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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 48 for “"pilus"”.
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Understanding PilB, The Type IV Pilus (T4P) Assembly ATPase
The type IV pilus (T4P) is a dynamic long thin fiber found on the surface of many bacterial groups. T4P is a versatile nanomachine; it plays many important roles such as for surface attachment, virulence factor, and surface motility apparatus. This research focuses on understanding the kinetics of …
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Recruitment and Mechanism of the Bacterial Type IV Pilus Motors
The type IVa pilus system can be used for adhesion, natural competence, phage adsorption, folded-protein secretion, surface sensing, and surface associated motility. Likewise, it is a major virulence factor in many bacterial pathogens. This system extends and retracts a thin and long protein fibre …
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Molecular Analysis of Type IV Pilus Assembly in Clostridium perfringens
… insights into possible mechanisms of type IV pilus assembly. Toward that end, a pilin was purified from the Gram-negative pathogen Pseudomonas aeruginosa and incorporated into an artificial membrane. The pilin was probed by a solid state nuclear magnetic resonance (ssNMR) technique that can …
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Pilus biogenesis in uropathogenic Escherichia coli : An electrophysiology study on the PapC usher
… usher protein that functions as the site of pilus assembly and simultaneous translocation. The usher exists as a dimer, where each monomer consists of a gated β-barrel pore with several domains. To shed insight into the mechanistic details behind pilus biogenesis we instituted the first …
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Characterization of Type IV Pilus System Genes and Their Regulation in Clostridium perfringens
… pathogen that was found to contain Type IV pilus (T4P) system genes within the genomes of all its sequenced strains. T4P are widely used in Gram-negative organisms for aggregation, biofilm formation, adherence, and DNA uptake. Because few examples of T4P-utilizing Gram-positive bacteria are …
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Shear force impacts type IV pilus and flagellar dynamics during Pseudomonas aeruginosa surface adhesion
… demonstrate that cell tilting, driven by type IV pilus retraction, predicts surface departure at low shear forces. Conversely, higher host-relevant shear forces counteract cell tilting and enhance adhesion. Thus, P. aeruginosa couples type IV pilus dynamics and cell geometry to tune adhesion to …
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Computationally and Experimentally Exploring the Type IV Pilus Assembly ATPase for Antivirulence Drug Discovery
… disease development. The bacterial type IV pilus (T4P) is a prevalent virulence factor in many ABR pathogens, contributing to bacterial pathogenesis by facilitating cell motility, surface adhesion, and biofilm formation. Critically, the T4P facilitates early stages of disease, providing a …
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Pseudomonas aeruginosa Type IV pilus and resistance to the antimicrobial properties of surfactant protein-A
… In this study, we demonstrated that type IV pilus (Tfp) is important in the resistance of lung clearance both in the presence and absence of SP-A. The Tfp-deficient mutant, ΔpilA, is severely attenuated in an acute pneumonia model of infection in the lungs of wild-type mice that it allows …
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The Type IV Pilus Assembly ATPase PilB as a Regulator of Biofilm Formation and an Antivirulence Target
Bacterial type IV pili (T4P) are filamentous surface appendages with a variety of functions including motility, surface attachment, and biofilm formation. In many species of bacteria a clear understanding of how the functions of T4P in lifestyle switching are regulated remains to be elucidated. …
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<em>Agrobacterium</em> Virb10 Contributions to Type Iv Substrate Secretion, T-Pilus Biogenesis, and Outer Membrane Pore Formation
… channel and a surface structure termed a T-pilus. This thesis is focused on identifying contributions of VirB10 to substrate transfer and T-pilus formation through a mutational analysis. VirB10 is a bitopic protein with several domains, including a: (i) cytoplasmic N-terminus, (ii) single …
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Regulation of pilus production by the PilR/PilS two-component and Agr-like quorum sensing systems in Clostridium perfringens
… cause necrotic enteritis (NE) in poultry. Their pilus plays a role in adhesion and is important in pathogenesis. The NE pilus in these isolates consists of three pilins (CnaA, FimA, FimB) encoded by three genes that belong to the VR-10B allele of the VR-10 locus. There are two genes downstream of …
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Analyse von Protein-Protein Interaktionen im Typ IV Sekretionssystem von Agrobacterium tumefaciens
… Membran durchspannenden Proteinkomplex und einem Pilus (T-Pilus) assemblieren. Im Rahmen dieser Arbeit wurden Protein-Protein-Interaktionen im VirB/D4-Sekretionssystem von A. tumefaciens C58 analysiert, mit dem Ziel einer Aufklärung der Assemblierung von Transporterstruktur und T-Pilus. …
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Characterization of The Agrobacterium Tumefaciens Virb2 Pilin of The Virb/D4 Type Iv Secretion System
… channel spanning the cell envelope and a T-pilus extending from the cell surface. In A. tumefaciens, the VirB2 pilin subunit is required for assembly of the secretion channel and is the main subunit of the T-pilus. The focus of this thesis is to define key reactions associated with the T4SS …
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Molecular Basis of Corynebacterium Diphtheriae Virulence and Infection In The Caenorhabditis Elegans Model Host
… of C. diphtheriae possesses three distinct pilus structures, namely the SpaA, SpaD, and SpaH-type pili, which are encoded by three distinct pilus gene clusters. The pilus is assembled onto the bacterial peptidoglycan by a specific transpeptidase enzyme called sortase. Although the SpaA pili …
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Characterization of The Role of The Pkm101-Encoded T4Ss Outer Membrane Core Complex In Substrate Transfer, Pilus Biogenesis, and Recipient Cell Contact
… to recipient plant or bacterial cells, (ii) pilus biogenesis and donor-specific bacteriophage sensitivity, and (iii) recipient cell contact. My results demonstrate that the C-terminal tails of VirB10 and TraF<sub>pKM101</sub> are essential for DNA transfer and pilus biogenesis, whereas the AP …
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Von der Faltung zur Funktion: Steuerung des Infektionsmechanismus filamentöser Phagen durch Faltungsprozesse und Beschleunigung oxidativer Faltung durch Thioloxidasen
… bindet die N2 Domäne von G3P an den bakteriellen Pilus, beim Phagen IF1 an den I Pilus, beim Phagen fd an den F Pilus. Durch die spezifische Bindung an einen Pilus bestimmt die N2 Domäne von G3P die Wirtsspezifität des Phagen. Im zweiten Schritt der Infektion interagiert in beiden Fällen die N1 …
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Déterminants moléculaires de la spécificité du transfert conjugatif du plasmide TP114 dans le microbiote
… conditions expérimentales a révélé qu’un pilus accessoire était responsable de la stabilisation de la paire de conjugaison. Ce type de pilus s’avère crucial pour le transfert efficace dans des environnements instables, comme en bouillon/in vivo, où la mobilité bactérienne, les forces des …
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Phenotypic Suppression of "Escherichia coli" K88ac antigen by selective media and termperature variations
The K88ac antigen, thought to he a pilus on "Escherichia coli", has been determined to be one of the virulence factors in neonatal porcine colibacillosis. Using five different "E. coli" strains, the suppression of this antigen was studied on Tergitol-7(T7) and Nutrient Agar (Nut) at 18°C and 35°C. …
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Structure-Activity Relationship Studies of Mitochondrial Uncouplers and PilB Inhibitors
… enable them to cause disease such as the type IV pilus construction enzyme PilB, is a strategy of treatment. PilB is a highly conserved hexameric ATPase responsible for pilus generation and is thus obligatorily involved in bacterial type IV pilus use. Previously, the type IV pilus has been shown …
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