Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"plasma membrane integrity"”.
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Development of imaging techniques to assess tumour cell death
… enzyme fumarase to produce malate. With loss of plasma membrane integrity in dying cells fumarate can rapidly gain access to the enzyme. By measuring increased malate production in treated tumours after fumarate injection, cell necrosis can be assessed. It could be established that ²H MRS and …
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The role of spectrin in Drosophila photoreceptor development.
… a cytoskeletal protein that interacts with the plasma membrane, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In Drosophila, spectrin is composed of 3 subunits: α-spectrin, β-spectrin, and βH-spectrin. The interaction …
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Function of abscisic acid in maintenance of maize primary root growth under water deficit
… in ROS levels preceded and caused loss of plasma membrane integrity in vp14 roots under water deficits. The results provide conclusive evidence that the maintenance of elongation in the maize primary root requires the accumulation of ABA both to restrict ethylene synthesis and to prevent …
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Relationships between in vivo and in vitro heterospermic ranking, embryo development, and sperm characteristics of Holstein and Jersey bulls
… analysis of DNA fragmentation, acrosome integrity, plasma membrane integrity and disorder, and intracellular Ca2+), or standardized to equal numbers of motile sperm and recombined 1:1 resulting in the 9 H/J pairings for heterospermic IVF (H-VITRO-2). A competitive index (CI) was …
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AVALIAÇÃO ANDROLÓGICA E ULTRASSONOGRAFIA DOPPLER EM GARANHÕES QUARTO DE MILHA
… were evaluated for vigor, volume, concentration, integrity, plasma membrane functionality and motility. Data were analyzed using JMP® 10.0.0 (Demo) and Microsoft Excel 2016 software. The mean values and coefficient of variation were plotted and a correlation analysis of spearman's was performed. …
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Factors causing anterior acrosomal swelling on motile bovine sperm
… folds and/or projections. Outer acrosomal and plasma membrane integrity was retained. Anterior acrosomal swelling (AS) was first observed after neat-semen had been slowly cooled to 4°C and stored for 1 day. With subsequent dilution (25 X 10⁶ sperm/ml) and incubation of sperm in a Tris fructose …
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Nanosecond Pulsed Electric Field Effects on Cell Cycle and Apoptosis
… phenotype characterized by maintenance of membrane integrity, cell shrinkage, phosphatidylserine (PS) externalization at the plasma membrane, caspase protease activation, DNA fragmentation, release of cytochrome c from the mitochondrion, and membrane blebbing. An important regulatory …
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Evaluation of Stallion Frozen-Thawed Semen Using Conventional and Flow Cytometric Assays
… activity using Rhodamine 123 (R123), plasma membrane integrity using propidium iodide (PI) and ethidium monoazide (EMA), and chromatin structure using the sperm chromatin structure assay (SCSA). Data was recorded as percentages for all but the SCSA for both experiment one and two. The …
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Proteomic analysis of cellular models of neurodegeneration and mitochondrial dysfunction
… 5 mM) and time-points (0 to 48 hours) of MPTP on plasma membrane integrity, cellular metabolic activity, cellular ATP concentration, mitochondrial potential, cytochrome c release and a variety of caspase activities were investigated. From this study, sub-cytotoxic and cytotoxic concentrations were …
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Evaluierung durchflusszytometrischer Verfahren zur Beurteilung der Qualität von kryokonserviertem Hengstsperma
… of cryopreserved sperm from stallions. The plasma membrane integrity, the mitochondrial membrane potential, the acrosomal status and the integrity of the chromatin structure in cryopreserved sperm from stallions were assessed. For this purpose various fluorescent stainings were carried out …