Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 121 for “"plant cells"”.
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Cell-walls of growing plant cells
The plant primary cell wall is a three-dimensional interwoven network of cellulose microfibrils, cross-linked by xyloglucan and dispersed in a pectin matrix. It has been suggested that in the wall of growing plant cells, xyloglucan is bound to the rigid cellulose microfibrils by hydrogen bonds and …
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The regulation of division of higher plant cells
… of endogenous IAA (approximately 500-750 ng 10 8 cells) is vital prior to each mitosis. In this present study a more effective induction technique has been employed. The cells were found to rapidly become nitrate and phosphate limited in batch culture. The addition of these nutrients after each …
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Metabolism of Two Model Xenobiotics in Cultured Plant Cells
Plant cells in culture were found to oxygenate two model xenobiotics. Para-chlorophenyl methyl sulfide was S-oxidized by carrot, cotton, tobacco, alfalfa, and blacknightshade cell cultures. The rates of S-oxygenation by cultured plant cells were comparable to the rates in animal tissue …
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Effects of sublethal and lethal temperatures on plant cells
… responses of monocotyledonous and dicotyledonous plants to sublethal and lethal temperatures. The study had four objectives: I. to compare temperatures that develop in stems and leaves of plants under flame, II. to determine the effect of heat on conduction in the phloem (of stems), III. to …
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Studies on the Metabolism of Plant Cells in Tissue Culture
… tissue culture system derived from pith cells of Nicotiana tabacum L. var Wisconsin 38 was established. This system was used to study methods of purifying nucleic acids, phosphate ester and nucleic acid metabolism in cells subjected to nutritional shifts, and problems relating to the …
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DRUG TOXICITY STUDIES BASED ON VIRE2-GFP MOBILITY INSIDE PLANT CELLS
… in real time within Nicotiana benthamiana leaf cells. By monitoring the mobility of VirE2-GFP protein, we can detect toxicity of the difference between control samples and samples subjected to drugs. A total of 9 drugs which consists of both withdrawn and current drugs were tested in this study. …
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Production of recombinant human serum albumin in transgenic plants and plant cells
… The initial attempt was to generate a novel plant codon optimised semi-synthetic HSA gene by PCR amplification. The strategy was to eliminate the rare codons that are used at low frequencies in the plant host system resulting in an optimized codon usage pattern for high-level expression of …
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Biochemical characterization of nitrate transport at the plasma membrane of plant cells using isolated vesicles
A modified method for the production and purification of $\sp{36}$ClO$\sb3\sp{-}$ in high yield is described. This procedure, involving the elctrolytic oxidation of $\sp{36}$Cl in a cell with simple electrode design and purification of the electrolysis products ($\sp{36}$Cl, …
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Localization of Red Clover Necrotic Mosaic Virus Polymerase Proteins at the Endoplasmic Reticulum of Living Plant Cells
… localization of the RCNMV replicase proteins in plants using green fluorescent protein (GFP) fusions observed in live cells using confocal microscopy. The GFP:p27 and GFP:p88 fusions were expressed in N. benthamiana epidermal cells and protoplasts. GFP:p27 consistently localized to the …
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Yeast and plant cells as biofactories for recombinant proteins : evaluation of novel expression and downstream processing strategies
… from crude yeast fermentation broth or plant cell extract, with special emphasis on scalability and cost-effectiveness. Expanded bed IMAC was successfully introduced as a novel method for capture of recombinant proteins from plant cell extract. On-line monitoring of recombinant protein …
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A cytochemical and biochemical study of acid hydrolases in developing plant cells, with particular reference to non-specific esterases
… and functions of acid hydrolases during plant cell division, differentiation and senescence using histochemical and biochemical procedures. Statistical analyses of histochemical data have shown that particles staining for naphthol AS BI phosphatase in dividing unfixed root cells of Vicia …
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Water permeability of plant cells measured by pressure probes: effects of light and turgor, and the role of unstirred layers
… (hydraulic conductivity) at the level of single cells, during measurements with the cell pressure probe (CPP) and (ii) on the use of the latter technique to investigate changes in water permeability of leaf cells in response to light. Internodes of the giant green alga Chara corallina and …
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From the Endoplasmic Reticulum to the Golgi Apparatus : In Vitro and In Vivo Approaches to Understanding COPII Vesicle Function in Plant Cells
… principles of ER-to-GA transport organization in plant cells are supposed to be similar to those in yeast and mammalian systems, evidence in support of such an assertion is largely circumstantial. Moreover, there is a substantial body of evidence that emphasizes the differences, such as the …
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Modification of a plant-based expression system to produce Lysosomal Acid Lipase (LAL) in plant cells using genetic modification of endogenous plant N-glycosylation
As plant cells are capable of complex N-glycosylation they are a valid expression system to produce safe and correctly glycosylated human proteins. To modify plant glycosylation patterns towards fully human patterns, the plant glycosylation machinery requires substitution of a series of plant …
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Optoperforation of Intact Plant Cells, Spectral Characterization of Alloy Disorder in InAsP Alloy Disorder in InAsP Alloys, and Bimetallic Concentric Surfaces for Metal-Enhanced Fluorescence in Upconverting Nanocrystals
… Despite being ostensibly restricted to mammalian cells, optoperforation was demonstrated in intact plant cells by means of successful femtosecond-laser-mediated infiltration of a membrane impermeable dextran-conjugated dye into cells of vital Arabidopsis seedling stems. By monitoring the rate of …
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Eine Untersuchung zur Endozytose in Pflanzenzellen
… of all eukaryotic life. In contrast to animal cells the endosomal system of plants is largely unknown. Recent research indicates a central role of endocytosis as a regulatory mechanism in plant morphogenesis and growth (Baluska et al., 2003; Geldner et al., 2003). Motivated by the growing …
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Identification and functional characterization of RXLR effector proteins that are conserved between downy mildew pathogens and Phytophthora species
Diverse pathogens secrete effector proteins into plant cells to manipulate host cellular processes. The genome of Hyaloperonospora arabidopsidis (Hpa), the causative agent of downy mildew of Arabidopsis, contains at least 134 candidate RXLR effector genes. These genes contain an RXLR motif required …
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