric vehicle properties by …
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Investigation of the immunostimulatory activity and vaccine potential of lipid encapsulated plasmid DNA and oligodeoxynucleoties
DNA vaccines offer unique promise as a means of generating immunity against infectious and malignant disease. Unfortunately a number of obstacles, including rapid degradation of naked plasmid DNA (pDNA), poor cellular uptake by antigen presenting cells (APCs) and subsequent low levels of gene …
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Quantitation of 8-oxoG and strand breaks in pUC19 plasmid DNA after exposure to oxygen radical generating systems
Thesis (M.S.)--Massachusetts Institute of Technology, Division of Toxicology, 1995.
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The Development and Assessment of Immune Responses in Plasmid DNA, VSV-Vectored, and Virus-Like Particle HIV Vaccinations in Murine Models
… vectors: VSV, virus-like particle (VLP), and DNA; 2) immunogens: transmitted founder strain A74 N425 wild type and N425K mutant; and 3) conformational changes: vaccines with or without CD4 mimetic interaction, to identify the best vaccine candidate. We also investigated the mechanisms by which …
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Plasmid deoxyribonucleic acid of strains of the saccharolytic intestinal Bacteroides
… intestinal Bacteroides was examined for plasmid deoxyribonucleic acid (DNA). These strains were previously characterized for standard phenotypic properties (variety of carbohydrates fermented, metabolic end products, and complex carbohydrate hydrolysis). Additional phenotypic …
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The design and analysis of sugar-based carrier systems for the protection of nucleic acids
… carrier systems for the protection of naked plasmid DNA formulations generated by spray drying. Trehalose exists in multiple crystalline anhydrous and hydrated forms and is thought to exist in two different amorphous forms. Reported thermal transitions of trehalose dihydrate vary with …
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Entwicklung eines "DNA-Delivery"-Systems auf der Basis von Virulenz-attenuierten Listerien
… geeignete Vektoren für den Transport von Vakzine-DNA in das Zytosol von Antigen-präsentierenden Zellen ("DNA delivery"). In dieser Arbeit wurde dazu das intrazelluläre Bakterium Listeria monocytogenes verwendet, welches sich im Zytosol von Zellen vermehrt und fortbewegt. Ausgestattet mit einer …
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Free radical formation and DNA damage in the cytotoxicity of alkylaminoanthraquinone antitumour agents.
… dependent reductase mediated redox cycling in DNA strand breakage and cytotoxicity by doxorubicin (DOX), mitozantrone (MIT), CI941 and a series of alkylaminoanthraquinones (AQ) based on MIT has been investigated in MCF-7 human breast cancer cells. lAQ, 1,8AQ and DOX were found to redox cycle in …
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Cellular Responses in ESCHERICHIA COLI to Lethal and Sublethal Doses of Ozone
… <em>E. coli</em> K-12 transformed with pACYC184 plasmid DNA resulted in protein and nucleic acid leakage and production of thiobarbituric acid reactive substances, without affecting cell viability. The intracellular components, protein and plasmid DNA, remained intact. The enzymes malate …
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A Preclinical Model of Ionizing Radiation and TNF-alpha Gene Therapy for Cancer
… study sought to determine if pretreatment with plasmid DNA expressing Tumor Necrosis Factor-α (TNF-α) could enhance the effect of ionizing radiation in an aggressive, rapidly growing glioma tumor (C6) model. Plasmid DNA is an attractive vector system for delivery of foreign genes into eukaryotic …
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Einfluß von Wachstumsfaktoren und Wachstumsfaktorkombinationen auf Angio- und Arteriogenese am ischämischen Kaninchenmyokard : eine histologische Studie
… war, den Einfluss von VEGF-, FGF-2- sowie GM-CSF-Plasmid-DNA auf Angiogenese und Arteriogenese im ischämischen Kaninchenmyokard zu untersuchen. Desweiteren war von besonderem Interesse, ob und bei welcher DNA-Konzentration VEGF und FGF-2 synergistisch wirken könnten. Methoden: Eine Woche nach …
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Modification of a DNA Vaccine for Oral Administration in Fish for Aquaculture by Using Non-Microbial Nanoparticles
Utilization of DNA vaccines in aquaculture has been gaining interest and recent efforts have been focused on methods of delivering DNA vaccines to fish. In the present study, a methodology was sought that could protect DNA vaccines such that they could be orally administered. The main objective of …
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