Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"pilus assembly"”.
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Molecular Analysis of Type IV Pilus Assembly in Clostridium perfringens
… insights into possible mechanisms of type IV pilus assembly. Toward that end, a pilin was purified from the Gram-negative pathogen Pseudomonas aeruginosa and incorporated into an artificial membrane. The pilin was probed by a solid state nuclear magnetic resonance (ssNMR) technique that can …
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Computationally and Experimentally Exploring the Type IV Pilus Assembly ATPase for Antivirulence Drug Discovery
… disease development. The bacterial type IV pilus (T4P) is a prevalent virulence factor in many ABR pathogens, contributing to bacterial pathogenesis by facilitating cell motility, surface adhesion, and biofilm formation. Critically, the T4P facilitates early stages of disease, providing a …
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The Type IV Pilus Assembly ATPase PilB as a Regulator of Biofilm Formation and an Antivirulence Target
… focus on understanding the regulation of the T4P assembly ATPase PilB. We examined its interactions with the secondary messenger cyclic-di-GMP (cdG). Specifically we investigated how cdG binding regulates PilB functions not only as the assembly ATPase, but also as an EPS signaling molecule in …
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Insights Into The Reactivation, Regulation and Essentiality of Oxidative Protein Folding Pathways In Actinobacteria
… oxidoreductase MdbA. MdbA is required for assembly of adhesive pilus, which mediate receptor-dependent bacterial interactions, or coaggregation, in <em>A. oris. </em>In the first part of this dissertation, I identify components of the electron transport chain (ETC) required for pilus …
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<em>Agrobacterium</em> Virb10 Contributions to Type Iv Substrate Secretion, T-Pilus Biogenesis, and Outer Membrane Pore Formation
… substrates to infected plant cells through assembly of a translocation channel and a surface structure termed a T-pilus. This thesis is focused on identifying contributions of VirB10 to substrate transfer and T-pilus formation through a mutational analysis. VirB10 is a bitopic protein with …
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Pilus biogenesis in uropathogenic Escherichia coli : An electrophysiology study on the PapC usher
… usher protein that functions as the site of pilus assembly and simultaneous translocation. The usher exists as a dimer, where each monomer consists of a gated β-barrel pore with several domains. To shed insight into the mechanistic details behind pilus biogenesis we instituted the first …
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Oxidative Protein Folding Pathways In Gram-Positive Actinobacteria
… secreted proteins in the exoplasm. Using the assembly of adhesive pili as a marker for disulfide bond formation in <em>A. oris</em> and <em>C. diphtheriae</em>, we found that protein oxidation is catalyzed by the membrane-bound MdbA. In <em>A. oris</em>, MdbA activity is maintained by VKOR, …
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Using prophylactics to improve resistance against Enterobacteriaceae in chickens
… aligned with plasmidic genes associated with pilus assembly and plasmid transfer, suggesting that certain smRNAs may drive plasmid transfer. These data identify the combination of probiotics and live Salmonella vaccine as a unique in vivo strategy to mitigate intestinal IncF plasmid transfer …
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Assembly and Display of Surface Proteins In Actinomyces Oris
… this thesis, I reveal mechanisms involved in the assembly and display of surface proteins that are central to these processes in <em>A. oris</em>.</p> <p>Cell wall-anchored proteins contain a signal peptide to direct their secretion to the exoplasmic side of the membrane, where they are liberated …